ID   NKP2_SCHPO              Reviewed;         188 AA.
AC   O13952;
DT   14-OCT-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1998, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Inner kinetochore subunit cnl2;
DE   AltName: Full=Centromere-localized protein 2;
DE   AltName: Full=Constitutive centromere-associated network protein cnl2;
GN   Name=cnl2; ORFNames=SPAC23H4.11c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   SUBCELLULAR LOCATION, AND FUNCTION.
RX   PubMed=17035632; DOI=10.1091/mbc.e06-05-0388;
RA   Hayashi A., Asakawa H., Haraguchi T., Hiraoka Y.;
RT   "Reconstruction of the kinetochore during meiosis in fission yeast
RT   Schizosaccharomyces pombe.";
RL   Mol. Biol. Cell 17:5173-5184(2006).
RN   [3]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=16823372; DOI=10.1038/nbt1222;
RA   Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA   Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA   Yoshida M.;
RT   "ORFeome cloning and global analysis of protein localization in the fission
RT   yeast Schizosaccharomyces pombe.";
RL   Nat. Biotechnol. 24:841-847(2006).
CC   -!- FUNCTION: Component of the kinetochore, a multiprotein complex that
CC       assembles on centromeric DNA and attaches chromosomes to spindle
CC       microtubules, mediating chromosome segregation and sister chromatid
CC       segregation during meiosis and mitosis. Component of the inner
CC       kinetochore constitutive centromere-associated network (CCAN), which
CC       serves as a structural platform for outer kinetochore assembly.
CC       {ECO:0000269|PubMed:17035632}.
CC   -!- SUBUNIT: Component of the inner kinetochore constitutive centromere-
CC       associated network (CCAN) (also known as central kinetochore Sim4
CC       complex in fission yeast), which is composed of at least cnl2, cnp3,
CC       cnp20, fta1, fta2, fta3, fta4, fta6, fta7, mal2, mhf1, mhf2, mis6,
CC       mis15, mis17, sim4 and wip1. {ECO:0000250|UniProtKB:Q12493}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Chromosome, centromere,
CC       kinetochore. Note=Localized at the central region, but not at the outer
CC       repeats, of the centromere and remains at the centromere during mitosis
CC       and meiosis.
CC   -!- SIMILARITY: Belongs to the NKP2 family. {ECO:0000305}.
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DR   EMBL; CU329670; CAB11665.1; -; Genomic_DNA.
DR   PIR; T38317; T38317.
DR   RefSeq; NP_593395.1; NM_001018827.2.
DR   AlphaFoldDB; O13952; -.
DR   BioGRID; 278280; 4.
DR   STRING; 4896.SPAC23H4.11c.1; -.
DR   MaxQB; O13952; -.
DR   PaxDb; O13952; -.
DR   EnsemblFungi; SPAC23H4.11c.1; SPAC23H4.11c.1:pep; SPAC23H4.11c.
DR   GeneID; 2541788; -.
DR   KEGG; spo:SPAC23H4.11c; -.
DR   PomBase; SPAC23H4.11c; cnl2.
DR   VEuPathDB; FungiDB:SPAC23H4.11c; -.
DR   eggNOG; ENOG502R7FC; Eukaryota.
DR   HOGENOM; CLU_1441820_0_0_1; -.
DR   InParanoid; O13952; -.
DR   OMA; REQDMDR; -.
DR   PhylomeDB; O13952; -.
DR   PRO; PR:O13952; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0000776; C:kinetochore; IDA:PomBase.
DR   GO; GO:0031511; C:Mis6-Sim4 complex; IDA:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0007059; P:chromosome segregation; IBA:GO_Central.
DR   GO; GO:0000070; P:mitotic sister chromatid segregation; IC:PomBase.
DR   InterPro; IPR018565; Nkp2/Cnl2.
DR   PANTHER; PTHR28064; PTHR28064; 1.
DR   Pfam; PF09447; Cnl2_NKP2; 1.
PE   3: Inferred from homology;
KW   Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW   Cytoplasm; Kinetochore; Nucleus; Reference proteome.
FT   CHAIN           1..188
FT                   /note="Inner kinetochore subunit cnl2"
FT                   /id="PRO_0000351434"
SQ   SEQUENCE   188 AA;  21922 MW;  2B466F7F330D589F CRC64;
     MNMEEQILND YLLSQGRLQS IISLEQWRQL FPQRYREDPL IERLYEYCTQ QRQKRLAKLR
     ANIHLESQVI GKSRIDRMLA TNVEKLQTVS HASTLHDVEE FYTSHSAKPL DISEINERLS
     EAVQSAYTKL NEEKERCTQL TLKMNSQIAS LSDFQWSKEP NVDESIHLVE SLIESLEKAA
     PSAIEELD