NLRC5_MOUSE
ID NLRC5_MOUSE Reviewed; 1915 AA.
AC C3VPR6; Q571H9;
DT 05-OCT-2010, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 2.
DT 03-AUG-2022, entry version 98.
DE RecName: Full=Protein NLRC5;
GN Name=Nlrc5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=BALB/cJ;
RX PubMed=20434986; DOI=10.1016/j.cell.2010.03.040;
RA Cui J., Zhu L., Xia X., Wang H.Y., Legras X., Hong J., Ji J., Shen P.,
RA Zheng S., Chen Z.J., Wang R.F.;
RT "NLRC5 negatively regulates the NF-kappaB and type I interferon signaling
RT pathways.";
RL Cell 141:483-496(2010).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 285-1915 (ISOFORM 2).
RC TISSUE=Spleen;
RX PubMed=12465718; DOI=10.1093/dnares/9.5.179;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Hara Y., Nagase T., Ohara O.,
RA Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: I.
RT The complete nucleotide sequences of 100 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 9:179-188(2002).
CC -!- FUNCTION: Probable regulator of the NF-kappa-B and type I interferon
CC signaling pathways. May also regulate the type II interferon signaling
CC pathway. Plays a role in homeostatic control of innate immunity and in
CC antiviral defense mechanisms. {ECO:0000269|PubMed:20434986}.
CC -!- SUBUNIT: Interacts with CHUK and IKBKB; prevents CHUK and IKBKB
CC phosphorylation and inhibits their kinase activity. Interacts with
CC DDX58 and IFIH1; blocks the interaction of MAVS to DDX58.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=C3VPR6-1; Sequence=Displayed;
CC Name=2;
CC IsoId=C3VPR6-2; Sequence=VSP_039702, VSP_039703;
CC -!- TISSUE SPECIFICITY: Expressed in spleen, thymus and lung.
CC {ECO:0000269|PubMed:20434986}.
CC -!- INDUCTION: By interferon gamma. By LPS and viruses (at protein level).
CC {ECO:0000269|PubMed:20434986}.
CC -!- MISCELLANEOUS: [Isoform 2]: Due to intron retention. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NLRP family. {ECO:0000305}.
CC -!- CAUTION: Supposed to contain a CARD domain at the N-terminus. However,
CC this domain is not detected by Pfam, PROSITE or SMART. Has a weak
CC similarity with a DAPIN domain. {ECO:0000305}.
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DR EMBL; FJ889356; ACP40992.1; -; mRNA.
DR EMBL; AK220210; BAD90135.1; -; mRNA.
DR CCDS; CCDS59741.1; -. [C3VPR6-1]
DR RefSeq; NP_001028379.2; NM_001033207.3. [C3VPR6-1]
DR RefSeq; XP_006531225.1; XM_006531162.2. [C3VPR6-1]
DR RefSeq; XP_006531226.1; XM_006531163.2. [C3VPR6-1]
DR RefSeq; XP_011246730.1; XM_011248428.1. [C3VPR6-1]
DR PDB; 2MJM; NMR; -; A=1-96.
DR PDBsum; 2MJM; -.
DR AlphaFoldDB; C3VPR6; -.
DR BMRB; C3VPR6; -.
DR SMR; C3VPR6; -.
DR IntAct; C3VPR6; 2.
DR STRING; 10090.ENSMUSP00000138322; -.
DR iPTMnet; C3VPR6; -.
DR PhosphoSitePlus; C3VPR6; -.
DR EPD; C3VPR6; -.
DR MaxQB; C3VPR6; -.
DR PeptideAtlas; C3VPR6; -.
DR PRIDE; C3VPR6; -.
DR ProteomicsDB; 293580; -. [C3VPR6-1]
DR ProteomicsDB; 293581; -. [C3VPR6-2]
DR Antibodypedia; 44042; 92 antibodies from 26 providers.
DR Ensembl; ENSMUST00000053085; ENSMUSP00000138322; ENSMUSG00000074151. [C3VPR6-1]
DR Ensembl; ENSMUST00000211816; ENSMUSP00000148677; ENSMUSG00000074151. [C3VPR6-1]
DR GeneID; 434341; -.
DR KEGG; mmu:434341; -.
DR UCSC; uc009mwj.2; mouse. [C3VPR6-2]
DR UCSC; uc012gio.1; mouse. [C3VPR6-1]
DR CTD; 84166; -.
DR MGI; MGI:3612191; Nlrc5.
DR VEuPathDB; HostDB:ENSMUSG00000074151; -.
DR eggNOG; KOG4308; Eukaryota.
DR GeneTree; ENSGT00940000160652; -.
DR HOGENOM; CLU_002522_1_0_1; -.
DR InParanoid; C3VPR6; -.
DR OMA; AAMVHMW; -.
DR OrthoDB; 19417at2759; -.
DR PhylomeDB; C3VPR6; -.
DR BioGRID-ORCS; 434341; 4 hits in 72 CRISPR screens.
DR ChiTaRS; Nlrc5; mouse.
