ID   NLHAP_HALAA             Reviewed;         352 AA.
AC   A0A1W5X0D5;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2017, sequence version 1.
DT   25-MAY-2022, entry version 8.
DE   RecName: Full=Sodium-lithium/proton antiporter {ECO:0000305};
DE   AltName: Full=Na(+) (Li(+))/H(+) antiporter {ECO:0000303|PubMed:28374790};
OS   Halobacillus andaensis.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Halobacillus.
OX   NCBI_TaxID=1176239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES,
RP   AND SUBCELLULAR LOCATION.
RC   STRAIN=DSM 25866 / CGMCC 1.12153 / NEAU-ST10-40;
RX   PubMed=28374790; DOI=10.1038/srep45936;
RA   Dong P., Wang L., Song N., Yang L., Chen J., Yan M., Chen H., Zhang R.,
RA   Li J., Abdel-Motaal H., Jiang J.;
RT   "A UPF0118 family protein with uncharacterized function from the moderate
RT   halophile Halobacillus andaensis represents a novel class of Na+(Li+)/H+
RT   antiporter.";
RL   Sci. Rep. 7:45936-45936(2017).
CC   -!- FUNCTION: Catalyzes the pH-dependent efflux of sodium and lithium in
CC       exchange for external protons. {ECO:0000269|PubMed:28374790}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=1.13 mM for Na(+) {ECO:0000269|PubMed:28374790};
CC         KM=1.50 mM for Li(+) {ECO:0000269|PubMed:28374790};
CC       pH dependence:
CC         Optimum pH is 9.0. {ECO:0000269|PubMed:28374790};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28374790};
CC       Multi-pass membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86)
CC       family. {ECO:0000305}.
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DR   EMBL; KY231907; ARH13824.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1W5X0D5; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015297; F:antiporter activity; IEA:UniProtKB-KW.
DR   GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR   InterPro; IPR002549; AI-2E-like.
DR   InterPro; IPR014227; YtvI-like.
DR   PANTHER; PTHR21716; PTHR21716; 1.
DR   Pfam; PF01594; AI-2E_transport; 1.
DR   TIGRFAMs; TIGR02872; spore_ytvI; 1.
PE   1: Evidence at protein level;
KW   Antiport; Cell membrane; Ion transport; Membrane; Sodium; Sodium transport;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..352
FT                   /note="Sodium-lithium/proton antiporter"
FT                   /id="PRO_0000441404"
FT   TRANSMEM        11..31
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        32..52
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        61..81
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        216..236
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        241..261
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        271..291
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   352 AA;  39629 MW;  3483BF10F6E3E3A3 CRC64;
     MFRYLSKRQW ILLLLGILFV VAGYFILPVS VPLIIALITA LFLNPAVRWM QFRFRLNRKM
     AVTIVFLLFV IMIGLLGTYA VTRAVTQLVE LADNAPSYIN QINNVLINWQ NNMNSFTQNM
     PSEFVDKVSV ELQNTIDTTT QTLSQKLQLS NIAAFAAKIP EYLISFLVYL IALFLFMLEL
     PRLKDKMHGN FTESTSEKVK FMNARLSYVV FGFLKAQFLV SIVIFVVCLI GLFWITPEVA
     IVMSLIIWIV DFVPIIGSIV ILGPWALYML IVGDIAMGGQ LAMLAIILLA IRRTVEPKVM
     GRHIGLSPLA TLIAMYIGLQ LIGLMGFILG PLLVIAFNSA KEAGIIRWNF KL