NLPI_ECOKI
ID NLPI_ECOKI Reviewed; 294 AA.
AC D5D1H3;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 15-JUN-2010, sequence version 1.
DT 25-MAY-2022, entry version 56.
DE RecName: Full=Lipoprotein NlpI;
DE Flags: Precursor;
GN Name=nlpI; OrderedLocusNames=ECOK1_3584;
OS Escherichia coli O18:K1:H7 (strain IHE3034 / ExPEC).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=714962;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=IHE3034 / ExPEC;
RX PubMed=20439758; DOI=10.1073/pnas.0915077107;
RA Moriel D.G., Bertoldi I., Spagnuolo A., Marchi S., Rosini R., Nesta B.,
RA Pastorello I., Corea V.A., Torricelli G., Cartocci E., Savino S.,
RA Scarselli M., Dobrindt U., Hacker J., Tettelin H., Tallon L.J.,
RA Sullivan S., Wieler L.H., Ewers C., Pickard D., Dougan G., Fontana M.R.,
RA Rappuoli R., Pizza M., Serino L.;
RT "Identification of protective and broadly conserved vaccine antigens from
RT the genome of extraintestinal pathogenic Escherichia coli.";
RL Proc. Natl. Acad. Sci. U.S.A. 107:9072-9077(2010).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=O18:K1:H7 / RS218;
RX PubMed=20421385; DOI=10.1128/iai.00034-10;
RA Teng C.H., Tseng Y.T., Maruvada R., Pearce D., Xie Y., Paul-Satyaseela M.,
RA Kim K.S.;
RT "NlpI contributes to Escherichia coli K1 strain RS218 interaction with
RT human brain microvascular endothelial cells.";
RL Infect. Immun. 78:3090-3096(2010).
CC -!- FUNCTION: May be involved in cell division. May play a role in
CC bacterial septation or regulation of cell wall degradation during cell
CC division (By similarity). Maybe also be involved in pathogenicity by
CC playing a role in binding to and invading human brain microvascular
CC endothelial cells via cytosolic phospholipase A2 alpha activation.
CC {ECO:0000250, ECO:0000269|PubMed:20421385}.
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|PROSITE-
CC ProRule:PRU00303, ECO:0000269|PubMed:20421385}; Lipid-anchor
CC {ECO:0000255|PROSITE-ProRule:PRU00303, ECO:0000269|PubMed:20421385}.
CC -!- DISRUPTION PHENOTYPE: Reduced binding to and invasion of HBMECs. Growth
CC defect in low-salt medium at 42 degree Celsius.
CC {ECO:0000269|PubMed:20421385}.
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DR EMBL; CP001969; ADE91500.1; -; Genomic_DNA.
DR RefSeq; WP_000802080.1; NC_017628.1.
DR AlphaFoldDB; D5D1H3; -.
DR SMR; D5D1H3; -.
DR GeneID; 67414899; -.
DR KEGG; eih:ECOK1_3584; -.
DR PATRIC; fig|714962.3.peg.3621; -.
DR HOGENOM; CLU_071600_0_0_6; -.
DR OMA; VEHRYSF; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR023605; Lipoprotein_NlpI.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR013105; TPR_2.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF07719; TPR_2; 1.
DR Pfam; PF13181; TPR_8; 1.
DR PIRSF; PIRSF004654; NlpI; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
DR PROSITE; PS50005; TPR; 3.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 3: Inferred from homology;
KW Cell cycle; Cell division; Cell membrane; Lipoprotein; Membrane; Palmitate;
KW Repeat; Signal; TPR repeat.
FT SIGNAL 1..18
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 19..294
FT /note="Lipoprotein NlpI"
FT /id="PRO_0000413476"
FT REPEAT 62..95
FT /note="TPR 1"
FT REPEAT 96..129
FT /note="TPR 2"
FT REPEAT 234..267
FT /note="TPR 3"
FT LIPID 19
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT LIPID 19
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ SEQUENCE 294 AA; 33621 MW; 4CA6724327A9CEE7 CRC64;
MKPFLRWCFV ATALTLAGCS NTSWRKSEVL AVPLQPTLQQ EVILARMEQI LASRALTDDE
RAQLLYERGV LYDSLGLRAL ARNDFSQALA IRPDMPEVFN YLGIYLTQAG NFDAAYEAFD
SVLELDPTYN YAHLNRGIAL YYGGRDKLAQ DDLLAFYQDD PNDPFRSLWL YLAEQKLDEK
QAKEVLKQHF EKSDKEQWGW NIVEFYLGNI SEQTLMERLK ADATDNTSLA EHLSETNFYL
GKYYLSLGDL DSATALFKLA VANNVHNFVE HRYALLELSL LGQDQDDLAE SDQQ
