ID   NKD_ANOGA               Reviewed;         886 AA.
AC   Q08AA9; A7UV48;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2006, sequence version 1.
DT   03-AUG-2022, entry version 78.
DE   RecName: Full=Protein naked cuticle homolog;
GN   Name=nkd; ORFNames=AGAP009925;
OS   Anopheles gambiae (African malaria mosquito).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Nematocera; Culicoidea; Culicidae;
OC   Anophelinae; Anopheles.
OX   NCBI_TaxID=7165;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PEST;
RX   PubMed=12364791; DOI=10.1126/science.1076181;
RA   Holt R.A., Subramanian G.M., Halpern A., Sutton G.G., Charlab R.,
RA   Nusskern D.R., Wincker P., Clark A.G., Ribeiro J.M.C., Wides R.,
RA   Salzberg S.L., Loftus B.J., Yandell M.D., Majoros W.H., Rusch D.B., Lai Z.,
RA   Kraft C.L., Abril J.F., Anthouard V., Arensburger P., Atkinson P.W.,
RA   Baden H., de Berardinis V., Baldwin D., Benes V., Biedler J., Blass C.,
RA   Bolanos R., Boscus D., Barnstead M., Cai S., Center A., Chaturverdi K.,
RA   Christophides G.K., Chrystal M.A.M., Clamp M., Cravchik A., Curwen V.,
RA   Dana A., Delcher A., Dew I., Evans C.A., Flanigan M.,
RA   Grundschober-Freimoser A., Friedli L., Gu Z., Guan P., Guigo R.,
RA   Hillenmeyer M.E., Hladun S.L., Hogan J.R., Hong Y.S., Hoover J.,
RA   Jaillon O., Ke Z., Kodira C.D., Kokoza E., Koutsos A., Letunic I.,
RA   Levitsky A.A., Liang Y., Lin J.-J., Lobo N.F., Lopez J.R., Malek J.A.,
RA   McIntosh T.C., Meister S., Miller J.R., Mobarry C., Mongin E., Murphy S.D.,
RA   O'Brochta D.A., Pfannkoch C., Qi R., Regier M.A., Remington K., Shao H.,
RA   Sharakhova M.V., Sitter C.D., Shetty J., Smith T.J., Strong R., Sun J.,
RA   Thomasova D., Ton L.Q., Topalis P., Tu Z.J., Unger M.F., Walenz B.,
RA   Wang A.H., Wang J., Wang M., Wang X., Woodford K.J., Wortman J.R., Wu M.,
RA   Yao A., Zdobnov E.M., Zhang H., Zhao Q., Zhao S., Zhu S.C., Zhimulev I.,
RA   Coluzzi M., della Torre A., Roth C.W., Louis C., Kalush F., Mural R.J.,
RA   Myers E.W., Adams M.D., Smith H.O., Broder S., Gardner M.J., Fraser C.M.,
RA   Birney E., Bork P., Brey P.T., Venter J.C., Weissenbach J., Kafatos F.C.,
RA   Collins F.H., Hoffman S.L.;
RT   "The genome sequence of the malaria mosquito Anopheles gambiae.";
RL   Science 298:129-149(2002).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=16849595; DOI=10.1534/genetics.106.061853;
RA   Waldrop S., Chan C.-C., Cagatay T., Zhang S., Rousset R., Mack J.A.,
RA   Zeng W., Fish M.P., Zhang M., Amanai M., Wharton K.A. Jr.;
RT   "An unconventional nuclear localization motif is crucial for function of
RT   the Drosophila Wnt/wingless antagonist Naked cuticle.";
RL   Genetics 174:331-348(2006).
CC   -!- FUNCTION: Cell autonomous antagonist of the canonical Wnt signaling
CC       pathway. May activate a second Wnt signaling pathway that controls
CC       planar cell polarity. Required for neuroblast specification (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q9VVV9}.
CC       Cytoplasm {ECO:0000250|UniProtKB:Q9VVV9}. Nucleus
CC       {ECO:0000250|UniProtKB:Q9VVV9}.
CC   -!- SIMILARITY: Belongs to the NKD family. {ECO:0000305}.
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DR   EMBL; AAAB01008980; EDO63442.1; -; Genomic_DNA.
DR   EMBL; BK005845; DAA05784.1; -; mRNA.
DR   RefSeq; XP_001689169.1; XM_001689117.1.
