ID   NLP8_PHYCP              Reviewed;         316 AA.
AC   L7NCQ0;
DT   05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT   03-APR-2013, sequence version 1.
DT   03-AUG-2022, entry version 17.
DE   RecName: Full=NLP effector protein 8 {ECO:0000303|PubMed:24886309};
DE   AltName: Full=Necrosis-inducing protein 8 {ECO:0000303|PubMed:21644208};
DE   AltName: Full=Nep1-like protein 8 {ECO:0000303|PubMed:21644208};
DE   Flags: Precursor;
GN   Name=NLP8 {ECO:0000303|PubMed:24886309};
GN   Synonyms=NPP8 {ECO:0000303|PubMed:21644208};
OS   Phytophthora capsici.
OC   Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC   Phytophthora.
OX   NCBI_TaxID=4784;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DOMAIN.
RX   PubMed=21644208; DOI=10.4238/vol10-2gmr1248;
RA   Feng B.Z., Li P.Q., Fu L., Sun B.B., Zhang X.G.;
RT   "Identification of 18 genes encoding necrosis-inducing proteins from the
RT   plant pathogen Phytophthora capsici (Pythiaceae: Oomycetes).";
RL   Genet. Mol. Res. 10:910-922(2011).
RN   [2]
RP   INDUCTION, AND FUNCTION.
RX   PubMed=24886309; DOI=10.1186/1471-2229-14-126;
RA   Feng B.Z., Zhu X.P., Fu L., Lv R.F., Storey D., Tooley P., Zhang X.G.;
RT   "Characterization of necrosis-inducing NLP proteins in Phytophthora
RT   capsici.";
RL   BMC Plant Biol. 14:126-126(2014).
CC   -!- FUNCTION: Secreted effector that contributes moderately to virulence
CC       during infection by P.capsici. Does not cause visible reaction of
CC       C.annuum for several days after inoculation, but by 7 days after
CC       inoculation, small necrotic lesions become visible. Leads only to
CC       chlorotic areas, without necrosis at 7 days after non-host
CC       N.benthamiana leaves infection. {ECO:0000269|PubMed:24886309}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:21644208}.
CC   -!- INDUCTION: Expression is induced during infection of host leaves, but
CC       stays low compared to other NLP effectors.
CC       {ECO:0000269|PubMed:24886309}.
CC   -!- DOMAIN: The structure of NLP effectors is remarkably conserved with a
CC       high level of conservation of a central hepta-peptide motif GHRHDWE.
CC       {ECO:0000305|PubMed:21644208}.
CC   -!- SIMILARITY: Belongs to the Necrosis inducing protein (NPP1) family.
CC       {ECO:0000305}.
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DR   EMBL; HM543174; AEJ88239.1; -; Genomic_DNA.
DR   AlphaFoldDB; L7NCQ0; -.
DR   SMR; L7NCQ0; -.
DR   VEuPathDB; FungiDB:DVH05_022934; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008701; NPP1.
DR   PANTHER; PTHR33657; PTHR33657; 1.
DR   Pfam; PF05630; NPP1; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Secreted; Signal; Virulence.
FT   SIGNAL          1..19
FT                   /evidence="ECO:0000255"
FT   CHAIN           20..316
FT                   /note="NLP effector protein 8"
FT                   /id="PRO_5003982509"
FT   REGION          62..100
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           199..205
FT                   /note="Hepta-peptide GHRHDWE motif"
FT                   /evidence="ECO:0000305|PubMed:24886309"
FT   COMPBIAS        70..94
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        24
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   316 AA;  34437 MW;  D0715B2CBEDACB49 CRC64;
     MRLSIALGAV LTVLIAVQGE ETTNSTTKYI DYSSLKKFTL APTIQQSSTD GSQVQQIQIP
     ISTRPDPRAT EAPTPAPTLA PTPAPTPTPT PDPGPWTTIT IDHDQVKPFA QPEPVTISEK
     TAVKFKPQLH ITNGCHSYPA VDEAGETGGG LKTKGAPSAG CKGSGWGSQV YGRSGWYQDV
     WAIMYCWYFP KDEPSSGIGH RHDWEHVIVW IDNPAVENPK ILAVTPSAHS GYHKYAPPNA
     DTIDGVTTKV AYDSKWPMDH ALDSTSEAGD FQDLIMWDQL SENARRALNS VNFGRPNTPM
     NDGNFQPKLA KAWPFQ