NKG7_HUMAN
ID NKG7_HUMAN Reviewed; 165 AA.
AC Q16617;
DT 15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Protein NKG7;
DE AltName: Full=G-CSF-induced gene 1 protein;
DE Short=GIG-1 protein;
DE AltName: Full=Granule membrane protein of 17 kDa;
DE Short=GMP-17;
DE AltName: Full=Natural killer cell protein 7;
DE AltName: Full=p15-TIA-1;
GN Name=NKG7; Synonyms=GIG1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=8458737; DOI=10.1016/0198-8859(93)90006-m;
RA Turman M.A., Yabe T., McSherry C., Bach F.H., Houchins J.P.;
RT "Characterization of a novel gene (NKG7) on human chromosome 19 that is
RT expressed in natural killer cells and T cells.";
RL Hum. Immunol. 36:34-40(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
RX PubMed=7510105; DOI=10.1006/bbrc.1994.1187;
RA Shimane M., Tani K., Maruyama K., Takahashi S., Ozawa K., Asano S.;
RT "Molecular cloning and characterization of G-CSF induced gene cDNA.";
RL Biochem. Biophys. Res. Commun. 199:26-32(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Church R.L., Li X.L., Wang J.H.;
RT "Human chromosome 19q13.4 DNA sequence, including complete sequence for
RT LIM2 and NKG7.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Blood;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PROTEIN SEQUENCE OF 1-25, AND SUBCELLULAR LOCATION.
RX PubMed=8570616; DOI=10.1073/pnas.93.2.685;
RA Medley Q.G., Kedersha N., O'Brien S., Tian Q., Schlossman S.F., Streuli M.,
RA Anderson P.;
RT "Characterization of GMP-17, a granule membrane protein that moves to the
RT plasma membrane of natural killer cells following target cell
RT recognition.";
RL Proc. Natl. Acad. Sci. U.S.A. 93:685-689(1996).
RN [6]
RP INDUCTION.
RX PubMed=32839608; DOI=10.1038/s41590-020-0758-6;
RA Ng S.S., De Labastida Rivera F., Yan J., Corvino D., Das I., Zhang P.,
RA Kuns R., Chauhan S.B., Hou J., Li X.Y., Frame T.C.M., McEnroe B.A.,
RA Moore E., Na J., Engel J.A., Soon M.S.F., Singh B., Kueh A.J., Herold M.J.,
RA Montes de Oca M., Singh S.S., Bunn P.T., Aguilera A.R., Casey M., Braun M.,
RA Ghazanfari N., Wani S., Wang Y., Amante F.H., Edwards C.L., Haque A.,
RA Dougall W.C., Singh O.P., Baxter A.G., Teng M.W.L., Loukas A., Daly N.L.,
RA Cloonan N., Degli-Esposti M.A., Uzonna J., Heath W.R., Bald T., Tey S.K.,
RA Nakamura K., Hill G.R., Kumar R., Sundar S., Smyth M.J., Engwerda C.R.;
RT "The NK cell granule protein NKG7 regulates cytotoxic granule exocytosis
RT and inflammation.";
RL Nat. Immunol. 21:1205-1218(2020).
CC -!- FUNCTION: Regulates cytotoxic granule exocytosis in effector
CC lymphocytes, thus acting as a critical mediator of inflammation in a
CC broad range of infectious and non-infectious diseases (By similarity).
CC Essential for cytotoxic degranulation of natural killer (NK) cells and
CC CD8(+) T-cells and for the activation of CD4(+) T-cells following
CC infection (By similarity). Plays a critical role in CD8(+) T-cell and
CC NK cell-mediated cytolysis of target cells and contributes to the
CC cytolytic activity via the perforin/granzyme pathway by enhancing
CC exocytosis of LAMP1-carrying lytic granules (By similarity).
CC Contributes to NK cell-mediated control of cancer metastasis (By
CC similarity). {ECO:0000250|UniProtKB:Q99PA5}.
