ID   NLE1_XENLA              Reviewed;         476 AA.
AC   Q7ZXK9; O93531;
DT   11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=Notchless protein homolog 1 {ECO:0000250|UniProtKB:Q9NVX2};
GN   Name=nle1 {ECO:0000312|Xenbase:XB-GENE-984064};
GN   Synonyms=nle {ECO:0000312|EMBL:AAC62236.1};
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAC62236.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=9857191; DOI=10.1093/emboj/17.24.7351;
RA   Royet J., Bouwmeester T., Cohen S.M.;
RT   "Notchless encodes a novel WD40-repeat-containing protein that modulates
RT   Notch signaling activity.";
RL   EMBO J. 17:7351-7360(1998).
RN   [2] {ECO:0000312|EMBL:AAH44710.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo {ECO:0000312|EMBL:AAH44710.1};
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in regulating the expression of CDKN1A and
CC       several members of the Wnt pathway, probably via its effects on Notch
CC       activity. Required for normal embryogenesis (By similarity). Plays a
CC       role in regulating Notch activity. {ECO:0000250,
CC       ECO:0000269|PubMed:9857191}.
CC   -!- SUBUNIT: Associates with the pre-60S ribosomal particle. Interacts (via
CC       WD repeats) with uL18 (By similarity). Interacts (via UBL domain) with
CC       MDN1 (via VWFA/MIDAS domain) (By similarity).
CC       {ECO:0000250|UniProtKB:P25382, ECO:0000250|UniProtKB:Q9NVX2}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q9NVX2}.
CC   -!- DEVELOPMENTAL STAGE: Maternally transcribed, with constant expression
CC       during early stages of embryogenesis. Levels increase at the end of
CC       gastrulation and are maintained during neurulation and organogenesis.
CC       During later stages expressed in somites, segmental plate, brain,
CC       branchial arches, eyes and ventral bood islands.
CC       {ECO:0000269|PubMed:9857191}.
CC   -!- SIMILARITY: Belongs to the NLE1/RSA4 family. {ECO:0000255}.
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DR   EMBL; AF069737; AAC62236.1; -; mRNA.
DR   EMBL; BC044710; AAH44710.1; -; mRNA.
DR   RefSeq; NP_001080675.1; NM_001087206.1.
DR   AlphaFoldDB; Q7ZXK9; -.
DR   SMR; Q7ZXK9; -.
DR   MaxQB; Q7ZXK9; -.
DR   PRIDE; Q7ZXK9; -.
DR   DNASU; 380367; -.
DR   GeneID; 380367; -.
DR   KEGG; xla:380367; -.
DR   CTD; 380367; -.
DR   Xenbase; XB-GENE-984064; nle1.S.
DR   OrthoDB; 723347at2759; -.
DR   Proteomes; UP000186698; Chromosome 2S.
DR   Bgee; 380367; Expressed in testis and 19 other tissues.
DR   GO; GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
DR   GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR   InterPro; IPR001632; Gprotein_B.
DR   InterPro; IPR012972; NLE.
DR   InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR019775; WD40_repeat_CS.
DR   Pfam; PF08154; NLE; 1.
DR   Pfam; PF00400; WD40; 7.
DR   PRINTS; PR00319; GPROTEINB.
DR   PRINTS; PR00320; GPROTEINBRPT.
DR   SMART; SM00320; WD40; 8.
DR   SUPFAM; SSF50998; SSF50998; 1.
DR   PROSITE; PS00678; WD_REPEATS_1; 4.
DR   PROSITE; PS50082; WD_REPEATS_2; 7.
DR   PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE   2: Evidence at transcript level;
KW   Notch signaling pathway; Nucleus; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..476
FT                   /note="Notchless protein homolog 1"
FT                   /id="PRO_0000424663"
FT   REPEAT          103..142
FT                   /note="WD 1"
FT                   /evidence="ECO:0000255"
FT   REPEAT          145..184
FT                   /note="WD 2"
FT                   /evidence="ECO:0000255"
FT   REPEAT          189..234
FT                   /note="WD 3"
FT                   /evidence="ECO:0000255"
FT   REPEAT          236..273
FT                   /note="WD 4"
FT                   /evidence="ECO:0000255"
FT   REPEAT          311..357
FT                   /note="WD 5"
FT                   /evidence="ECO:0000255"
FT   REPEAT          361..400
FT                   /note="WD 6"
FT                   /evidence="ECO:0000255"
FT   REPEAT          403..442
FT                   /note="WD 7"
FT                   /evidence="ECO:0000255"
FT   REPEAT          445..476
FT                   /note="WD 8"
FT                   /evidence="ECO:0000255"
FT   REGION          5..87
FT                   /note="Ubiquitin-like (UBL) domain"
FT                   /evidence="ECO:0000250|UniProtKB:P25382"
FT   CONFLICT        28
FT                   /note="V -> L (in Ref. 1; AAC62236)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   476 AA;  52851 MW;  1EE7BE6806FF5CC1 CRC64;
     MKEDVGRLLI QFKNENGEGL GTPFDVPVDI TPDKLQLVCN ALLQEEDPVP LAFFVQDLEI
     VTSLDKTLEK QSVETEKVID IIYQPQAVFK VRAVTRCTSS LEGHTEAVIS VAFSPTGKYL
     ASGSGDTTVR FWDLSTETPH FTSKGHTHWV LSIAWSPDGK KLASGCKNSQ IFIWDPSTGK
     QIGKPLTGHS KWITWLCWEP LHLNPESRYL ASASKDCTIR IWDTVMGQCQ KILTSHTQSV
     TAVKWGGDGL LYSSSQDRTI KAWRAQDGVL CRTLQGHAHW VNTMALSTDY VLRTGAFNPA
     DASVNPQDMS GSLEVLKEKA LKRYNEVRGQ GPERLVSGSD DFTLFLWAPA EEKKPLQRMT
     GHQALINEVL FSPDTRIIAS ASFDKSIKLW DGKTGKFLTS LRGHVSAVYQ IAWSADSRLL
     VSGSSDSTLK VWDSKTKKLL IDLPGHADEV YSVDWSPDGQ RVASGGKDKC LRIWRK