DR PRO; PR:C3VPR6; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; C3VPR6; protein.
DR Bgee; ENSMUSG00000074151; Expressed in mesenteric lymph node and 85 other tissues.
DR ExpressionAtlas; C3VPR6; baseline and differential.
DR Genevisible; C3VPR6; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IDA:BHF-UCL.
DR GO; GO:0005634; C:nucleus; IDA:BHF-UCL.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; ISO:MGI.
DR GO; GO:0051607; P:defense response to virus; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IMP:UniProtKB.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:UniProtKB.
DR GO; GO:0060339; P:negative regulation of type I interferon-mediated signaling pathway; IDA:UniProtKB.
DR GO; GO:0060335; P:positive regulation of interferon-gamma-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0045345; P:positive regulation of MHC class I biosynthetic process; ISO:MGI.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; ISO:MGI.
DR GO; GO:0060340; P:positive regulation of type I interferon-mediated signaling pathway; ISS:UniProtKB.
DR GO; GO:0043549; P:regulation of kinase activity; ISO:MGI.
DR GO; GO:0009617; P:response to bacterium; IEP:MGI.
DR Gene3D; 3.40.50.300; -; 1.
DR Gene3D; 3.80.10.10; -; 7.
DR InterPro; IPR001611; Leu-rich_rpt.
DR InterPro; IPR006553; Leu-rich_rpt_Cys-con_subtyp.
DR InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR InterPro; IPR032675; LRR_dom_sf.
DR InterPro; IPR007111; NACHT_NTPase.
DR InterPro; IPR041210; NLRC5_atypical_Card.
DR InterPro; IPR041267; NLRP_HD2.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF18461; Atypical_Card; 1.
DR Pfam; PF13516; LRR_6; 8.
DR Pfam; PF05729; NACHT; 1.
DR Pfam; PF17776; NLRC4_HD2; 1.
DR SMART; SM00367; LRR_CC; 13.
DR SMART; SM00369; LRR_TYP; 9.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS50837; NACHT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; ATP-binding; Cytoplasm; Immunity;
KW Innate immunity; Leucine-rich repeat; Nucleotide-binding;
KW Reference proteome; Repeat.
FT CHAIN 1..1915
FT /note="Protein NLRC5"
FT /id="PRO_0000397867"
FT DOMAIN 223..542
FT /note="NACHT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT REPEAT 622..646
FT /note="LRR 1"
FT REPEAT 716..740
FT /note="LRR 2"
FT REPEAT 744..771
FT /note="LRR 3"
FT REPEAT 772..796
FT /note="LRR 4"
FT REPEAT 871..898
FT /note="LRR 5"
FT REPEAT 900..923
FT /note="LRR 6"
FT REPEAT 930..953
FT /note="LRR 7"
FT REPEAT 1006..1033
FT /note="LRR 8"
FT REPEAT 1034..1055
FT /note="LRR 9"
FT REPEAT 1138..1161
FT /note="LRR 10"
FT REPEAT 1162..1184
FT /note="LRR 11"
FT REPEAT 1240..1263
FT /note="LRR 12"
FT REPEAT 1265..1292
FT /note="LRR 13"
FT REPEAT 1348..1371
FT /note="LRR 14"
FT REPEAT 1481..1504
FT /note="LRR 15"
FT REPEAT 1519..1542
FT /note="LRR 16"
FT REPEAT 1552..1575
FT /note="LRR 17"
FT REPEAT 1576..1598
FT /note="LRR 18"
FT REPEAT 1603..1626
FT /note="LRR 19"
FT REPEAT 1631..1654
FT /note="LRR 20"
FT REPEAT 1659..1682
FT /note="LRR 21"
FT REPEAT 1687..1711
FT /note="LRR 22"
FT REPEAT 1715..1738
FT /note="LRR 23"
FT REPEAT 1741..1768
FT /note="LRR 24"
FT REPEAT 1769..1795
FT /note="LRR 25"
FT REPEAT 1821..1845
FT /note="LRR 26"
FT REPEAT 1849..1872
FT /note="LRR 27"
FT REGION 103..137
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 229..236
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00136"
FT VAR_SEQ 1384..1392
FT /note="LEEPWVDSV -> YLPALGRRV (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12465718"
