NKG7_MOUSE
ID NKG7_MOUSE Reviewed; 165 AA.
AC Q99PA5;
DT 10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 89.
DE RecName: Full=Protein NKG7;
DE AltName: Full=Natural killer cell protein 7;
GN Name=Nkg7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129/SvJ;
RX PubMed=11290961;
RA Zhou L., Li X.L., Church R.L.;
RT "The mouse lens fiber-cell intrinsic membrane protein MP19 gene (Lim2) and
RT granule membrane protein GMP-17 gene (Nkg7): isolation and sequence
RT analysis of two neighboring genes.";
RL Mol. Vis. 7:79-88(2001).
RN [2]
RP FUNCTION, DISRUPTION PHENOTYPE, INDUCTION, SUBCELLULAR LOCATION, AND TISSUE
RP SPECIFICITY.
RX PubMed=32839608; DOI=10.1038/s41590-020-0758-6;
RA Ng S.S., De Labastida Rivera F., Yan J., Corvino D., Das I., Zhang P.,
RA Kuns R., Chauhan S.B., Hou J., Li X.Y., Frame T.C.M., McEnroe B.A.,
RA Moore E., Na J., Engel J.A., Soon M.S.F., Singh B., Kueh A.J., Herold M.J.,
RA Montes de Oca M., Singh S.S., Bunn P.T., Aguilera A.R., Casey M., Braun M.,
RA Ghazanfari N., Wani S., Wang Y., Amante F.H., Edwards C.L., Haque A.,
RA Dougall W.C., Singh O.P., Baxter A.G., Teng M.W.L., Loukas A., Daly N.L.,
RA Cloonan N., Degli-Esposti M.A., Uzonna J., Heath W.R., Bald T., Tey S.K.,
RA Nakamura K., Hill G.R., Kumar R., Sundar S., Smyth M.J., Engwerda C.R.;
RT "The NK cell granule protein NKG7 regulates cytotoxic granule exocytosis
RT and inflammation.";
RL Nat. Immunol. 21:1205-1218(2020).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=33911019; DOI=10.4049/immunohorizons.2100029;
RA Morikawa Y., Murakami M., Kondo H., Nemoto N., Iwabuchi K., Eshima K.;
RT "Natural Killer Cell Group 7 Sequence in Cytotoxic Cells Optimizes
RT Exocytosis of Lytic Granules Essential for the Perforin-Dependent, but Not
RT Fas Ligand-Dependent, Cytolytic Pathway.";
RL Immunohorizons 5:234-245(2021).
CC -!- FUNCTION: Regulates cytotoxic granule exocytosis in effector
CC lymphocytes, thus acting as a critical mediator of inflammation in a
CC broad range of infectious and non-infectious diseases
CC (PubMed:32839608). Essential for cytotoxic degranulation of natural
CC killer (NK) cells and CD8(+) T-cells and for the activation of CD4(+)
CC T-cells following infection (PubMed:32839608). Plays a critical role in
CC CD8(+) T-cell and NK cell-mediated cytolysis of target cells and
CC contributes to the cytolytic activity via the perforin/granzyme pathway
CC by enhancing exocytosis of LAMP1-carrying lytic granules
CC (PubMed:32839608, PubMed:33911019). Contributes to NK cell-mediated
CC control of cancer metastasis (PubMed:32839608).
CC {ECO:0000269|PubMed:32839608, ECO:0000269|PubMed:33911019}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q16617};
CC Multi-pass membrane protein {ECO:0000255}. Cytolytic granule membrane
CC {ECO:0000305|PubMed:32839608}; Multi-pass membrane protein
CC {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Predominantly expressed by leukocytes with
CC cytotoxic activity such as CD8(+) T-cells and natural killer cells.
CC {ECO:0000269|PubMed:32839608, ECO:0000269|PubMed:33911019}.
