ID   NLP1_PHYCP              Reviewed;         234 AA.
AC   L7NCR0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   03-AUG-2022, entry version 16.
DE   RecName: Full=NLP effector protein 1 {ECO:0000303|PubMed:24886309};
DE   AltName: Full=Necrosis-inducing protein 1 {ECO:0000303|PubMed:21644208};
DE   AltName: Full=Nep1-like protein 1 {ECO:0000303|PubMed:21644208};
DE   Flags: Precursor;
GN   Name=NLP1 {ECO:0000303|PubMed:24886309};
GN   Synonyms=NPP1 {ECO:0000303|PubMed:21644208};
OS   Phytophthora capsici.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4784;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DOMAIN.
RX   PubMed=21644208; DOI=10.4238/vol10-2gmr1248;
RA   Feng B.Z., Li P.Q., Fu L., Sun B.B., Zhang X.G.;
RT   "Identification of 18 genes encoding necrosis-inducing proteins from the
RT   plant pathogen Phytophthora capsici (Pythiaceae: Oomycetes).";
RL   Genet. Mol. Res. 10:910-922(2011).
RN   [2]
RP   INDUCTION, AND FUNCTION.
RX   PubMed=24886309; DOI=10.1186/1471-2229-14-126;
RA   Feng B.Z., Zhu X.P., Fu L., Lv R.F., Storey D., Tooley P., Zhang X.G.;
RT   "Characterization of necrosis-inducing NLP proteins in Phytophthora
RT   capsici.";
RL   BMC Plant Biol. 14:126-126(2014).
CC   -!- FUNCTION: Secreted effector that contributes to virulence during
CC       infection by P.capsici. Induces distinct chlorosis at 3 days after
CC       inoculation of host C.annuum leaves, and all the chlorotic areas
CC       gradually turn brown and become moderately necrotic at 7 days after
CC       inoculation. Leads only to chlorotic areas, without necrosis at 7 days
CC       after non-host N.benthamiana leaves infection.
CC       {ECO:0000269|PubMed:24886309}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:21644208}.
CC   -!- INDUCTION: Expression reached the highest levels at 3 days after
CC       inoculation of pepper leaves, followed by a gradual decline.
CC       {ECO:0000269|PubMed:24886309}.
CC   -!- DOMAIN: The structure of NLP effectors is remarkably conserved with a
CC       high level of conservation of a central hepta-peptide motif GHRHDWE.
CC       {ECO:0000305|PubMed:21644208}.
CC   -!- SIMILARITY: Belongs to the Necrosis inducing protein (NPP1) family.
CC       {ECO:0000305}.
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DR   EMBL; HM543167; AEJ88232.1; -; Genomic_DNA.
DR   AlphaFoldDB; L7NCR0; -.
DR   SMR; L7NCR0; -.
DR   VEuPathDB; FungiDB:DVH05_028340; -.
DR   PHI-base; PHI:8061; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008701; NPP1.
DR   PANTHER; PTHR33657; PTHR33657; 1.
DR   Pfam; PF05630; NPP1; 1.
DR   PIRSF; PIRSF029958; Necrosis-inducing_protein; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Secreted; Signal; Virulence.
FT   SIGNAL          1..18
FT                   /evidence="ECO:0000255"
FT   CHAIN           19..234
FT                   /note="NLP effector protein 1"
FT                   /id="PRO_5003982453"
FT   MOTIF           119..125
FT                   /note="Hepta-peptide GHRHDWE motif"
FT                   /evidence="ECO:0000305|PubMed:24886309"
FT   CARBOHYD        66
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   234 AA;  25647 MW;  AAC6771EB6030BD1 CRC64;
     MQLRAFISVF ASLACVNAAV IDHDQVVPFA QPTPTTTLQT LAVQYKPQIY IANGCHPYPA
     VDEDGNTSGG LKPTGSQSAG CKGSGYGSQI YGRAVAYEGV YAFMYSWYMP KDETLPGLGH
     RHDWEACVVW LDSLENPNVV ALSASYHSTY LTYYPPDSDY LDGNSAKIEY STSWVILDHL
     LSATSTSGET QDLIMWDQLT DAARTALEDT DFGDANVPFK DANFETKLAN AYYT