ID   NKL_PIG                 Reviewed;         129 AA.
AC   Q29075;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=Antimicrobial peptide NK-lysin;
DE            Short=NKL;
DE   Flags: Precursor; Fragment;
GN   Name=NKL;
OS   Sus scrofa (Pig).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX   NCBI_TaxID=9823;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 47-124.
RC   TISSUE=Bone marrow, and Small intestine;
RX   PubMed=7737114; DOI=10.1002/j.1460-2075.1995.tb07150.x;
RA   Andersson M., Gunne H., Agerberth B., Boman A., Bergman T., Sillard R.,
RA   Joernvall H., Mutt V., Olsson B., Wigzell H., Dagerlind A., Boman H.G.,
RA   Gudmundsson G.H.;
RT   "NK-lysin, a novel effector peptide of cytotoxic T and NK cells. Structure
RT   and cDNA cloning of the porcine form, induction by interleukin 2,
RT   antibacterial and antitumour activity.";
RL   EMBO J. 14:1615-1625(1995).
RN   [2]
RP   STRUCTURE BY NMR OF 47-124.
RX   PubMed=9334742; DOI=10.1038/nsb1097-793;
RA   Liepinsh E., Andersson M., Ruysschaert J.-M., Otting G.;
RT   "Saposin fold revealed by the NMR structure of NK-lysin.";
RL   Nat. Struct. Biol. 4:793-795(1997).
CC   -!- FUNCTION: May be an effector molecule of cytotoxic activity. High
CC       activity against E.coli and B.megaterium, moderate against
CC       A.calcoaceticus and S.pyogenes. No activity against P.aeruginosa,
CC       S.aureus and Salmonella. Has some antifungal activity against
CC       C.albicans.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Cytotoxic T and NK cells.
CC   -!- INDUCTION: By interleukin-2.
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DR   EMBL; X85431; CAA59720.1; -; mRNA.
DR   PIR; S55044; S55044.
DR   PDB; 1NKL; NMR; -; A=47-124.
DR   PDBsum; 1NKL; -.
DR   AlphaFoldDB; Q29075; -.
DR   SMR; Q29075; -.
DR   STRING; 9823.ENSSSCP00000008784; -.
DR   TCDB; 1.C.35.3.1; the amoebapore (amoebapore) family.
DR   PaxDb; Q29075; -.
DR   PeptideAtlas; Q29075; -.
DR   eggNOG; ENOG502ST3Z; Eukaryota.
DR   InParanoid; Q29075; -.
DR   EvolutionaryTrace; Q29075; -.
DR   Proteomes; UP000008227; Unplaced.
DR   Proteomes; UP000314985; Unplaced.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; IBA:GO_Central.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IBA:GO_Central.
DR   GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR   InterPro; IPR038847; Granulysin-like.
DR   InterPro; IPR007856; SapB_1.
DR   InterPro; IPR008138; SapB_2.
DR   InterPro; IPR011001; Saposin-like.
DR   InterPro; IPR008139; SaposinB_dom.
DR   PANTHER; PTHR15541; PTHR15541; 1.
DR   Pfam; PF05184; SapB_1; 1.
DR   Pfam; PF03489; SapB_2; 1.
DR   SMART; SM00741; SapB; 1.
DR   SUPFAM; SSF47862; SSF47862; 1.
DR   PROSITE; PS50015; SAP_B; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Antibiotic; Antimicrobial; Direct protein sequencing;
KW   Disulfide bond; Fungicide; Reference proteome; Secreted; Signal.
FT   SIGNAL          <1..6
FT                   /evidence="ECO:0000255"
FT   PROPEP          7..46
FT                   /evidence="ECO:0000269|PubMed:7737114"
FT                   /id="PRO_0000031663"
FT   PEPTIDE         47..124
FT                   /note="Antimicrobial peptide NK-lysin"
FT                   /id="PRO_0000031664"
FT   PROPEP          125..129
FT                   /id="PRO_0000031665"
FT   DOMAIN          46..126
FT                   /note="Saposin B-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT   DISULFID        50..122
FT   DISULFID        53..116
FT   DISULFID        81..91
FT   VARIANT         48..49
FT                   /note="LI -> YF"
FT   VARIANT         79
FT                   /note="R -> Q"
FT   VARIANT         84
FT                   /note="M -> L"
FT   VARIANT         90
FT                   /note="V -> L"
FT   VARIANT         97
FT                   /note="T -> S"
FT   VARIANT         104
FT                   /note="K -> W"
FT   NON_TER         1
FT   HELIX           49..64
FT                   /evidence="ECO:0007829|PDB:1NKL"
FT   HELIX           70..83
FT                   /evidence="ECO:0007829|PDB:1NKL"
FT   HELIX           88..107
FT                   /evidence="ECO:0007829|PDB:1NKL"
FT   HELIX           112..118
FT                   /evidence="ECO:0007829|PDB:1NKL"
SQ   SEQUENCE   129 AA;  14317 MW;  4A87B206E20C53AD CRC64;
     PGLAFSGLTP EHSALARAHP CDGEQFCQNL APEDPQGDQL LQREELGLIC ESCRKIIQKL
     EDMVGPQPNE DTVTQAASRV CDKMKILRGV CKKIMRTFLR RISKDILTGK KPQAICVDIK
     ICKEKTGLI