ID   NKX25_XENLA             Reviewed;         299 AA.
AC   P42583;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 112.
DE   RecName: Full=Homeobox protein Nkx-2.5;
DE            Short=xNKx-2.5;
DE   AltName: Full=Homeobox protein NK-2 homolog E;
GN   Name=nkx-2.5;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Heart;
RX   PubMed=7545912; DOI=10.1006/dbio.1994.1089;
RA   Tonissen K.F., Drysdale T.A., Lints T.J., Harvey R.P., Krieg P.A.;
RT   "XNkx-2.5, a Xenopus gene related to Nkx-2.5 and tinman: evidence for a
RT   conserved role in cardiac development.";
RL   Dev. Biol. 162:325-328(1994).
CC   -!- FUNCTION: Transcription factor required for the development of the
CC       heart and the spleen. Implicated in commitment to and/or
CC       differentiation of the myocardial lineage. May regulate the expression
CC       of genes involved in cardiogenesis and play a role in the formation of
CC       gut and the pharyngeal region. Binds to the core DNA motif of promoter.
CC       {ECO:0000250|UniProtKB:P42582, ECO:0000250|UniProtKB:P52952}.
CC   -!- SUBUNIT: Homodimer (via the homeobox); binds DNA as homodimer.
CC       {ECO:0000250|UniProtKB:P52952}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Heart and gut tissue.
CC   -!- DEVELOPMENTAL STAGE: It is first expressed in early neurula embryos
CC       (about stage 15) in a bi-lobed pattern at the front of the embryo (this
CC       corresponds to the presumptive heart mesoderm). By the end of neural
CC       fold closure it is highly expressed in the presumptive heart region. By
CC       stage 26-27 (at which heart differentiation begins) its expression can
CC       be clearly seen in the heart and the level in embryonic heart decreases
CC       at later stages of development. As expression within heart declines low
CC       level expression is seen in the foregut and the pharyngeal region.
CC   -!- DOMAIN: The homeobox domain binds to double-stranded DNA.
CC       {ECO:0000250|UniProtKB:P52952}.
CC   -!- SIMILARITY: Belongs to the NK-2 homeobox family. {ECO:0000305}.
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DR   EMBL; L25600; AAA19861.1; -; mRNA.
DR   PIR; I51442; I51442.
DR   AlphaFoldDB; P42583; -.
DR   SMR; P42583; -.
DR   Proteomes; UP000186698; Genome assembly.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR   GO; GO:0032993; C:protein-DNA complex; ISS:UniProtKB.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISS:UniProtKB.
DR   GO; GO:0060048; P:cardiac muscle contraction; IEA:InterPro.
DR   GO; GO:0007507; P:heart development; IEA:InterPro.
DR   GO; GO:1903779; P:regulation of cardiac conduction; IEA:InterPro.
DR   CDD; cd00086; homeodomain; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR033629; NKX-2.5.
DR   PANTHER; PTHR24340:SF28; PTHR24340:SF28; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome.
FT   CHAIN           1..299
FT                   /note="Homeobox protein Nkx-2.5"
FT                   /id="PRO_0000048941"
FT   DNA_BIND        124..183
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000250|UniProtKB:P52952,
FT                   ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          90..128
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        90..121
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   299 AA;  34097 MW;  058A345B9E1D76ED CRC64;
     MFASPVTSTP FSVKDILNLE QHQSGLSPMD ITSRLENSSC MLSTFKQESY PGTPCLSELT
     EEMSQRDTAK GPSSFPGSFF VKNYLEMDSK DPKDHKKDIC PLQKTLEHDK REAEDPERPR
     QRKRRKPRVL FSQAQVYELE RRFKQQKYLS APERDHLANV LKLTSTQVKI WFQNRRYKCK
     RQRQDQTLEM VGLPPPRRIA VPVLVRDGKP CLGESSPYNS PYNVSINPYS YNTYPAYSNY
     SNPACSGSYN CSYSSMPSMQ PTSAGNNFMN FSVGDLNTVQ TPIQQASSVS ALHHGIRAW