DR   AlphaFoldDB; Q08AA9; -.
DR   STRING; 7165.AGAP009925-PA; -.
DR   PaxDb; Q08AA9; -.
DR   GeneID; 5668136; -.
DR   KEGG; aga:AgaP_AGAP009925; -.
DR   CTD; 5668136; -.
DR   VEuPathDB; VectorBase:AGAP009925; -.
DR   eggNOG; ENOG502QT1X; Eukaryota.
DR   HOGENOM; CLU_325475_0_0_1; -.
DR   InParanoid; Q08AA9; -.
DR   OMA; GNENCYE; -.
DR   OrthoDB; 426309at2759; -.
DR   PhylomeDB; Q08AA9; -.
DR   Proteomes; UP000007062; Chromosome 3R.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR   GO; GO:0090090; P:negative regulation of canonical Wnt signaling pathway; IEA:UniProt.
DR   GO; GO:0030178; P:negative regulation of Wnt signaling pathway; IBA:GO_Central.
DR   GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR   InterPro; IPR011992; EF-hand-dom_pair.
DR   InterPro; IPR002048; EF_hand_dom.
DR   InterPro; IPR040140; Nkd-like.
DR   PANTHER; PTHR22611; PTHR22611; 1.
DR   SUPFAM; SSF47473; SSF47473; 1.
DR   PROSITE; PS50222; EF_HAND_2; 1.
PE   2: Evidence at transcript level;
KW   Cell membrane; Cytoplasm; Developmental protein; Membrane; Metal-binding;
KW   Nucleus; Reference proteome; Wnt signaling pathway.
FT   CHAIN           1..886
FT                   /note="Protein naked cuticle homolog"
FT                   /id="PRO_0000301997"
FT   DOMAIN          181..216
FT                   /note="EF-hand"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT   REGION          62..149
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          267..286
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          307..338
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          554..583
FT                   /note="Required for nuclear localization and inhibition of
FT                   Wnt signaling"
FT                   /evidence="ECO:0000250"
FT   REGION          789..861
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        62..77
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        78..94
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        95..110
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   886 AA;  96858 MW;  31A1FD76BDCD068B CRC64;
     MAGNIVKWWK HKILGGYKQF TVLQECATDS EELIYPGRAP SACSAPPDLL LTSDREQMLK
     VKSSASTGSG TGQTKTSFQH SHHHHHHGHH HQSPSSNGHN HSGTSHQQQQ QQQHHHHHKS
     QKDAFRKCTN GKAGGGNATA GGASGAGATN TINTTAATIR SDPDRVRLEE FTCDVSLEDG
     KKPQPLQFSF TLYDLDGHGK ITKDDIAGIV STIYESIGKS VVVPHYGSKT INVRLTVSPD
     GKTATTKPAS AAVKKAIITP RRRYRSRKLI SDDDGSDTSE NCPRLLRNRT AATAVTNNGE
     TANNNQAALQ NQQQQQQQQH HHHHHHHSNG SSGKLKESNL SKATALAGDN QVNRSIGLSN
     SSEGSSAVGQ LLVEAYHKNN LSPGAANAGA GKGVLNNAPN ATKSKANENV YESINNLKCC
     NLQQQQQQHT ASNNTSLQTT ASSLPVTATG LNQSTTSTAA LICHDCVDGG APTTTLLPPL
     STLETVVIPA PSAGVTGRAK RKLVRKTRAS RKTAIAAKMM SEDFARRPRA RSLSVGNENC
     YENVIGRMAA AQEECWKSSL CRRELIEIIR ESMVKNSLCF QPNRRKEHHR LAAAVGATRS
     TQPTPVKLST ALLNQQYPNL SAEQKLTRSI NQVEQWLDHR SPKLVPKVKL ADDMQLQRTV
     VSRPLKRSKS KEELTPSNLL LLENLKISED IAEIAVVTPK KVYNKESLIA SATKKNIKTH
     HQHQHSPSRQ PTAVPTVAIG AEKPSAPLTV VNKQSIPVGD AQLVQLQYGS VPINADPSEC
     ENLIRMSDDG EDVEQHQHQQ HQQQQQTAPM AATKHSYRHH QPQSRSQPQS PQHHQHHHQQ
     EPRYCGSGRA HSVSSASAAS TTAVHRYVHE HIHHHYHHFE NDPDES