ID   NLE1_BOVIN              Reviewed;         486 AA.
AC   Q58D20;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   06-MAR-2013, sequence version 3.
DT   03-AUG-2022, entry version 115.
DE   RecName: Full=Notchless protein homolog 1;
GN   Name=NLE1;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
CC   -!- FUNCTION: Plays a role in regulating Notch activity. Plays a role in
CC       regulating the expression of CDKN1A and several members of the Wnt
CC       pathway, probably via its effects on Notch activity. Required during
CC       embryogenesis for inner mass cell survival (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Associates with the pre-60S ribosomal particle. Interacts (via
CC       WD repeats) with uL18 (By similarity). Interacts (via UBL domain) with
CC       MDN1 (via VWFA/MIDAS domain) (By similarity).
CC       {ECO:0000250|UniProtKB:P25382, ECO:0000250|UniProtKB:Q9NVX2}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NLE1/RSA4 family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; BT021777; AAX46624.1; -; mRNA.
DR   RefSeq; NP_001014887.1; NM_001014887.1.
DR   AlphaFoldDB; Q58D20; -.
DR   SMR; Q58D20; -.
DR   STRING; 9913.ENSBTAP00000025423; -.
DR   PaxDb; Q58D20; -.
DR   PRIDE; Q58D20; -.
DR   Ensembl; ENSBTAT00000025423; ENSBTAP00000025423; ENSBTAG00000019094.
DR   GeneID; 509793; -.
DR   KEGG; bta:509793; -.
DR   CTD; 54475; -.
DR   VEuPathDB; HostDB:ENSBTAG00000019094; -.
DR   VGNC; VGNC:32109; NLE1.
DR   eggNOG; KOG0271; Eukaryota.
DR   GeneTree; ENSGT00940000157881; -.
DR   HOGENOM; CLU_000288_57_16_1; -.
DR   InParanoid; Q58D20; -.
DR   OMA; HINCLAW; -.
DR   OrthoDB; 723347at2759; -.
DR   TreeFam; TF300668; -.
DR   Proteomes; UP000009136; Chromosome 19.
DR   Bgee; ENSBTAG00000019094; Expressed in retina and 106 other tissues.
DR   ExpressionAtlas; Q58D20; baseline and differential.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR   GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR024977; Apc4_WD40_dom.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR001632; Gprotein_B.
DR   InterPro; IPR012972; NLE.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   Pfam; PF12894; ANAPC4_WD40; 1.
DR   Pfam; PF08154; NLE; 1.
DR   Pfam; PF00400; WD40; 5.
DR   PRINTS; PR00319; GPROTEINB.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 8.
DR   SUPFAM; SSF50978; SSF50978; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 4.
DR   PROSITE; PS50082; WD_REPEATS_2; 7.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Notch signaling pathway; Nucleus; Phosphoprotein; Reference proteome;
KW   Repeat; WD repeat.
FT   CHAIN           1..486
FT                   /note="Notchless protein homolog 1"
FT                   /id="PRO_0000051097"
FT   REPEAT          113..152
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          155..194
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          198..242
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          245..283
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          326..367
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          371..410
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          413..452
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          455..486
FT                   /note="WD 8"
FT                   /evidence="ECO:0000255"
FT   REGION          15..97
FT                   /note="Ubiquitin-like (UBL) domain"
FT                   /evidence="ECO:0000250|UniProtKB:P25382"
FT   MOD_RES         80
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:Q9NVX2"
SQ   SEQUENCE   486 AA;  53229 MW;  B4C6A5B21FC24CDE CRC64;
     MAAAAAADEA ATRDVQRLLV QFQDEGGQLL GSPFDVPVDI TPDKLQLVCN ALLAQEDPLP
     LAFYVHDAEI VSSLGRTLES QAVETEKVLD IIYQPQAIFR VRAVTRCTSS LEGHSEAVIS
     VAFSPTGKYL ASGSGDTTVR FWDLSTETPH FTCQGHRHWV LSISWSPDGK KLASGCKNGQ
     ILLWDPSTGK QVGRALTGHS KWITALSWEP LHANPECRYV ASSSKDGSVR VWDTTAGRCE
     RTLTGHAQSV TCLRWGGDGL LYSASQDRTI KVWRAHDGVL CRTLQGHGHW VNTMALSTDY
     ALRTGAFEPA EASVNAQDLR GSLQELKERA LSRYNLVRGQ GPERLVSGSD DFTLFLWSPA
     EDKKPLARMT GHQALINQVV FSPDSRVIAS ASFDKSIKLW DGRTGKYLAS LRGHVAAVYQ
     IAWSADSRLL VSGSSDSTLK VWDVKAQKLS TDLPGHADEV YAVDWSPDGQ RVASGGKDKC
     LRIWRR