NKRF_MOUSE
ID NKRF_MOUSE Reviewed; 690 AA.
AC Q8BY02; A2A3V7; Q8BJU5; Q9CRL2;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=NF-kappa-B-repressing factor;
DE Short=NFkB-repressing factor;
DE AltName: Full=Transcription factor NRF;
GN Name=Nkrf; Synonyms=Nrf;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, and Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Enhances the ATPase activity of DHX15 by acting like a brace
CC that tethers mobile sections of DHX15 together, stabilizing a
CC functional conformation with high RNA affinity of DHX15. Involved in
CC the constitutive silencing of the interferon beta promoter,
CC independently of the virus-induced signals, and in the inhibition of
CC the basal and cytokine-induced iNOS promoter activity. Also involved in
CC the regulation of IL-8 transcription. May also act as a DNA-binding
CC transcription regulator: interacts with a specific negative regulatory
CC element (NRE) 5'-AATTCCTCTGA-3' to mediate transcriptional repression
CC of certain NK-kappa-B responsive genes. {ECO:0000250|UniProtKB:O15226}.
CC -!- SUBUNIT: Interacts with NF-kappa-B. Interacts with XRN2. Interacts (via
CC G-patch domain) with DHX15; promoting the RNA helicase activity of
CC DHX15. {ECO:0000250|UniProtKB:O15226}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:O15226}.
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DR EMBL; AK020455; BAB32107.1; -; mRNA.
DR EMBL; AK042690; BAC31334.1; -; mRNA.
DR EMBL; AK079123; BAC37550.1; -; mRNA.
DR EMBL; AL450399; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC138828; AAI38829.1; -; mRNA.
DR EMBL; BC138829; AAI38830.1; -; mRNA.
DR RefSeq; NP_084167.2; NM_029891.2.
DR AlphaFoldDB; Q8BY02; -.
DR BioGRID; 218599; 6.
DR IntAct; Q8BY02; 2.
DR STRING; 10090.ENSMUSP00000061546; -.
DR PhosphoSitePlus; Q8BY02; -.
DR EPD; Q8BY02; -.
DR MaxQB; Q8BY02; -.
DR PaxDb; Q8BY02; -.
DR PeptideAtlas; Q8BY02; -.
DR PRIDE; Q8BY02; -.
DR ProteomicsDB; 293570; -.
DR Antibodypedia; 462; 211 antibodies from 30 providers.
DR DNASU; 77286; -.
DR Ensembl; ENSMUST00000057093; ENSMUSP00000061546; ENSMUSG00000044149.
DR GeneID; 77286; -.
DR KEGG; mmu:77286; -.
DR UCSC; uc009sxw.1; mouse.
DR CTD; 55922; -.
DR MGI; MGI:1924536; Nkrf.
DR VEuPathDB; HostDB:ENSMUSG00000044149; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000157256; -.
DR HOGENOM; CLU_025268_0_0_1; -.
DR InParanoid; Q8BY02; -.
DR OMA; FKHTYHE; -.
DR OrthoDB; 1179335at2759; -.
DR PhylomeDB; Q8BY02; -.
DR TreeFam; TF326321; -.
DR BioGRID-ORCS; 77286; 1 hit in 76 CRISPR screens.
DR ChiTaRS; Nkrf; mouse.
DR PRO; PR:Q8BY02; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; Q8BY02; protein.
DR Bgee; ENSMUSG00000044149; Expressed in pontine nuclear group and 223 other tissues.
DR ExpressionAtlas; Q8BY02; baseline and differential.
DR Genevisible; Q8BY02; MM.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0001671; F:ATPase activator activity; ISS:UniProtKB.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR CDD; cd02640; R3H_NRF; 1.
DR Gene3D; 3.30.1370.50; -; 1.
DR InterPro; IPR000467; G_patch_dom.
DR InterPro; IPR001374; R3H_dom.
DR InterPro; IPR036867; R3H_dom_sf.
DR InterPro; IPR034071; R3H_NRF.
DR Pfam; PF01585; G-patch; 1.
DR Pfam; PF01424; R3H; 1.
DR SMART; SM00443; G_patch; 1.
DR SMART; SM00393; R3H; 1.
DR SUPFAM; SSF82708; SSF82708; 1.
DR PROSITE; PS50174; G_PATCH; 1.
DR PROSITE; PS51061; R3H; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..690
FT /note="NF-kappa-B-repressing factor"
FT /id="PRO_0000096870"
FT DOMAIN 551..596
FT /note="G-patch"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00092"
FT DOMAIN 600..664
FT /note="R3H"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00382"
FT DNA_BIND 296..388
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT REGION 1..296
FT /note="Active repression domain"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT REGION 27..49
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 65..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 133..160
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 414..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 25..45
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT COMPBIAS 133..150
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..430
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 618
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT CROSSLNK 68
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT CROSSLNK 500
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT CROSSLNK 666
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT CROSSLNK 674
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:O15226"
FT CONFLICT 686
FT /note="M -> V (in Ref. 1; BAC37550)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 690 AA; 77739 MW; 0A99E1141183F999 CRC64;
MEKILHMAEG IDIGEMPSYD LMLPKPSKGQ KRYLSTYDGQ NPPKKQAGSK FHVRARFEPV
HFVASSSKAE RQEDPYGPQT KDVNGRTHFA SMPRNFYQDY TQDSFSIQDG NSQYCNSSGF
IFTKDQPVAT NMYFDSGNPA PSSTSQQANC QPAPEPPPSQ MYPESLVAEK QYFIEKLTAT
IWKNLSNPEM TSGSDKINYT YMLTRCIQAC KTNPEYIYAP LKEIPPADIP KNKKLLTDGY
ACEVRCQNIY LTTGYAGSKN GSRDRATELA VKLLQKRIEV RVVRRKFKHI IGEDLVVCQI
GMLSYEFPPA LKPPEDLVVL GKDASGQPIF NSSAKHWTNF VITENANDAI GILNNSASFN
KMSIEYKYEM MPNRTWRCRV FLQDHCLAEG YGTKKTSKHA AADEALKVLQ KTQPTYPSVK
SSQCHSGSSP KGSGKKKDIK DLVVYENSSN PVCTLNDTAQ FNRMTVEYVY ERMTGLRWKC
KVILESEVIA EAVGVKKSVK YEAAGEAVKT LKKTQPTVIN NLKKGTVEDV ISRNEIQGRS
AEEAYKQQIK EDNIGNQLLR KMGWTGGGLG KSGEGIREPI SVKEQHKREG LGLDVERVNK
IAKRDIEQII RNYARSESHS DLTFSTELTN DERKQIHQIA QKYGLKSKSH GVGHDRYLVV
GRKRRKEDLL DQLKQEGQVG HYELVMPQAN
