NLE1_SCHPO
ID NLE1_SCHPO Reviewed; 502 AA.
AC O74855;
DT 05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=Ribosome assembly protein 4 {ECO:0000250|UniProtKB:P25382};
DE AltName: Full=Notchless protein homolog 1 {ECO:0000250|UniProtKB:Q9VPR4};
DE AltName: Full=Ribosome biogenesis factor rsa4 {ECO:0000250|UniProtKB:Q9VPR4};
GN Name=rsa4; ORFNames=SPCC18.05c {ECO:0000312|PomBase:SPCC18.05c};
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Involved in ribosome biogenesis. Required for processing and
CC efficient intra-nuclear transport of pre-60S ribosomal subunits.
CC Interacts with the AAA-ATPase Midasin, which is essential for the ATP-
CC dependent dissociation of a group of nonribosomal factors from the pre-
CC 60S particle. {ECO:0000250|UniProtKB:P25382}.
CC -!- SUBUNIT: Associates with the pre-60S ribosomal particle. Interacts (via
CC WD repeats) with uL18 (rpl501/rpl502). Interacts (via UBL domain) with
CC mdn1 (via VWFA/MIDAS domain). Interacts (via WD repeats) with nsa2.
CC {ECO:0000250|UniProtKB:P25382}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the NLE1/RSA4 family. {ECO:0000305}.
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DR EMBL; CU329672; CAA21419.1; -; Genomic_DNA.
DR PIR; T41148; T41148.
DR RefSeq; NP_588384.1; NM_001023375.2.
DR AlphaFoldDB; O74855; -.
DR SMR; O74855; -.
DR BioGRID; 275946; 4.
DR STRING; 4896.SPCC18.05c.1; -.
DR iPTMnet; O74855; -.
DR MaxQB; O74855; -.
DR PaxDb; O74855; -.
DR EnsemblFungi; SPCC18.05c.1; SPCC18.05c.1:pep; SPCC18.05c.
DR GeneID; 2539380; -.
DR KEGG; spo:SPCC18.05c; -.
DR PomBase; SPCC18.05c; rsa4.
DR VEuPathDB; FungiDB:SPCC18.05c; -.
DR eggNOG; KOG0271; Eukaryota.
DR HOGENOM; CLU_000288_57_16_1; -.
DR InParanoid; O74855; -.
DR OMA; HINCLAW; -.
DR PhylomeDB; O74855; -.
DR Reactome; R-SPO-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:O74855; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005730; C:nucleolus; ISS:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0032991; C:protein-containing complex; NAS:PomBase.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR012972; NLE.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08154; NLE; 1.
DR Pfam; PF00400; WD40; 8.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 5.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Nucleus; Reference proteome; Repeat; Ribosome biogenesis; WD repeat.
FT CHAIN 1..502
FT /note="Ribosome assembly protein 4"
FT /id="PRO_0000316570"
FT REPEAT 132..172
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 175..214
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 218..264
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 267..307
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 344..383
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 387..426
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 429..468
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 471..502
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REGION 28..116
FT /note="Ubiquitin-like (UBL) domain"
FT /evidence="ECO:0000250|UniProtKB:P25382"
SQ SEQUENCE 502 AA; 56003 MW; 5773208C7DEEB23A CRC64;
MATLLPPKSK RQKKESLNPT TIEIPEKFPL VNVQFRASDD SNELASLLVP GNSSVRQLEA
LLNQLLENSD DPVPYNFALH HEDETIEIQD NLYTSVFHNG LMKTEDHLTL LYTPQAVFRV
RAVTRCTASM NGHDGTIISA QFSPSTSSRL VTGSGDFTAR LWDCDTQTPI ATMKGHTNWV
SCVAWAPDAS IIATGSMDNT IRFWDPKKGS PIGDALRRHT KPIMALCWQP LHLAPDSGPY
LLASGSKDNT VRIWNVKLRT LLFTLSGHTA PITCVRWGGQ NWIYSSSYDK TIRIWDAKDG
KCLHILKGHA ARVNHLSLST EHVLRSGAYD HTDFKPKSFS DERRKAKERY EACLKQSGER
LVSASDDLQL ILWDPQKSTK PITKMHGHQK VVNHASFSPD GRCIATASFD SSVRLWDGKT
GKFLATLRGH VAAVYQCAWS TDSRLLVSSS QDTTLKVWDV RSKKMKFDLP GHEDQVFAVD
WSPDGQRVAS GGADKAVRIW SH
