NKX32_MOUSE
ID NKX32_MOUSE Reviewed; 333 AA.
AC P97503; A6H6G9;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Homeobox protein Nkx-3.2;
DE AltName: Full=Bagpipe homeobox protein homolog 1;
DE AltName: Full=Homeobox protein NK-3 homolog B;
GN Name=Nkx3-2; Synonyms=Bapx1, Nkx-3.2, Nkx3b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9256352; DOI=10.1016/s0925-4773(97)00067-1;
RA Tribioli C., Frasch M., Lufkin T.;
RT "Bapx1: an evolutionary conserved homologue of the Drosophila bagpipe
RT homeobox gene is expressed in splanchnic mesoderm and the embryonic
RT skeleton.";
RL Mech. Dev. 65:145-162(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=14973294; DOI=10.1242/dev.01017;
RA Tucker A.S., Watson R.P., Lettice L.A., Yamada G., Hill R.E.;
RT "Bapx1 regulates patterning in the middle ear: altered regulatory role in
RT the transition from the proximal jaw during vertebrate evolution.";
RL Development 131:1235-1245(2004).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16421188; DOI=10.1242/dev.02258;
RA Provot S., Kempf H., Murtaugh L.C., Chung U.I., Kim D.W., Chyung J.,
RA Kronenberg H.M., Lassar A.B.;
RT "Nkx3.2/Bapx1 acts as a negative regulator of chondrocyte maturation.";
RL Development 133:651-662(2006).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=19208343; DOI=10.1053/j.gastro.2009.01.009;
RA Verzi M.P., Stanfel M.N., Moses K.A., Kim B.M., Zhang Y., Schwartz R.J.,
RA Shivdasani R.A., Zimmer W.E.;
RT "Role of the homeodomain transcription factor Bapx1 in mouse distal stomach
RT development.";
RL Gastroenterology 136:1701-1710(2009).
CC -!- FUNCTION: Transcriptional repressor that acts as a negative regulator
CC of chondrocyte maturation. PLays a role in distal stomach development;
CC required for proper antral-pyloric morphogenesis and development of
CC antral-type epithelium. In concert with GSC, defines the structural
CC components of the middle ear; required for tympanic ring and gonium
CC development and in the regulation of the width of the malleus.
CC {ECO:0000269|PubMed:14973294, ECO:0000269|PubMed:16421188,
CC ECO:0000269|PubMed:19208343}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed widely in mesoderm at the gastroduodenal
CC junction (at protein level). Expressed in visceral mesoderm and
CC embryonic skeleton. Expression is restricted to immature proliferative
CC chondrocytes during endochondral ossification.
CC {ECO:0000269|PubMed:16421188, ECO:0000269|PubMed:19208343}.
CC -!- DEVELOPMENTAL STAGE: During embryogenesis, expressed in a discrete
CC domain within the mandibular component of the first branchial arch and
CC later in the primordia of middle earassociated bones, the gonium and
CC tympanic ring. {ECO:0000269|PubMed:14973294}.
CC -!- SIMILARITY: Belongs to the NK-3 homeobox family. {ECO:0000305}.
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DR EMBL; U87957; AAB47764.1; -; mRNA.
DR EMBL; CH466524; EDL37578.1; -; Genomic_DNA.
DR EMBL; BC145872; AAI45873.1; -; mRNA.
DR EMBL; BC145874; AAI45875.1; -; mRNA.
DR CCDS; CCDS19259.1; -.
DR RefSeq; NP_031550.2; NM_007524.3.
DR AlphaFoldDB; P97503; -.
DR SMR; P97503; -.
DR BioGRID; 198300; 3.
DR CORUM; P97503; -.
DR STRING; 10090.ENSMUSP00000051990; -.
DR iPTMnet; P97503; -.
DR PhosphoSitePlus; P97503; -.
DR EPD; P97503; -.
DR MaxQB; P97503; -.
DR PaxDb; P97503; -.
DR PeptideAtlas; P97503; -.
DR PRIDE; P97503; -.
DR ProteomicsDB; 252968; -.
DR Antibodypedia; 22925; 208 antibodies from 30 providers.
DR DNASU; 12020; -.
DR Ensembl; ENSMUST00000060820; ENSMUSP00000051990; ENSMUSG00000049691.
DR GeneID; 12020; -.
DR KEGG; mmu:12020; -.
DR UCSC; uc008xhc.1; mouse.
DR CTD; 579; -.
DR MGI; MGI:108015; Nkx3-2.
DR VEuPathDB; HostDB:ENSMUSG00000049691; -.
DR eggNOG; KOG0842; Eukaryota.
DR GeneTree; ENSGT00940000161843; -.
DR HOGENOM; CLU_049543_2_0_1; -.
DR InParanoid; P97503; -.
DR OMA; SEACEPT; -.
DR OrthoDB; 1303626at2759; -.
DR TreeFam; TF351204; -.
DR BioGRID-ORCS; 12020; 4 hits in 73 CRISPR screens.
DR ChiTaRS; Nkx3-2; mouse.
DR PRO; PR:P97503; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; P97503; protein.
DR Bgee; ENSMUSG00000049691; Expressed in gut mesentery and 76 other tissues.
DR Genevisible; P97503; MM.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0048513; P:animal organ development; IMP:MGI.
DR GO; GO:0048645; P:animal organ formation; IMP:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0007368; P:determination of left/right symmetry; IMP:MGI.
DR GO; GO:0055123; P:digestive system development; IMP:MGI.
DR GO; GO:0048706; P:embryonic skeletal system development; IMP:MGI.
DR GO; GO:0060576; P:intestinal epithelial cell development; IGI:MGI.
DR GO; GO:0042474; P:middle ear morphogenesis; IMP:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:MGI.
DR GO; GO:0032331; P:negative regulation of chondrocyte differentiation; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0031016; P:pancreas development; IMP:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0001501; P:skeletal system development; IMP:MGI.
DR GO; GO:0048705; P:skeletal system morphogenesis; IMP:MGI.
DR GO; GO:0048536; P:spleen development; IMP:MGI.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..333
FT /note="Homeobox protein Nkx-3.2"
FT /id="PRO_0000048948"
FT DNA_BIND 206..265
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 74..121
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 137..212
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 76..91
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 134
FT /note="A -> P (in Ref. 1; AAB47764)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="S -> T (in Ref. 1; AAB47764)"
FT /evidence="ECO:0000305"
FT CONFLICT 292
FT /note="Q -> L (in Ref. 1; AAB47764)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 333 AA; 35167 MW; D089523714C86BA6 CRC64;
MAVRGSGTLT PFSIQAILNK KEERGGLATP EGRPAPGGTE VAVTAAPAVC CWRIFGETEA
GALGGAEDSL LASPARTRTA VGQSAESPGG WDSDSALSEE NEGRRRCADV PGASGTGRAR
VTLGLDQPGC ELHAAKDLEE EAPVRSDSEM SASVSGDHSP RGEDDSVSPG GARVPGLRGA
AGSGASGGQA GGVEEEEEPA APKPRKKRSR AAFSHAQVFE LERRFNHQRY LSGPERADLA
ASLKLTETQV KIWFQNRRYK TKRRQMAADL LASAPAAKKV AVKVLVRDDQ RQYLPGEVLR
PPSLLPLQPS YYYPYYCLPG WALSTCAAAA GTQ
