NKX23_MOUSE
ID NKX23_MOUSE Reviewed; 362 AA.
AC P97334; Q9QZ60; Q9WV67;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Homeobox protein Nkx-2.3;
DE AltName: Full=Homeobox protein NK-2 homolog 3;
DE AltName: Full=Homeobox protein NK-2 homolog C;
DE AltName: Full=Nkx2-C;
GN Name=Nkx2-3; Synonyms=Nkx-2.3, Nkx2c;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=129; TISSUE=Liver;
RX PubMed=9142493;
RX DOI=10.1002/(sici)1097-0177(199705)209:1<29::aid-aja3>3.0.co;2-z;
RA Pabst O., Schneider A., Brand T., Arnold H.-H.;
RT "The mouse Nkx2-3 homeodomain gene is expressed in gut mesenchyme during
RT pre- and postnatal mouse development.";
RL Dev. Dyn. 209:29-35(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=129;
RA Wang C.-C., Biben C., Robb R., Tarlinton D., Nassir F., Davidson N.O.,
RA Harvey R.P.;
RT "Homeodomain factor Nkx2-3 is required for normal development of the gut-
RT associated lymphoid tissue and the spleen.";
RL Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=BALB/cJ;
RX PubMed=10926756; DOI=10.1006/dbio.2000.9749;
RA Wang C.-C., Biben C., Robb L., Nassir F., Barnett L., Davidson N.O.,
RA Koentgen F., Tarlinton D., Harvey R.P.;
RT "Homeodomain factor Nkx2-3 controls regional expression of leukocyte homing
RT coreceptor MAdCAM-1 in specialized endothelial cells of the viscera.";
RL Dev. Biol. 224:152-167(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION.
RX PubMed=10790368; DOI=10.1093/emboj/19.9.2015;
RA Pabst O., Foerster R., Lipp M., Engel H., Arnold H.-H.;
RT "NKX2.3 is required for MAdCAM-1 expression and homing of lymphocytes in
RT spleen and mucosa-associated lymphoid tissue.";
RL EMBO J. 19:2015-2023(2000).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=12141427;
RA Biben C., Wang C.-C., Harvey R.P.;
RT "NK-2 class homeobox genes and pharyngeal/oral patterning: Nkx2-3 is
RT required for salivary gland and tooth morphogenesis.";
RL Int. J. Dev. Biol. 46:415-422(2002).
CC -!- FUNCTION: Transcriptional regulator essential for normal development
CC and functions of the small intestine and spleen. Activates directly
CC MADCAM1 expression. Required for homing of lymphocytes in spleen and
CC mucosa-associated lymphoid tissue. May have a role during pharyngeal
CC organogenesis. {ECO:0000269|PubMed:10790368}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in spleen and intestine. Also expressed
CC in salivary gland and tongue. {ECO:0000269|PubMed:12141427}.
CC -!- DEVELOPMENTAL STAGE: Expressed in gut mesenchyme during pre- and
CC postnatal development. Expressed as well in the pharyngeal floor and
CC pouches, and in the oral and branchial arch ectoderm. Expression
CC persisted in the developing thyroid until birth, in mucous forming
CC cells of salivary glands and in odontogenic epithelium of the mandible.
CC -!- SIMILARITY: Belongs to the NK-2 homeobox family. {ECO:0000305}.
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DR EMBL; Y11117; CAA72002.1; -; mRNA.
DR EMBL; AF202036; AAF08008.1; -; mRNA.
DR EMBL; AF155583; AAD38415.1; -; Genomic_DNA.
DR EMBL; BC072614; AAH72614.1; -; mRNA.
DR CCDS; CCDS29833.1; -.
DR RefSeq; NP_032725.1; NM_008699.2.
DR AlphaFoldDB; P97334; -.
DR SMR; P97334; -.
DR BioGRID; 201777; 2.
DR IntAct; P97334; 2.
DR STRING; 10090.ENSMUSP00000050933; -.
DR iPTMnet; P97334; -.
DR PhosphoSitePlus; P97334; -.
DR PaxDb; P97334; -.
DR PRIDE; P97334; -.
DR ProteomicsDB; 293571; -.
DR Antibodypedia; 31083; 96 antibodies from 25 providers.
DR DNASU; 18089; -.
DR Ensembl; ENSMUST00000057178; ENSMUSP00000050933; ENSMUSG00000044220.
DR GeneID; 18089; -.
DR KEGG; mmu:18089; -.
DR UCSC; uc008hok.1; mouse.
DR CTD; 159296; -.
DR MGI; MGI:97348; Nkx2-3.