CC -!- INTERACTION:
CC Q16617; Q6RW13: AGTRAP; NbExp=3; IntAct=EBI-3919611, EBI-741181;
CC Q16617; Q6RW13-2: AGTRAP; NbExp=3; IntAct=EBI-3919611, EBI-11522760;
CC Q16617; Q86WK6: AMIGO1; NbExp=3; IntAct=EBI-3919611, EBI-19125216;
CC Q16617; Q13520: AQP6; NbExp=3; IntAct=EBI-3919611, EBI-13059134;
CC Q16617; Q3SXY8: ARL13B; NbExp=3; IntAct=EBI-3919611, EBI-11343438;
CC Q16617; O75787: ATP6AP2; NbExp=3; IntAct=EBI-3919611, EBI-2512037;
CC Q16617; Q13145: BAMBI; NbExp=3; IntAct=EBI-3919611, EBI-742991;
CC Q16617; P19397: CD53; NbExp=3; IntAct=EBI-3919611, EBI-6657396;
CC Q16617; P11912: CD79A; NbExp=3; IntAct=EBI-3919611, EBI-7797864;
CC Q16617; O95471: CLDN7; NbExp=3; IntAct=EBI-3919611, EBI-740744;
CC Q16617; Q9UHP7-3: CLEC2D; NbExp=3; IntAct=EBI-3919611, EBI-11749983;
CC Q16617; Q9ULY5: CLEC4E; NbExp=4; IntAct=EBI-3919611, EBI-12807010;
CC Q16617; Q9BQT9: CLSTN3; NbExp=3; IntAct=EBI-3919611, EBI-11291074;
CC Q16617; Q15125: EBP; NbExp=3; IntAct=EBI-3919611, EBI-3915253;
CC Q16617; Q9HAV5: EDA2R; NbExp=3; IntAct=EBI-3919611, EBI-526033;
CC Q16617; Q9Y282: ERGIC3; NbExp=3; IntAct=EBI-3919611, EBI-781551;
CC Q16617; Q96KR6: FAM210B; NbExp=3; IntAct=EBI-3919611, EBI-18938272;
CC Q16617; O15552: FFAR2; NbExp=3; IntAct=EBI-3919611, EBI-2833872;
CC Q16617; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-3919611, EBI-12142257;
CC Q16617; P36382: GJA5; NbExp=3; IntAct=EBI-3919611, EBI-750433;
CC Q16617; Q8TED1: GPX8; NbExp=3; IntAct=EBI-3919611, EBI-11721746;
CC Q16617; Q6UXK2: ISLR2; NbExp=3; IntAct=EBI-3919611, EBI-1266923;
CC Q16617; P26715: KLRC1; NbExp=3; IntAct=EBI-3919611, EBI-9018187;
CC Q16617; Q8TAF8: LHFPL5; NbExp=3; IntAct=EBI-3919611, EBI-2820517;
CC Q16617; Q6ZSS7: MFSD6; NbExp=3; IntAct=EBI-3919611, EBI-2858252;
CC Q16617; O14880: MGST3; NbExp=3; IntAct=EBI-3919611, EBI-724754;
CC Q16617; Q96HJ5: MS4A3; NbExp=3; IntAct=EBI-3919611, EBI-12806656;
CC Q16617; P21757: MSR1; NbExp=7; IntAct=EBI-3919611, EBI-1776976;
CC Q16617; P15941-11: MUC1; NbExp=3; IntAct=EBI-3919611, EBI-17263240;
CC Q16617; P41145: OPRK1; NbExp=3; IntAct=EBI-3919611, EBI-925028;
CC Q16617; O00623: PEX12; NbExp=3; IntAct=EBI-3919611, EBI-594836;
CC Q16617; O60894: RAMP1; NbExp=3; IntAct=EBI-3919611, EBI-962893;
CC Q16617; Q9Y225-2: RNF24; NbExp=3; IntAct=EBI-3919611, EBI-13044680;
CC Q16617; Q05940: SLC18A2; NbExp=3; IntAct=EBI-3919611, EBI-18036244;
CC Q16617; Q8IWU4: SLC30A8; NbExp=3; IntAct=EBI-3919611, EBI-10262251;
CC Q16617; P27105: STOM; NbExp=5; IntAct=EBI-3919611, EBI-1211440;
CC Q16617; Q16623: STX1A; NbExp=4; IntAct=EBI-3919611, EBI-712466;
CC Q16617; Q96MV1: TLCD4; NbExp=3; IntAct=EBI-3919611, EBI-12947623;
CC Q16617; Q9BVX2: TMEM106C; NbExp=3; IntAct=EBI-3919611, EBI-2821497;
CC Q16617; Q8N3G9: TMEM130; NbExp=3; IntAct=EBI-3919611, EBI-19763514;
CC Q16617; Q8IV31: TMEM139; NbExp=3; IntAct=EBI-3919611, EBI-7238458;
CC Q16617; Q96HE8: TMEM80; NbExp=3; IntAct=EBI-3919611, EBI-11742770;
CC Q16617; Q6PEY1: TMEM88; NbExp=3; IntAct=EBI-3919611, EBI-17198826;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:8570616};
CC Multi-pass membrane protein {ECO:0000269|PubMed:8570616}. Cytolytic
CC granule membrane {ECO:0000269|PubMed:8570616}; Multi-pass membrane
CC protein {ECO:0000269|PubMed:8570616}.
CC -!- TISSUE SPECIFICITY: Expressed in activated T-cells, in kidney, liver,
CC lung and pancreas. Not expressed in brain, heart, or skeletal muscle.
CC Expressed at high levels in TCR gamma delta-expressing CTL clones, and
CC in some TCR alpha beta-expressing CTL clones (both CD4+ and CD8+), but
CC is not expressed in other TCR alpha beta-expressing CTL clones and in
CC cell lines representing B-cells, monocytes, and myeloid cells.
