ID   NLRC4_XENTR             Reviewed;         997 AA.
AC   F6R2G2; Q28DS5;
DT   31-OCT-2012, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 2.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=NLR family CARD domain-containing protein 4;
DE   AltName: Full=Ice protease-activating factor;
DE            Short=Ipaf;
GN   Name=nlrc4; Synonyms=ipaf; ORFNames=TGas028l14.1;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=20431018; DOI=10.1126/science.1183670;
RA   Hellsten U., Harland R.M., Gilchrist M.J., Hendrix D., Jurka J.,
RA   Kapitonov V., Ovcharenko I., Putnam N.H., Shu S., Taher L., Blitz I.L.,
RA   Blumberg B., Dichmann D.S., Dubchak I., Amaya E., Detter J.C., Fletcher R.,
RA   Gerhard D.S., Goodstein D., Graves T., Grigoriev I.V., Grimwood J.,
RA   Kawashima T., Lindquist E., Lucas S.M., Mead P.E., Mitros T., Ogino H.,
RA   Ohta Y., Poliakov A.V., Pollet N., Robert J., Salamov A., Sater A.K.,
RA   Schmutz J., Terry A., Vize P.D., Warren W.C., Wells D., Wills A.,
RA   Wilson R.K., Zimmerman L.B., Zorn A.M., Grainger R., Grammer T.,
RA   Khokha M.K., Richardson P.M., Rokhsar D.S.;
RT   "The genome of the Western clawed frog Xenopus tropicalis.";
RL   Science 328:633-636(2010).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 38-646.
RC   TISSUE=Gastrula;
RG   Sanger Xenopus tropicalis EST/cDNA project;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Key component of inflammasomes that indirectly senses
CC       specific proteins from pathogenic bacteria and fungi and responds by
CC       assembling an inflammasome complex that promotes caspase-1 activation,
CC       cytokine production and macrophage pyroptosis. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
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DR   EMBL; AAMC01027550; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AAMC01027551; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; CR848622; CAJ83589.1; -; mRNA.
DR   AlphaFoldDB; F6R2G2; -.
DR   SMR; F6R2G2; -.
DR   STRING; 8364.ENSXETP00000018561; -.
DR   PaxDb; F6R2G2; -.
DR   eggNOG; ENOG502QWRJ; Eukaryota.
DR   InParanoid; F6R2G2; -.
DR   Proteomes; UP000008143; Genome assembly.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0042742; P:defense response to bacterium; IBA:GO_Central.
DR   GO; GO:0016045; P:detection of bacterium; IBA:GO_Central.
DR   GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR   GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR   GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR   Gene3D; 1.10.533.10; -; 1.
DR   Gene3D; 3.40.50.300; -; 1.
DR   Gene3D; 3.80.10.10; -; 1.
DR   InterPro; IPR001315; CARD.
DR   InterPro; IPR011029; DEATH-like_dom_sf.
DR   InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   InterPro; IPR007111; NACHT_NTPase.
DR   InterPro; IPR042220; NLRC4.
DR   InterPro; IPR040535; NLRC4_HD.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   PANTHER; PTHR47688; PTHR47688; 1.
DR   Pfam; PF00619; CARD; 1.
DR   Pfam; PF05729; NACHT; 1.
DR   Pfam; PF17889; NLRC4_HD; 1.
DR   SMART; SM00367; LRR_CC; 3.
DR   SUPFAM; SSF47986; SSF47986; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   PROSITE; PS50209; CARD; 1.
DR   PROSITE; PS50837; NACHT; 1.
PE   2: Evidence at transcript level;
KW   Apoptosis; ATP-binding; Cytoplasm; Immunity; Inflammatory response;
KW   Innate immunity; Leucine-rich repeat; Nucleotide-binding;
KW   Reference proteome; Repeat.