DR VEuPathDB; HostDB:ENSMUSG00000044220; -.
DR eggNOG; KOG0842; Eukaryota.
DR GeneTree; ENSGT00940000157556; -.
DR HOGENOM; CLU_049543_0_0_1; -.
DR InParanoid; P97334; -.
DR OMA; QKSCPLK; -.
DR OrthoDB; 1191455at2759; -.
DR PhylomeDB; P97334; -.
DR TreeFam; TF351204; -.
DR BioGRID-ORCS; 18089; 0 hits in 74 CRISPR screens.
DR ChiTaRS; Nkx2-3; mouse.
DR PRO; PR:P97334; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; P97334; protein.
DR Bgee; ENSMUSG00000044220; Expressed in hindgut and 47 other tissues.
DR Genevisible; P97334; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030183; P:B cell differentiation; IMP:MGI.
DR GO; GO:0043367; P:CD4-positive, alpha-beta T cell differentiation; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0048565; P:digestive tract development; IMP:MGI.
DR GO; GO:0022612; P:gland morphogenesis; IMP:MGI.
DR GO; GO:0006955; P:immune response; IMP:MGI.
DR GO; GO:0001776; P:leukocyte homeostasis; IMP:MGI.
DR GO; GO:0050900; P:leukocyte migration; IMP:MGI.
DR GO; GO:0048535; P:lymph node development; IMP:MGI.
DR GO; GO:0030225; P:macrophage differentiation; IMP:MGI.
DR GO; GO:0048537; P:mucosa-associated lymphoid tissue development; IMP:MGI.
DR GO; GO:0042475; P:odontogenesis of dentin-containing tooth; IMP:MGI.
DR GO; GO:0048541; P:Peyer's patch development; IMP:MGI.
DR GO; GO:0002317; P:plasma cell differentiation; IMP:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:MGI.
DR GO; GO:0009791; P:post-embryonic development; IMP:MGI.
DR GO; GO:0048621; P:post-embryonic digestive tract morphogenesis; IMP:MGI.
DR GO; GO:0050678; P:regulation of epithelial cell proliferation; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0048536; P:spleen development; IMP:MGI.
DR GO; GO:0006366; P:transcription by RNA polymerase II; IMP:MGI.
DR GO; GO:0006641; P:triglyceride metabolic process; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..362
FT /note="Homeobox protein Nkx-2.3"
FT /id="PRO_0000048934"
FT DNA_BIND 145..204
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 126..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 203..222
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 128..143
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 44
FT /note="C -> R (in Ref. 1; CAA72002)"
FT /evidence="ECO:0000305"
FT CONFLICT 61
FT /note="D -> E (in Ref. 1; CAA72002)"
FT /evidence="ECO:0000305"
FT CONFLICT 132
FT /note="K -> R (in Ref. 1; CAA72002)"
FT /evidence="ECO:0000305"
FT CONFLICT 248..250
FT /note="GVG -> RC (in Ref. 1; CAA72002)"
FT /evidence="ECO:0000305"
FT CONFLICT 261..357
FT /note="AYGYGNSAAAAAAAAAAAAAAAAYSGSYGCAYPTGGGGGGGGTASAATTAMQ
FT PACSATGGGSFVNVSNLGGFGSGGGAQPLHQGAAAGSACTQGTLQ -> TATGTRPPQP
FT LQPPQPQQQGLQRQLRLAYRPVAAVVVAARPPRRPPPCNPPAAPPGDLRVTAGIEAFVP
FT LVR (in Ref. 1; CAA72002)"
FT /evidence="ECO:0000305"
FT CONFLICT 349
FT /note="S -> A (in Ref. 3; AAD38415)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 362 AA; 38090 MW; 48886528EC3E1622 CRC64;
MMLPSPVTST PFSVKDILNL EQQRHFHGAH LQAELEQHFH SAPCMLATAE GTQFSDAGEE
DEEEEGEKLS YLNSLAAAEG HGDSGLCPQS YVHTVLRDAC SGPKEQEEEV VSERSQKSCQ
LKKSLEAAGD CKTSEDGERP KPRSRRKPRV LFSQAQVFEL ERRFKQQRYL SAPEREHLAS
SLKLTSTQVK IWFQNRRYKC KRQRQDKSLE LGTHAPPPPP RRVAVPVLVR DGKPCVTPSA
QTYGSPYGVG AGAYSYNSFP AYGYGNSAAA AAAAAAAAAA AAAYSGSYGC AYPTGGGGGG
GGTASAATTA MQPACSATGG GSFVNVSNLG GFGSGGGAQP LHQGAAAGSA CTQGTLQGIR
AW
