NKG2D_PONPY
ID NKG2D_PONPY Reviewed; 216 AA.
AC Q8MJH1; Q8MJH0;
DT 06-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=NKG2-D type II integral membrane protein;
DE AltName: Full=Killer cell lectin-like receptor subfamily K member 1;
DE AltName: Full=NK cell receptor D;
DE AltName: Full=NKG2-D-activating NK receptor;
DE AltName: CD_antigen=CD314;
GN Name=KLRK1; Synonyms=NKG2D;
OS Pongo pygmaeus (Bornean orangutan).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Pongo.
OX NCBI_TaxID=9600;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ILE-61.
RX PubMed=12077248; DOI=10.4049/jimmunol.169.1.220;
RA Guethlein L.A., Flodin L.R., Adams E.J., Parham P.;
RT "NK cell receptors of the orangutan (Pongo pygmaeus): a pivotal species for
RT tracking the coevolution of killer cell Ig-like receptors with MHC-C.";
RL J. Immunol. 169:220-229(2002).
CC -!- FUNCTION: Functions as an activating and costimulatory receptor
CC involved in immunosurveillance upon binding to various cellular stress-
CC inducible ligands displayed at the surface of autologous tumor cells
CC and virus-infected cells. Provides both stimulatory and costimulatory
CC innate immune responses on activated killer (NK) cells, leading to
CC cytotoxic activity. Acts as a costimulatory receptor for T-cell
CC receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by
CC amplifying T-cell activation. Stimulates perforin-mediated elimination
CC of ligand-expressing tumor cells. Signaling involves calcium influx,
CC culminating in the expression of TNF-alpha. Participates in NK cell-
CC mediated bone marrow graft rejection. May play a regulatory role in
CC differentiation and survival of NK cells. Binds to ligands belonging to
CC various subfamilies of MHC class I-related glycoproteins (By
CC similarity). {ECO:0000250}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Heterohexamer composed of two
CC subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via
CC transmembrane domain) with HCST/DAP10 (via transmembrane domain); the
CC interaction is required for KLRK1 NK cell surface and induces NK cell-
CC mediated cytotoxicity. Can form disulfide-bonded heterodimer with CD94
CC (By similarity). Interacts with CEACAM1; recruits PTPN6 that
CC dephosphorylates VAV1 (By similarity). {ECO:0000250,
CC ECO:0000250|UniProtKB:P26718}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Single-pass type II
CC membrane protein {ECO:0000250}. Note=Colocalized with HCST on the cell
CC surface. {ECO:0000250}.
CC -!- MISCELLANEOUS: Is not capable of signal transduction by itself, but
CC operates through the adapter protein HCST. {ECO:0000250}.
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DR EMBL; AF470403; AAM78503.1; -; mRNA.
DR EMBL; AF470404; AAM78504.1; -; mRNA.
DR AlphaFoldDB; Q8MJH1; -.
DR SMR; Q8MJH1; -.
DR GO; GO:0009986; C:cell surface; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0030246; F:carbohydrate binding; IEA:UniProtKB-KW.
DR GO; GO:0002250; P:adaptive immune response; IEA:UniProtKB-KW.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0045954; P:positive regulation of natural killer cell mediated cytotoxicity; ISS:UniProtKB.
DR CDD; cd03593; CLECT_NK_receptors_like; 1.
DR Gene3D; 3.10.100.10; -; 1.
DR InterPro; IPR001304; C-type_lectin-like.
DR InterPro; IPR016186; C-type_lectin-like/link_sf.
DR InterPro; IPR016187; CTDL_fold.
DR InterPro; IPR042169; NKG2D.
DR InterPro; IPR033992; NKR-like_CTLD.
DR PANTHER; PTHR47494; PTHR47494; 1.
DR Pfam; PF00059; Lectin_C; 1.
DR SMART; SM00034; CLECT; 1.
DR SUPFAM; SSF56436; SSF56436; 1.
DR PROSITE; PS50041; C_TYPE_LECTIN_2; 1.
PE 2: Evidence at transcript level;
KW Adaptive immunity; Cell membrane; Differentiation; Disulfide bond;
KW Glycoprotein; Immunity; Innate immunity; Lectin; Membrane; Receptor;
KW Signal-anchor; Transmembrane; Transmembrane helix.
FT CHAIN 1..216
FT /note="NKG2-D type II integral membrane protein"
FT /id="PRO_0000274560"
FT TOPO_DOM 1..51
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 52..72
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..216
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 98..213
FT /note="C-type lectin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 131
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 163
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 202
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 96..105
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 99..110
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 127..211
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT DISULFID 189..203
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00040"
FT VARIANT 61
FT /note="V -> I (in allele NKG2-D*02)"
FT /evidence="ECO:0000269|PubMed:12077248"
FT /id="VAR_030319"
SQ SEQUENCE 216 AA; 25211 MW; 92F4EF44924903FF CRC64;
MGWIRGRRSR HSWEMSEFHN YNLDLAKNDF STRWQKQRCP VIKSKCRENT SPLFFCCFIA
VAMGIRFIVM VTIWSAVFLN SLFNQEVQIP LTGSYCGPCP KNWICYKNNC YQFFNESKNW
YESQASCMSQ NASLLKVYSK EDQDLLKLVK SYHWMGLIHI PTNGSWQWED GSILSPNLLT
IIEMQKGDCA LYASSFKGYI ENCSTPNTYI CMQRTV