FT   CHAIN           1..997
FT                   /note="NLR family CARD domain-containing protein 4"
FT                   /id="PRO_0000419977"
FT   DOMAIN          1..88
FT                   /note="CARD"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00046"
FT   DOMAIN          165..478
FT                   /note="NACHT"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT   REPEAT          550..570
FT                   /note="LRR 1"
FT   REPEAT          629..652
FT                   /note="LRR 2"
FT   REPEAT          708..731
FT                   /note="LRR 3"
FT   REPEAT          735..758
FT                   /note="LRR 4"
FT   REPEAT          760..785
FT                   /note="LRR 5"
FT   REPEAT          797..820
FT                   /note="LRR 6"
FT   REPEAT          821..843
FT                   /note="LRR 7"
FT   REPEAT          851..875
FT                   /note="LRR 8"
FT   REPEAT          884..905
FT                   /note="LRR 9"
FT   REPEAT          908..935
FT                   /note="LRR 10"
FT   REPEAT          937..958
FT                   /note="LRR 11"
FT   REPEAT          972..994
FT                   /note="LRR 12"
FT   REGION          95..300
FT                   /note="Nucleotide-binding domain (NBD)"
FT                   /evidence="ECO:0000250"
FT   REGION          358..465
FT                   /note="Winged-helix domain (WHD)"
FT                   /evidence="ECO:0000250"
FT   BINDING         171..178
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT   CONFLICT        443
FT                   /note="Y -> F (in Ref. 2; CAJ83589)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   997 AA;  113626 MW;  463A3D3B238F89FF CRC64;
     MDLIRKNYAE LVQRMGRTIA VQITNDLFTR NILSMGEMEE ILSCKVAQDL TRELLNVILK
     KGTESCTRLL QSLENQDPFF YEDLIGQRVQ SGVTEEDLEN LADHLKRLYQ YPFFKKFNPL
     GEDTDIDIIF DLDRTFTDPL LWRKGTLNRR EKQLTLSEML EQLESPCVIE GEAGKGKTTI
     LKRIAMLWAS EKCRALADFK LVFFVTLRGA SEGLYETLSD QLFPITYSWN KKEFLNKIWH
     LGRKVLFLLD GYDEFQSESC TEIEELIKNN PKFNSTVIVS TRTETIGKLR RCGALIAETS
     DFSLDNAKQL IANVLEEDEA NGLLFQLEES SFMQNLMKTP LFVVIACALR MGESDFQMNT
     QTTLFSTLYD LMVEKKKYKI RHLSANILAA NIRKFGDLAL DGLFEQRFDF NEKHLSKIKE
     EVLLNIGLLN KYTAQRRKAV YRYFHTTFQE YIAGRRLSQL LSSEDNSDVT KGEDFLNKIV
     SVFDITTKYK NLLLYTCGSS KVATQKVVKH VAEVHKHDKN NYSTELVEFG LNLFFESSTK
     KELSQDFETL FSEKCLYINS HNISSHHIEF FLYLPNCLSA LQLIKLDLSG TFATVPSEST
     MDRDSKSAQS SVCDNYIPEK AVKLFFDWNQ SIQTLEVTLR DFHQLNKKDI KYLGKICCSA
     DSLRLNIKRS SGITGSLVGV LESCKNIQDL NVDSTKLSIE DERRIVQMTE MKTLSILNLH
     SEHLQGGLLE GLCNLVGLEK LVFHNIKIDK NDAKTLAEGI LSLKKLKRLS ISHISNIGDG
     MESIAESISL CCHELKELKL IDCCLSAKAL RSLGQSLYSL SHIEILDLSG NYLLEEGKES
     VEELAANLTH LDAIRTLMLP GGTDVKFCLE AVLPTLRRIP TLSELAFKRW NLTNDDLMTL
     ASYINSGFEN LSFLDLSDNC AQSAGWLSLT AILQYLPNLT YVNFSTEDLF TPDPDLVRKL
     VRAISALPLL HTMELNNWQL DDFDLAQIKK AKNMIHR