NLE1_ARATH
ID NLE1_ARATH Reviewed; 473 AA.
AC Q9FLX9;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 124.
DE RecName: Full=Notchless protein homolog {ECO:0000303|PubMed:23572950};
GN Name=NLE1 {ECO:0000303|PubMed:23572950};
GN OrderedLocusNames=At5g52820 {ECO:0000312|Araport:AT5G52820};
GN ORFNames=MXC20.4 {ECO:0000312|EMBL:BAB10430.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9628582; DOI=10.1093/dnares/5.1.41;
RA Sato S., Kaneko T., Kotani H., Nakamura Y., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. IV. Sequence
RT features of the regions of 1,456,315 bp covered by nineteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:41-54(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP DWD MOTIF.
RX PubMed=18223036; DOI=10.1105/tpc.107.055418;
RA Lee J.H., Terzaghi W., Gusmaroli G., Charron J.B., Yoon H.J., Chen H.,
RA He Y.J., Xiong Y., Deng X.W.;
RT "Characterization of Arabidopsis and rice DWD proteins and their roles as
RT substrate receptors for CUL4-RING E3 ubiquitin ligases.";
RL Plant Cell 20:152-167(2008).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Wassilewskija;
RX PubMed=23572950; DOI=10.1007/s12298-010-0005-y;
RA Chantha S.-C., Gray-Mitsumune M., Houde J., Matton D.P.;
RT "The MIDASIN and NOTCHLESS genes are essential for female gametophyte
RT development in Arabidopsis thaliana.";
RL Physiol. Mol. Biol. Plants 16:3-18(2010).
CC -!- FUNCTION: Required for female gametophyte development.
CC {ECO:0000269|PubMed:23572950}.
CC -!- SUBUNIT: Associates with the pre-60S ribosomal particle.
CC {ECO:0000250|UniProtKB:P25382}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:P25382}.
CC -!- TISSUE SPECIFICITY: Constitutively and ubiquitously expressed.
CC {ECO:0000269|PubMed:23572950}.
CC -!- DOMAIN: The DWD box is required for interaction with DDB1A.
CC {ECO:0000305|PubMed:18223036}.
CC -!- DISRUPTION PHENOTYPE: Female semisterility due to strongly delayed
CC development of female gametophyte. {ECO:0000269|PubMed:23572950}.
CC -!- SIMILARITY: Belongs to the NLE1/RSA4 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AB009055; BAB10430.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96265.1; -; Genomic_DNA.
DR EMBL; AK227330; BAE99344.1; -; mRNA.
DR EMBL; BT004562; AAO42808.1; -; mRNA.
DR RefSeq; NP_200094.1; NM_124660.4.
DR AlphaFoldDB; Q9FLX9; -.
DR SMR; Q9FLX9; -.
DR STRING; 3702.AT5G52820.1; -.
DR PaxDb; Q9FLX9; -.
DR PRIDE; Q9FLX9; -.
DR ProteomicsDB; 251140; -.
DR EnsemblPlants; AT5G52820.1; AT5G52820.1; AT5G52820.
DR GeneID; 835359; -.
DR Gramene; AT5G52820.1; AT5G52820.1; AT5G52820.
DR KEGG; ath:AT5G52820; -.
DR Araport; AT5G52820; -.
DR TAIR; locus:2176897; AT5G52820.
DR eggNOG; KOG0271; Eukaryota.
DR HOGENOM; CLU_000288_57_16_1; -.
DR InParanoid; Q9FLX9; -.
DR OMA; HINCLAW; -.
DR OrthoDB; 723347at2759; -.
DR PhylomeDB; Q9FLX9; -.
DR PRO; PR:Q9FLX9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FLX9; baseline and differential.
DR Genevisible; Q9FLX9; AT.
DR GO; GO:0080008; C:Cul4-RING E3 ubiquitin ligase complex; ISS:TAIR.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0009553; P:embryo sac development; IMP:TAIR.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR012972; NLE.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08154; NLE; 1.
DR Pfam; PF00400; WD40; 8.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 4.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 2: Evidence at transcript level;
KW Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..473
FT /note="Notchless protein homolog"
FT /id="PRO_0000284893"
FT REPEAT 107..146
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 149..188
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 192..236
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 239..277
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 313..354
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 358..399
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 400..439
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 442..473
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REGION 9..91
FT /note="Ubiquitin-like (UBL) domain"
FT /evidence="ECO:0000250|UniProtKB:P25382"
FT MOTIF 417..432
FT /note="DWD box"
FT /evidence="ECO:0000305|PubMed:18223036"
SQ SEQUENCE 473 AA; 52897 MW; 8D5AAC83BE9EBFE4 CRC64;
MTMMDTDEGK TVMCLLTDPE GTHLGSAMYI PQKAGPLQLT QLVNRFLDNE EMLPYSFYVS
DEELLVPVGT YLEKNKVSVE KVLTIVYQQQ AVFRIRPVNR CSQTIAGHAE AVLCVSFSPD
GKQLASGSGD TTVRLWDLYT ETPLFTCKGH KNWVLTVAWS PDGKHLVSGS KSGEICCWNP
KKGELEGSPL TGHKKWITGI SWEPVHLSSP CRRFVTSSKD GDARIWDITL KKSIICLSGH
TLAVTCVKWG GDGIIYTGSQ DCTIKMWETT QGKLIRELKG HGHWINSLAL STEYVLRTGA
FDHTGRQYPP NEEKQKALER YNKTKGDSPE RLVSGSDDFT MFLWEPSVSK QPKKRLTGHQ
QLVNHVYFSP DGKWIASASF DKSVRLWNGI TGQFVTVFRG HVGPVYQVSW SADSRLLLSG
SKDSTLKIWE IRTKKLKQDL PGHADEVFAV DWSPDGEKVV SGGKDRVLKL WKG