CC -!- INDUCTION: By CSF3/G-CSF (PubMed:7510105). Up-regulated in CD4(+) T-
CC cells from peripheral blood mononuclear cells of patients with visceral
CC leishmaniasis (PubMed:32839608). {ECO:0000269|PubMed:32839608,
CC ECO:0000269|PubMed:7510105}.
CC -!- SIMILARITY: Belongs to the PMP-22/EMP/MP20 family. {ECO:0000305}.
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DR EMBL; U09608; AAA18209.1; -; mRNA.
DR EMBL; S69115; AAB30078.1; -; mRNA.
DR EMBL; AF305941; AAG32329.1; -; Genomic_DNA.
DR EMBL; BC015759; AAH15759.1; -; mRNA.
DR CCDS; CCDS12830.1; -.
DR PIR; JC2081; JC2081.
DR RefSeq; NP_005592.1; NM_005601.3.
DR AlphaFoldDB; Q16617; -.
DR SMR; Q16617; -.
DR BioGRID; 110883; 46.
DR IntAct; Q16617; 45.
DR STRING; 9606.ENSP00000221978; -.
DR PhosphoSitePlus; Q16617; -.
DR BioMuta; NKG7; -.
DR DMDM; 3914144; -.
DR MassIVE; Q16617; -.
DR PaxDb; Q16617; -.
DR PeptideAtlas; Q16617; -.
DR PRIDE; Q16617; -.
DR Antibodypedia; 76657; 35 antibodies from 11 providers.
DR DNASU; 4818; -.
DR Ensembl; ENST00000221978.10; ENSP00000221978.4; ENSG00000105374.10.
DR GeneID; 4818; -.
DR KEGG; hsa:4818; -.
DR MANE-Select; ENST00000221978.10; ENSP00000221978.4; NM_005601.4; NP_005592.1.
DR UCSC; uc002pwj.4; human.
DR CTD; 4818; -.
DR DisGeNET; 4818; -.
DR GeneCards; NKG7; -.
DR HGNC; HGNC:7830; NKG7.
DR HPA; ENSG00000105374; Group enriched (bone marrow, lymphoid tissue).
DR MIM; 606008; gene.
DR neXtProt; NX_Q16617; -.
DR OpenTargets; ENSG00000105374; -.
DR PharmGKB; PA31639; -.
DR VEuPathDB; HostDB:ENSG00000105374; -.
DR eggNOG; ENOG502SUH4; Eukaryota.
DR GeneTree; ENSGT01050000244814; -.
DR HOGENOM; CLU_133330_0_0_1; -.
DR InParanoid; Q16617; -.
DR OMA; YIHVTQT; -.
DR OrthoDB; 1488313at2759; -.
DR PhylomeDB; Q16617; -.
DR TreeFam; TF330587; -.
DR PathwayCommons; Q16617; -.
DR SignaLink; Q16617; -.
DR BioGRID-ORCS; 4818; 5 hits in 1067 CRISPR screens.
DR GenomeRNAi; 4818; -.
DR Pharos; Q16617; Tdark.
DR PRO; PR:Q16617; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q16617; protein.
DR Bgee; ENSG00000105374; Expressed in granulocyte and 103 other tissues.
DR ExpressionAtlas; Q16617; baseline and differential.
DR Genevisible; Q16617; HS.
DR GO; GO:0044194; C:cytolytic granule; ISS:UniProtKB.
DR GO; GO:0101004; C:cytolytic granule membrane; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; TAS:ProtInc.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0035710; P:CD4-positive, alpha-beta T cell activation; ISS:UniProtKB.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042832; P:defense response to protozoan; IDA:UniProtKB.
DR GO; GO:0140507; P:granzyme-mediated programmed cell death signaling pathway; ISS:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0043320; P:natural killer cell degranulation; ISS:UniProtKB.
DR GO; GO:0042267; P:natural killer cell mediated cytotoxicity; ISS:UniProtKB.
DR GO; GO:0002420; P:natural killer cell mediated cytotoxicity directed against tumor cell target; ISS:UniProtKB.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISS:UniProtKB.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR004032; PMP22_EMP_MP20.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PROSITE; PS01221; PMP22_1; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Cytolysis; Direct protein sequencing; Inflammatory response;
KW Lysosome; Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..165
FT /note="Protein NKG7"
FT /id="PRO_0000164667"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 165 AA; 17665 MW; CEE2901B6DC42A8C CRC64;
MELCRSLALL GGSLGLMFCL IALSTDFWFE AVGPTHSAHS GLWPTGHGDI ISGYIHVTQT
FSIMAVLWAL VSVSFLVLSC FPSLFPPGHG PLVSTTAAFA AAISMVVAMA VYTSERWDQP
PHPQIQTFFS WSFYLGWVSA ILLLCTGALS LGAHCGGPRP GYETL
