NLE_DROME
ID NLE_DROME Reviewed; 488 AA.
AC Q9VPR4; O96995; Q8T4A2;
DT 11-DEC-2013, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 2.
DT 03-AUG-2022, entry version 168.
DE RecName: Full=Protein Notchless {ECO:0000303|PubMed:9857191};
GN Name=Nle {ECO:0000312|EMBL:AAF51479.2, ECO:0000312|FlyBase:FBgn0021874};
GN ORFNames=CG2863;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF51479.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|EMBL:AAF51479.2}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAL90024.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Testis {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305, ECO:0000312|EMBL:CAA10070.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 8-488, FUNCTION, AND INTERACTION WITH NOTCH.
RX PubMed=9857191; DOI=10.1093/emboj/17.24.7351;
RA Royet J., Bouwmeester T., Cohen S.M.;
RT "Notchless encodes a novel WD40-repeat-containing protein that modulates
RT Notch signaling activity.";
RL EMBO J. 17:7351-7360(1998).
CC -!- FUNCTION: Plays a role in regulating Notch activity.
CC {ECO:0000269|PubMed:9857191}.
CC -!- SUBUNIT: Interacts with Notch (via cytoplasmic domain)
CC (PubMed:9857191). Associates with the pre-60S ribosomal particle (By
CC similarity). {ECO:0000250|UniProtKB:P25382,
CC ECO:0000269|PubMed:9857191}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- MISCELLANEOUS: The name 'Notchless' derives from the ability to
CC suppress the wing notching caused by some Notch mutant alleles.
CC {ECO:0000303|PubMed:9857191}.
CC -!- SIMILARITY: Belongs to the NLE1/RSA4 family. {ECO:0000255}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014134; AAF51479.2; -; Genomic_DNA.
DR EMBL; AY089286; AAL90024.1; -; mRNA.
DR EMBL; AJ012588; CAA10070.1; -; mRNA.
DR RefSeq; NP_477294.2; NM_057946.4.
DR AlphaFoldDB; Q9VPR4; -.
DR SMR; Q9VPR4; -.
DR BioGRID; 59490; 5.
DR IntAct; Q9VPR4; 2.
DR STRING; 7227.FBpp0077735; -.
DR PaxDb; Q9VPR4; -.
DR PRIDE; Q9VPR4; -.
DR DNASU; 33234; -.
DR EnsemblMetazoa; FBtr0078075; FBpp0077735; FBgn0021874.
DR GeneID; 33234; -.
DR KEGG; dme:Dmel_CG2863; -.
DR UCSC; CG2863-RA; d. melanogaster.
DR CTD; 33234; -.
DR FlyBase; FBgn0021874; Nle.
DR VEuPathDB; VectorBase:FBgn0021874; -.
DR eggNOG; KOG0271; Eukaryota.
DR GeneTree; ENSGT00940000157881; -.
DR HOGENOM; CLU_000288_57_16_1; -.
DR InParanoid; Q9VPR4; -.
DR OMA; HINCLAW; -.
DR OrthoDB; 723347at2759; -.
DR PhylomeDB; Q9VPR4; -.
DR Reactome; R-DME-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q9VPR4; -.
DR BioGRID-ORCS; 33234; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 33234; -.
DR PRO; PR:Q9VPR4; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0021874; Expressed in eye disc (Drosophila) and 43 other tissues.
DR Genevisible; Q9VPR4; DM.
DR GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR GO; GO:0005112; F:Notch binding; IDA:FlyBase.
DR GO; GO:0045746; P:negative regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0007219; P:Notch signaling pathway; IBA:GO_Central.
DR GO; GO:0045747; P:positive regulation of Notch signaling pathway; IMP:FlyBase.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR012972; NLE.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF08154; NLE; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 7.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Notch signaling pathway; Nucleus; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..488
FT /note="Protein Notchless"
FT /id="PRO_0000424662"
FT REPEAT 117..156
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 159..198
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 202..246
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 249..287
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 329..370
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 373..412
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 415..454
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 457..488
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 19..101
FT /note="Ubiquitin-like (UBL) domain"
FT /evidence="ECO:0000250|UniProtKB:P25382"
FT CONFLICT 28..29
FT /note="SD -> Y (in Ref. 4; CAA10070)"
FT /evidence="ECO:0000305"
FT CONFLICT 291
FT /note="H -> Q (in Ref. 4; CAA10070)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 488 AA; 53810 MW; D4883202954A7785 CRC64;
MLAKKQKMQE TDTEQEATPH TIQARLVSDT GEEAGPPIDL PAGITTQQLG LICNALLKNE
EATPYLFFVG EDEIKKSLED TLDLASVDTE NVIDIVYQPQ AVFKVRPVTR CTSSMPGHAE
AVVSLNFSPD GAHLASGSGD TTVRLWDLNT ETPHFTCTGH KQWVLCVSWA PDGKRLASGC
KAGSIIIWDP ETGQQKGRPL SGHKKHINCL AWEPYHRDPE CRKLASASGD GDCRIWDVKL
GQCLMNIAGH TNAVTAVRWG GAGLIYTSSK DRTVKMWRAA DGILCRTFSG HAHWVNNIAL
STDYVLRTGP FHPVKDRSKS HLSLSTEELQ ESALKRYQAV CPDEVESLVS CSDDNTLYLW
RNNQNKCVER MTGHQNVVND VKYSPDVKLI ASASFDKSVR LWRASDGQYM ATFRGHVQAV
YTVAWSADSR LIVSGSKDST LKVWSVQTKK LAQELPGHAD EVFGVDWAPD GSRVASGGKD
KVIKLWAY