FT /id="VSP_039702"
FT VAR_SEQ 1393..1915
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12465718"
FT /id="VSP_039703"
FT CONFLICT 489..490
FT /note="SF -> AS (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 578
FT /note="M -> T (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 641
FT /note="R -> C (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 646
FT /note="N -> K (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 833
FT /note="V -> I (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 881
FT /note="G -> R (in Ref. 1; ACP40992)"
FT /evidence="ECO:0000305"
FT CONFLICT 896
FT /note="A -> T (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 939
FT /note="N -> S (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT CONFLICT 1105
FT /note="R -> Q (in Ref. 2; BAD90135)"
FT /evidence="ECO:0000305"
FT HELIX 2..4
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 11..22
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 25..35
FT /evidence="ECO:0007829|PDB:2MJM"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 50..63
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 65..78
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 86..90
FT /evidence="ECO:0007829|PDB:2MJM"
FT HELIX 91..93
FT /evidence="ECO:0007829|PDB:2MJM"
SQ SEQUENCE 1915 AA; 211710 MW; 731919C80F353C1F CRC64;
MDAESIRLNN ENLWAWLVRL LSKNPEWLSA KLRSFLPTMD LDCSYEPSNP EVIHRQLNRL
FAQGMATWKS FINDLCFELD VPLDMEIPLV SIWGPRDEFS KQLGAGEESC PGPQLYHGAK
RPFQSYGSSP RRKNSKKQQL ELAKKYLKLL KTSAQQWHGG VCPGAWLTPH SPQTYIPPVL
QWSRATAPLD AQEGATLGDP EAADNIDVSI QDLFSFKAHK GPRVTVLLGK AGMGKTTLAY
RLRWRWAQGQ LDRFQALFLF EFRQLNMITQ LPTLPQLLFD LYLMPESEPD AVFQYLKENA
QEVLLIFDGL DEALHADSVG TDNAGSALTL FSELCHGNLL PGCWVMTTSR PGKLPSCVPT
EAATVHMWGF DGLRVEKYVT CFFSDLLSQE LALKEMRTNA RLRGMCAIPA LCTVTCFCLR
RLLPGSSPGQ SAALLPTITQ LYLQMVETFS PSETLLDTSI LGFGKVALRG LDTGKVVFSV
EDISPQLMSF GAVHSLLTSF CIHTRPGHEE IGYAFVHLSL QEFFAALYLM ASHTVDKDTL
VEYVTLNSHW VLRTKGRLGL SDHLPAFLAG LASHTCHMFL CQLAQQDRAW VGSRQAAVIQ
VLRKLASRKL TGPKMIELYH CVAETQDLEL ARFTAQSLPS RLSFHNFPLT HADLAALANI
LEHRDDPIHL DFDGCPLEPH CPEALVGCGQ VENLSFKSRK CGDAFAEALC RSLPTMGSLK
TLGLTGSRIT AQGISHLIQT LPLCSQLEEV SLHDNQLKDP EVLSLVELLP SLPKLQKLDL
SRNSFSRSIL LSLVKVAITC PTVRKLQVRE LDLIFYLSPV TETATQQSGA SDVQGKDSLK
EGQSRSLQLR LQKCQLRIRD AEALVELFQK SPQLEEVNLS GNHLEDDGCR LVAEAASQLH
IAQKLDLSDN GLSQTGVTYV LKAMSTCGTL EDLHISLLNN TVVLTFAQEP REQEGSCKGR
APLISFVSPV TSELSQRSRR IRLTHCGFLA KHTETLCEAL RASCQTHNLD HLDLSDNSLG
GKGVILLTEL LPGLGPLKSL NLSRNGLSMD AVFSLVQCLS SLQWVFHLDV SLESDCIFLR
GAGTSRDALE PKFQTGVQVL ELSQRYTSRS FCLQECQLEP TSLTFLCATL EKSPGPLEVQ
LSCKSLSDDS LKILLQCLPQ LPQLSLLQLR HTVLSSRSPF LLADIFNLCP RVRKVTLRSL
CHAVLHFDSN EEQEGVCCGF PGCSLSQEHM ETLCCALSKC NALSQLDLTD NLLGDIGLRC
LLECLPQLPI SGWLDLSHNN ISQEGILYLL ETLPSYPNIQ EVSVSLSSEQ IFRMCFSKKE
GAGTSLRLCE CSFSPEQVSK LASSLSQAQQ LTELWLTKCH LDLPQLTMLL NLVNRPTGLL
GLRLEEPWVD SVSLPALMEV CAQASGCLTE LSISEIQRKL WLQLEFPHQE GNSDSMALRL
AHCDLETEHS HLMIQLVETY ARLQQLSLSQ VSFNDNDGTS SKLLQNILLS SCELKSFRLT
FSQVSTKSLT HLAFGLGHCH HLEELDFSNN SLREEDTELL MGALQGTCRL KKLHLSFLPL
GASSLALLIQ GLSRMTLLQD LCLSHNQIGD VGTQCLAAIL PKLPELRKFD LSHNQIGDVG
TQCLAAILPK LPELRKFNLS HNQIGHVGTQ CLAAILPKLP ELRKFDLSRN QIGDVGTQCL
AAILPKLPEL RKFDLSGNRI GPAGGVQLVK SLTHFEHLEE IKLGNNALGE PTALELAQRL
PPQLRVLCLP SSHLGPEGAL GLAQALEQCP HIEEVSLAEN NLAGGVPRFS KRLPLLRQID
LEFCKIEDQA ARHLAANLTL FPALEKLLLS GNLLGDEVAA ELAQVLPQMG QLKKVNLEWN
RITARGAQLL AQGLVQGSCV PVIRLWNNPI LNDVAQSLQS QEPRLDFSIT DQQTL
