ID   NLPA_ECOLI              Reviewed;         272 AA.
AC   P04846; Q2M7X3;
DT   13-AUG-1987, integrated into UniProtKB/Swiss-Prot.
DT   13-AUG-1987, sequence version 1.
DT   03-AUG-2022, entry version 158.
DE   RecName: Full=Lipoprotein 28;
DE   Flags: Precursor;
GN   Name=nlpA; OrderedLocusNames=b3661, JW3635;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DIACYLGLYCEROL AT CYS-24.
RX   PubMed=3003106; DOI=10.1016/s0021-9258(17)35931-8;
RA   Yu F., Inouye S., Inouye M.;
RT   "Lipoprotein-28, a cytoplasmic membrane lipoprotein from Escherichia coli.
RT   Cloning, DNA sequence, and expression of its gene.";
RL   J. Biol. Chem. 261:2284-2288(1986).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=7686882; DOI=10.1006/geno.1993.1230;
RA   Burland V.D., Plunkett G. III, Daniels D.L., Blattner F.R.;
RT   "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome:
RT   organizational symmetry around the origin of replication.";
RL   Genomics 16:551-561(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Lipid-anchor.
CC   -!- SIMILARITY: Belongs to the NlpA lipoprotein family. {ECO:0000305}.
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DR   EMBL; M12163; AAA24080.1; -; Genomic_DNA.
DR   EMBL; L10328; AAA62013.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76684.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77633.1; -; Genomic_DNA.
DR   PIR; A26286; LPEC28.
DR   RefSeq; NP_418117.1; NC_000913.3.
DR   RefSeq; WP_000779426.1; NZ_SSZK01000043.1.
DR   AlphaFoldDB; P04846; -.
DR   SMR; P04846; -.
DR   BioGRID; 4259626; 12.
DR   BioGRID; 852481; 1.
DR   IntAct; P04846; 8.
DR   STRING; 511145.b3661; -.
DR   SWISS-2DPAGE; P04846; -.
DR   jPOST; P04846; -.
DR   PaxDb; P04846; -.
DR   PRIDE; P04846; -.
DR   EnsemblBacteria; AAC76684; AAC76684; b3661.
DR   EnsemblBacteria; BAE77633; BAE77633; BAE77633.
DR   GeneID; 66672443; -.
DR   GeneID; 948175; -.
DR   KEGG; ecj:JW3635; -.
DR   KEGG; eco:b3661; -.
DR   PATRIC; fig|1411691.4.peg.3045; -.
DR   EchoBASE; EB0651; -.
DR   eggNOG; COG1464; Bacteria.
DR   HOGENOM; CLU_067080_0_0_6; -.
DR   InParanoid; P04846; -.
DR   OMA; QDNKAHN; -.
DR   PhylomeDB; P04846; -.
DR   BioCyc; EcoCyc:EG10657-MON; -.
DR   PRO; PR:P04846; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   InterPro; IPR004872; Lipoprotein_NlpA.
DR   PANTHER; PTHR30429; PTHR30429; 1.
DR   Pfam; PF03180; Lipoprotein_9; 1.
DR   PIRSF; PIRSF002854; MetQ; 1.
DR   TIGRFAMs; TIGR00363; TIGR00363; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW   Reference proteome; Signal.
FT   SIGNAL          1..23
FT   CHAIN           24..272
FT                   /note="Lipoprotein 28"
FT                   /id="PRO_0000019737"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000305"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303,
FT                   ECO:0000269|PubMed:3003106"
SQ   SEQUENCE   272 AA;  29424 MW;  E3C249E753AB1B33 CRC64;
     MKLTTHHLRT GAALLLAGIL LAGCDQSSSD AKHIKVGVIN GAEQDVAEVA KKVAKEKYGL
     DVELVGFSGS LLPNDATNHG ELDANVFQHR PFLEQDNQAH GYKLVAVGNT FVFPMAGYSK
     KIKTVAQIKE GATVAIPNDP TNLGRALLLL QKEKLITLKE GKGLLPTALD ITDNPRHLQI
     MELEGAQLPR VLDDPKVDVA IISTTYIQQT GLSPVHDSVF IEDKNSPYVN ILVAREDNKN
     AENVKEFLQS YQSPEVAKAA ETIFNGGAVP GW