NKX12_MOUSE
ID NKX12_MOUSE Reviewed; 305 AA.
AC P42580; A4QPC3; Q9JHF2; Q9JKW5; Q9JKW6; Q9JKW9; Q9JKX0; Q9JKX1;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=NK1 transcription factor-related protein 2;
DE AltName: Full=Homeobox protein SAX-1;
DE AltName: Full=NKX-1.1;
GN Name=Nkx1-2; Synonyms=Nkx1.1, Sax1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=C57BL/6J;
RX PubMed=7669696; DOI=10.1016/0925-4773(95)00358-8;
RA Schubert F.R., Fainsod A., Gruenbaum Y., Gruss P.;
RT "Expression of the novel murine homeobox gene Sax-1 in the developing
RT nervous system.";
RL Mech. Dev. 51:99-114(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=10681422; DOI=10.1073/pnas.030539397;
RA Rovescalli A.C., Cinquanta M., Ferrante J., Kozak C.A., Nirenberg M.;
RT "The mouse Nkx-1.2 homeobox gene: alternative RNA splicing at canonical and
RT noncanonical splice sites.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:1982-1987(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 289-305.
RC STRAIN=BALB/cJ;
RA Hong S.B., Kim S.J., Noh M.J., Lee Y.M., Kim Y.S., Yoo O.J.;
RL Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP INTERACTION WITH HIPK1; HIPK2 AND HIPK3, AND PHOSPHORYLATION.
RC STRAIN=BALB/cJ;
RX PubMed=9748262; DOI=10.1074/jbc.273.40.25875;
RA Kim Y.H., Choi C.Y., Lee S.-J., Conti M.A., Kim Y.;
RT "Homeodomain-interacting protein kinases, a novel family of co-repressors
RT for homeodomain transcription factors.";
RL J. Biol. Chem. 273:25875-25879(1998).
CC -!- FUNCTION: May function in cell specification, particularly in the CNS.
CC -!- SUBUNIT: Interacts with HIPK1, HIPK2, and HIPK3.
CC {ECO:0000269|PubMed:9748262}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expression detected in adult brain, testis and
CC spleen. In the testis, expressed in the germ cells of the seminiferous
CC epithelium, in elongating spermatids and in spermatozoa. Expressed
CC throughout the brain with highest levels in regions of the cerebral
CC cortex, hippocampus, diencephalon, pons, medulla and cerebellum. In the
CC embryo, expressed in the developing posterior central nervous system.
CC First seen in the ectoderm lateral to the primitive streak, later it
CC encompasses the neural plate. Starting at day 9.5 pc, expressed in
CC distinct areas of spinal cord, hindbrain, midbrain and forebrain.
CC {ECO:0000269|PubMed:10681422, ECO:0000269|PubMed:7669696}.
CC -!- DEVELOPMENTAL STAGE: In the embryo, expressed at highest levels at day
CC 10 with levels decreasing during further development.
CC {ECO:0000269|PubMed:10681422}.
CC -!- PTM: Phosphorylated by HIPK2 in vitro. {ECO:0000269|PubMed:9748262}.
CC -!- SIMILARITY: Belongs to the NK-1 homeobox family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF43670.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAF43672.1; Type=Miscellaneous discrepancy; Note=Probable cloning artifact.; Evidence={ECO:0000305};
CC Sequence=AAF43674.1; Type=Miscellaneous discrepancy; Note=Aberrant splicing.; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X75384; CAA53153.1; -; mRNA.
DR EMBL; AF222443; AAF43669.1; -; Genomic_DNA.
DR EMBL; AF222443; AAF43670.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AF222444; AAF43671.1; -; mRNA.
DR EMBL; AF222445; AAF43672.1; ALT_SEQ; mRNA.
DR EMBL; AF223361; AAF43673.1; -; mRNA.
DR EMBL; AF223362; AAF43674.1; ALT_SEQ; mRNA.
DR EMBL; BC139757; AAI39758.1; -; mRNA.
DR EMBL; U58137; AAB06948.1; -; Genomic_DNA.
DR CCDS; CCDS21924.1; -.
DR PIR; S41860; S41860.
DR RefSeq; NP_033149.1; NM_009123.2.
DR AlphaFoldDB; P42580; -.
DR SMR; P42580; -.
DR STRING; 10090.ENSMUSP00000052778; -.
DR PaxDb; P42580; -.
DR PRIDE; P42580; -.
DR Antibodypedia; 60104; 83 antibodies from 17 providers.
DR DNASU; 20231; -.
DR Ensembl; ENSMUST00000054562; ENSMUSP00000052778; ENSMUSG00000048528.
DR GeneID; 20231; -.
DR KEGG; mmu:20231; -.
DR UCSC; uc009kcc.1; mouse.
DR CTD; 390010; -.
DR MGI; MGI:104806; Nkx1-2.
DR VEuPathDB; HostDB:ENSMUSG00000048528; -.
DR eggNOG; KOG0488; Eukaryota.
DR GeneTree; ENSGT00940000162535; -.
DR HOGENOM; CLU_063378_0_0_1; -.
DR InParanoid; P42580; -.
DR OMA; EPSCAKP; -.
DR OrthoDB; 1371237at2759; -.
DR PhylomeDB; P42580; -.
DR TreeFam; TF316128; -.
DR BioGRID-ORCS; 20231; 3 hits in 73 CRISPR screens.
DR ChiTaRS; Nkx1-2; mouse.
DR PRO; PR:P42580; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P42580; protein.
DR Bgee; ENSMUSG00000048528; Expressed in primitive streak and 46 other tissues.
DR Genevisible; P42580; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT CHAIN 1..305
FT /note="NK1 transcription factor-related protein 2"
FT /id="PRO_0000049290"
FT DNA_BIND 156..215
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 51..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..257
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..112
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 216
FT /note="P -> S (in Ref. 2; AAF43673)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 305 AA; 32013 MW; E02E09A40453FF1B CRC64;
MLAWQDVGAK AAPSHHKISF SVLDILDPQK FTRAALPPVR LAALEAKKSL EEVEAGQDAC
SGNPIGSQET PDAVGRGIDP GSPVEGSEAE EEEEAEDAGR AHQPERWQGV HEGSPEARAV
AVGTEESGAE GLPASPGSPG SPRPRRRRAE SSCAKPRRAR TAFTYEQLVA LENKFRATRY
LSVCERLNLA LSLSLTETQV KIWFQNRRTK WKKQNPGADG AVQAGGGAPQ PGTPGAVAGG
GGSATGSSPG PPVPGALPYQ TFPTYPATNV LFPAASFPLT TAANGSPFTP FLGPSYLTPF
YAPHL
