NLPC_ECOLI
ID NLPC_ECOLI Reviewed; 154 AA.
AC P23898;
DT 01-NOV-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1991, sequence version 1.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Probable endopeptidase NlpC;
DE EC=3.4.-.-;
DE AltName: Full=ORF-17;
DE AltName: Full=Probable lipoprotein NlpC;
DE Flags: Precursor;
GN Name=nlpC; OrderedLocusNames=b1708, JW1698;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Kadner R.J.;
RL Submitted (FEB-1989) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097039; DOI=10.1093/dnares/3.6.363;
RA Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K.,
RA Miki T., Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S.,
RA Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G.,
RA Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y.,
RA Wada C., Yamamoto Y., Horiuchi T.;
RT "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 28.0-40.1 min region on the linkage map.";
RL DNA Res. 3:363-377(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the peptidase C40 family. {ECO:0000255|PROSITE-
CC ProRule:PRU01284, ECO:0000305}.
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DR EMBL; M14031; AAA23529.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74778.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15476.1; -; Genomic_DNA.
DR PIR; D64929; D64929.
DR RefSeq; NP_416223.1; NC_000913.3.
DR RefSeq; WP_001209795.1; NZ_STEB01000009.1.
DR AlphaFoldDB; P23898; -.
DR SMR; P23898; -.
DR BioGRID; 4260300; 304.
DR IntAct; P23898; 1.
DR STRING; 511145.b1708; -.
DR MEROPS; C40.A01; -.
DR PaxDb; P23898; -.
DR PRIDE; P23898; -.
DR EnsemblBacteria; AAC74778; AAC74778; b1708.
DR EnsemblBacteria; BAA15476; BAA15476; BAA15476.
DR GeneID; 946220; -.
DR KEGG; ecj:JW1698; -.
DR KEGG; eco:b1708; -.
DR PATRIC; fig|1411691.4.peg.549; -.
DR EchoBASE; EB1123; -.
DR eggNOG; COG0791; Bacteria.
DR HOGENOM; CLU_016043_9_3_6; -.
DR InParanoid; P23898; -.
DR OMA; WKRAYWQ; -.
DR PhylomeDB; P23898; -.
DR BioCyc; EcoCyc:EG11133-MON; -.
DR PRO; PR:P23898; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR InterPro; IPR000064; NLP_P60_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR Pfam; PF00877; NLPC_P60; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS51935; NLPC_P60; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane; Hydrolase; Lipoprotein; Membrane; Palmitate; Protease;
KW Reference proteome; Signal; Thiol protease.
FT SIGNAL 1..15
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 16..154
FT /note="Probable endopeptidase NlpC"
FT /id="PRO_0000019754"
FT DOMAIN 32..154
FT /note="NlpC/P60"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 62
FT /note="Nucleophile"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 115
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT ACT_SITE 127
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01284"
FT LIPID 16
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 16
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 154 AA; 17283 MW; CCAD869AF624BC4D CRC64;
MRFCLILITA LLLAGCSHHK APPPNARLSD SITVIAGLND QLQSWHGTPY RYGGMTRRGV
DCSGFVVVTM RDRFDLQLPR ETKQQASIGT QIDKDELLPG DLVFFKTGSG QNGLHVGIYD
TNNQFIHAST SKGVMRSSLD NVYWQKNFWQ ARRI
