NKX63_MOUSE
ID NKX63_MOUSE Reviewed; 262 AA.
AC Q3UHX8; Q4JGN0; Q9D2W8;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=Homeobox protein Nkx-6.3;
GN Name=Nkx6-3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J;
RX PubMed=16326147; DOI=10.1016/j.modgep.2005.06.012;
RA Alanentalo T., Chatonnet F., Karlen M., Sulniute R., Ericson J.,
RA Andersson E., Ahlgren U.;
RT "Cloning and analysis of Nkx6.3 during CNS and gastrointestinal
RT development.";
RL Gene Expr. Patterns 6:162-170(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Cecum, and Stomach;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP DEVELOPMENTAL STAGE.
RX PubMed=16297379; DOI=10.1016/j.ydbio.2005.10.001;
RG The Beta Cell Biology Consortium;
RA Pedersen J.K., Nelson S.B., Jorgensen M.C., Henseleit K.D., Fujitani Y.,
RA Wright C.V.E., Sander M., Serup P.;
RT "Endodermal expression of Nkx6 genes depends differentially on Pdx1.";
RL Dev. Biol. 288:487-501(2005).
RN [4]
RP DEVELOPMENTAL STAGE.
RX PubMed=15928328; DOI=10.1369/jhc.5b6619.2005;
RA Nelson S.B., Janiesch C., Sander M.;
RT "Expression of Nkx6 genes in the hindbrain and gut of the developing
RT mouse.";
RL J. Histochem. Cytochem. 53:787-790(2005).
CC -!- FUNCTION: Putative transcription factor, which may be involved in
CC patterning of central nervous system and pancreas. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing CNS and gastro-
CC intestinal tract. {ECO:0000269|PubMed:16326147}.
CC -!- DEVELOPMENTAL STAGE: In the developing embryo, it is confined to the
CC gut and caudal hindbrain. In caudal hindbrain, it is specifically
CC expressed in a subpopulation of differentiating V2 neurons. Not
CC detected prior to 12.5 dpc and is not found in motor nuclei. At 12.5
CC dpc, it is expressed in the ventral medial aspect of the medullary
CC reticular formation (MDR). At 16.5 dpc, expression is confined to the
CC gigantocellular nucleus, a nucleus that is part of the MDR. In addition
CC to the CNS, it is also expressed in the developing gut, in duodenal and
CC glandular stomach endoderm and, at the end of gestation becomes
CC restricted to the base of the gastric units in the glandular stomach.
CC Expressed at very low level in the pancreatic epithelium. Expressed in
CC the pancreas at 15.5 dpc. {ECO:0000269|PubMed:15928328,
CC ECO:0000269|PubMed:16297379, ECO:0000269|PubMed:16326147}.
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DR EMBL; DQ093570; AAY98485.1; -; mRNA.
DR EMBL; AK018683; BAB31342.1; -; mRNA.
DR EMBL; AK147163; BAE27728.1; -; mRNA.
DR CCDS; CCDS52524.1; -.
DR RefSeq; NP_083278.1; NM_029002.2.
DR AlphaFoldDB; Q3UHX8; -.
DR SMR; Q3UHX8; -.
DR BioGRID; 216845; 3.
DR STRING; 10090.ENSMUSP00000071517; -.
DR iPTMnet; Q3UHX8; -.
DR PhosphoSitePlus; Q3UHX8; -.
DR PaxDb; Q3UHX8; -.
DR PRIDE; Q3UHX8; -.
DR ProteomicsDB; 252903; -.
DR Antibodypedia; 23941; 144 antibodies from 22 providers.
DR Ensembl; ENSMUST00000071588; ENSMUSP00000071517; ENSMUSG00000063672.
DR GeneID; 74561; -.
DR KEGG; mmu:74561; -.
DR UCSC; uc009len.1; mouse.
DR CTD; 157848; -.
DR MGI; MGI:1921811; Nkx6-3.
DR VEuPathDB; HostDB:ENSMUSG00000063672; -.
DR eggNOG; KOG0847; Eukaryota.
DR GeneTree; ENSGT00940000160545; -.
DR HOGENOM; CLU_064820_1_1_1; -.
DR InParanoid; Q3UHX8; -.
DR OMA; GPQVGNF; -.
DR OrthoDB; 1263401at2759; -.
DR PhylomeDB; Q3UHX8; -.
DR TreeFam; TF327063; -.
DR BioGRID-ORCS; 74561; 0 hits in 72 CRISPR screens.
DR PRO; PR:Q3UHX8; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q3UHX8; protein.
DR Bgee; ENSMUSG00000063672; Expressed in duodenum and 15 other tissues.
DR ExpressionAtlas; Q3UHX8; baseline and differential.
DR Genevisible; Q3UHX8; MM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; ISO:MGI.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0043565; F:sequence-specific DNA binding; ISO:MGI.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0001709; P:cell fate determination; IMP:MGI.
DR GO; GO:0035883; P:enteroendocrine cell differentiation; IMP:MGI.
DR GO; GO:0030857; P:negative regulation of epithelial cell differentiation; IMP:MGI.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..262
FT /note="Homeobox protein Nkx-6.3"
FT /id="PRO_0000311331"
FT DNA_BIND 140..199
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 197..237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..237
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 127
FT /note="S -> G (in Ref. 1; AAY98485)"
FT /evidence="ECO:0000305"
FT CONFLICT 238
FT /note="D -> N (in Ref. 2; BAB31342)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 262 AA; 28779 MW; BE45E53D60DF10B6 CRC64;
MESNLQGTFL LNNTQLAQFS EMKAPMCQYS VQNSFYKLSP PGLGPQLAAG TPHGITDILS
RPVATPNSSL LSGYPHVAGF GGLSSQGVYY GPQVGSFSKA GNEYPTRTRN CWADTGQDWR
GSARPCSNTP DPLSDTIHKK KHTRPTFTGH QIFALEKTFE QTKYLAGPER ARLAYSLGMT
ESQVKVWFQN RRTKWRKKSA LEPSSSTPRA PGGASGDRAA SENEDDEYNK PLDPDSDDEK
IRLLLRKHRA AFSVLSLGAH SV