CC -!- INDUCTION: Up-regulated in CD4(+)T-cells in the liver and spleen upon
CC infection with Leishmania donovani. {ECO:0000269|PubMed:32839608}.
CC -!- DISRUPTION PHENOTYPE: Cytotoxic activity of natural killer cells and
CC CD8(+) T-cells are defective in knockout mice (PubMed:32839608,
CC PubMed:33911019). Mice fail to resolve Leishmania donovani infection,
CC which is accompanied by a sustained reduction in the production of pro-
CC inflammatory cytokines and a defective CD4(+) T-cell response
CC (PubMed:32839608). Mice infected with Plasmodium berghei ANKA show a
CC significant reduction in the recruitment of parasite-specific cytotoxic
CC CD8(+) T cells to the brain, along with lower levels of inflammation
CC (PubMed:32839608). Mice injected intravenously with B16F10 and LWT1
CC melanoma cell lines show a significant increase in the number of lung
CC metastases compared with wild-type mice and an vitro impairment of
CC natural killer cells to lyse susceptible tumor cells (PubMed:32839608).
CC {ECO:0000269|PubMed:32839608, ECO:0000269|PubMed:33911019}.
CC -!- SIMILARITY: Belongs to the PMP-22/EMP/MP20 family. {ECO:0000305}.
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DR EMBL; AF320075; AAK08063.1; -; Genomic_DNA.
DR CCDS; CCDS21169.1; -.
DR AlphaFoldDB; Q99PA5; -.
DR SMR; Q99PA5; -.
DR STRING; 10090.ENSMUSP00000068946; -.
DR iPTMnet; Q99PA5; -.
DR PhosphoSitePlus; Q99PA5; -.
DR PaxDb; Q99PA5; -.
DR PRIDE; Q99PA5; -.
DR MGI; MGI:1931250; Nkg7.
DR eggNOG; ENOG502SUH4; Eukaryota.
DR InParanoid; Q99PA5; -.
DR PhylomeDB; Q99PA5; -.
DR PRO; PR:Q99PA5; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q99PA5; protein.
DR GO; GO:0044194; C:cytolytic granule; IDA:UniProtKB.
DR GO; GO:0101004; C:cytolytic granule membrane; ISS:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; ISS:UniProtKB.
DR GO; GO:0035710; P:CD4-positive, alpha-beta T cell activation; IMP:UniProtKB.
DR GO; GO:0019835; P:cytolysis; IEA:UniProtKB-KW.
DR GO; GO:0042832; P:defense response to protozoan; IDA:UniProtKB.
DR GO; GO:0140507; P:granzyme-mediated programmed cell death signaling pathway; IMP:UniProtKB.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0043320; P:natural killer cell degranulation; IMP:UniProtKB.
DR GO; GO:0042267; P:natural killer cell mediated cytotoxicity; IMP:UniProtKB.
DR GO; GO:0002420; P:natural killer cell mediated cytotoxicity directed against tumor cell target; IMP:UniProtKB.
DR GO; GO:0050729; P:positive regulation of inflammatory response; IMP:UniProtKB.
DR InterPro; IPR004031; PMP22/EMP/MP20/Claudin.
DR InterPro; IPR004032; PMP22_EMP_MP20.
DR Pfam; PF00822; PMP22_Claudin; 1.
DR PROSITE; PS01222; PMP22_2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytolysis; Inflammatory response; Lysosome; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..165
FT /note="Protein NKG7"
FT /id="PRO_0000164668"
FT TRANSMEM 9..29
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 165 AA; 17958 MW; FD04B090FCD8AA43 CRC64;
MEPCRSLALF AGSLGLTSSL IALTTDFWIV ATGPHFSAHS GLWPTSQETQ VAGYIHVTQS
FCILAVLWGL VSVSFLILSC IPALSAPGRG PLVSTVMAFS AALSILVAMA VYTSMRWSQT
PFSQVQTFFS WSFYLGWVSF ILFLFAGCLS LGAHCRTRRA EYETL
