ID   NLP8_CAEEL              Reviewed;         172 AA.
AC   Q93409;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Neuropeptide-like protein nlp-8 {ECO:0000312|WormBase:D2005.2};
DE   Contains:
DE     RecName: Full=AFDRMDNSDFFGA {ECO:0000305|PubMed:30229400};
DE   Contains:
DE     RecName: Full=SFDRMGGTEFGLM {ECO:0000305|PubMed:30229400};
DE   Flags: Precursor;
GN   Name=nlp-8 {ECO:0000312|WormBase:D2005.2};
GN   ORFNames=D2005.2 {ECO:0000312|WormBase:D2005.2};
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239 {ECO:0000312|Proteomes:UP000001940};
RN   [1] {ECO:0000312|Proteomes:UP000001940}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [2] {ECO:0000305}
RP   FUNCTION, AND DEVELOPMENTAL STAGE.
RX   PubMed=27546573; DOI=10.1016/j.cub.2016.07.048;
RA   Nath R.D., Chow E.S., Wang H., Schwarz E.M., Sternberg P.W.;
RT   "C. elegans Stress-Induced Sleep Emerges from the Collective Action of
RT   Multiple Neuropeptides.";
RL   Curr. Biol. 26:2446-2455(2016).
RN   [3] {ECO:0000305}
RP   FUNCTION, PROTEOLYTIC CLEAVAGE, AND MASS SPECTROMETRY.
RX   PubMed=30229400; DOI=10.1007/s11064-018-2636-2;
RA   Salem J.B., Nkambeu B., Arvanitis D.N., Beaudry F.;
RT   "Deciphering the Role of EGL-3 for Neuropeptides Processing in
RT   Caenorhabditis elegans Using High-Resolution Quadrupole-Orbitrap Mass
RT   Spectrometry.";
RL   Neurochem. Res. 43:2121-2131(2018).
CC   -!- FUNCTION: Neuropeptide-like protein (PubMed:30229400). Plays a role in
CC       behaviors associated with a sleep-like state induced by stress (SIS),
CC       acting in concert with the FARP (FMRFamide related) peptides, flp-13
CC       and flp-24 (PubMed:27546573). {ECO:0000269|PubMed:27546573,
CC       ECO:0000269|PubMed:30229400}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the ALA neuron in L4 stage larvae.
CC       {ECO:0000269|PubMed:27546573}.
CC   -!- PTM: May be processed by convertase egl-3.
CC       {ECO:0000269|PubMed:30229400}.
CC   -!- MASS SPECTROMETRY: [AFDRMDNSDFFGA]: Mass=746.8; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:30229400};
CC   -!- MASS SPECTROMETRY: [SFDRMGGTEFGLM]: Mass=724.3; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:30229400};
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DR   EMBL; BX284601; CAB02079.1; -; Genomic_DNA.
DR   PIR; T20329; T20329.
DR   RefSeq; NP_492158.1; NM_059757.5.
DR   AlphaFoldDB; Q93409; -.
DR   STRING; 6239.D2005.2; -.
DR   EPD; Q93409; -.
DR   PaxDb; Q93409; -.
DR   EnsemblMetazoa; D2005.2.1; D2005.2.1; WBGene00003746.
DR   GeneID; 172544; -.
DR   KEGG; cel:CELE_D2005.2; -.
DR   UCSC; D2005.2; c. elegans.
DR   CTD; 172544; -.
DR   WormBase; D2005.2; CE09066; WBGene00003746; nlp-8.
DR   eggNOG; ENOG502TGUI; Eukaryota.
DR   HOGENOM; CLU_1571991_0_0_1; -.
DR   InParanoid; Q93409; -.
DR   OMA; YPYLIFP; -.
DR   OrthoDB; 1458068at2759; -.
DR   Proteomes; UP000001940; Chromosome I.
DR   Bgee; WBGene00003746; Expressed in larva and 3 other tissues.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0007218; P:neuropeptide signaling pathway; IEA:UniProtKB-KW.
DR   GO; GO:0030431; P:sleep; IGI:UniProtKB.
PE   1: Evidence at protein level;
KW   Neuropeptide; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..26
FT                   /evidence="ECO:0000255"
FT   CHAIN           27..172
FT                   /note="Neuropeptide-like protein nlp-8"
FT                   /id="PRO_0000454192"
FT   PEPTIDE         100..112
FT                   /note="AFDRMDNSDFFGA"
FT                   /evidence="ECO:0000269|PubMed:30229400"
FT                   /id="PRO_0000454193"
FT   PEPTIDE         117..129
FT                   /note="SFDRMGGTEFGLM"
FT                   /evidence="ECO:0000269|PubMed:30229400"
FT                   /id="PRO_0000454194"
SQ   SEQUENCE   172 AA;  19775 MW;  7C72E19EAE4BC251 CRC64;
     MSQKLLPISP LQLLFLQCLL IGFTAAYPYL IFPASPSSGD SRRLVKRAFD RFDNSGVFSF
     GAKRFDRYDD ETAYGYGFDN HIFKRSADPY RFMSVPTKKA FDRMDNSDFF GAKRKRSFDR
     MGGTEFGLMK RSAPESREQL INNLAESIIT LRRAREAESS PESQRTIITY DD