NLT2G_WHEAT
ID NLT2G_WHEAT Reviewed; 96 AA.
AC P82900; Q2PCC8;
DT 13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT 03-MAY-2011, sequence version 2.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Non-specific lipid-transfer protein 2G;
DE Short=LTP2G;
DE AltName: Full=7 kDa lipid transfer protein 1;
DE AltName: Full=Lipid transfer protein 2 isoform 1;
DE Short=LTP2-1;
DE Flags: Precursor;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000305};
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Leaf;
RX PubMed=16983534; DOI=10.1007/s00425-006-0397-7;
RA Boutrot F., Meynard D., Guiderdoni E., Joudrier P., Gautier M.-F.;
RT "The Triticum aestivum non-specific lipid transfer protein (TaLtp) gene
RT family: comparative promoter activity of six TaLtp genes in transgenic
RT rice.";
RL Planta 225:843-862(2007).
RN [2] {ECO:0000305}
RP PROTEIN SEQUENCE OF 30-96, FUNCTION, MASS SPECTROMETRY, AND DISULFIDE
RP BONDS.
RC TISSUE=Endosperm;
RX PubMed=11231292; DOI=10.1046/j.1432-1327.2001.02007.x;
RA Douliez J.-P., Pato C., Rabesona H., Molle D., Marion D.;
RT "Disulfide bond assignment, lipid transfer activity and secondary structure
RT of a 7-kDa plant lipid transfer protein, LTP2.";
RL Eur. J. Biochem. 268:1400-1403(2001).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.12 ANGSTROMS) OF 30-96 IN COMPLEX WITH
RP L-ALPHA-PALMITOYL-PHOSPHATIDYL GLYCEROL, AND DISULFIDE BONDS.
RX PubMed=15805594; DOI=10.1107/s0907444905000417;
RA Hoh F., Pons J.L., Gautier M.F., de Lamotte F., Dumas C.;
RT "Structure of a liganded type 2 non-specific lipid-transfer protein from
RT wheat and the molecular basis of lipid binding.";
RL Acta Crystallogr. D 61:397-406(2005).
CC -!- FUNCTION: Transfer lipids across membranes. May play a role in plant
CC defense or in the biosynthesis of cuticle layers.
CC {ECO:0000269|PubMed:11231292, ECO:0000303|PubMed:11231292}.
CC -!- SUBCELLULAR LOCATION: Secreted, cell wall {ECO:0000250}.
CC -!- MASS SPECTROMETRY: Mass=6971; Mass_error=1; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:11231292};
CC -!- SIMILARITY: Belongs to the plant LTP family. B11E subfamily.
CC {ECO:0000305}.
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DR EMBL; AJ852549; CAH69200.1; -; Genomic_DNA.
DR PDB; 1N89; NMR; -; A=30-96.
DR PDB; 1TUK; X-ray; 1.12 A; A=30-96.
DR PDBsum; 1N89; -.
DR PDBsum; 1TUK; -.
DR AlphaFoldDB; P82900; -.
DR SMR; P82900; -.
DR STRING; 4565.Traes_4BL_AB3EBF1FD.2; -.
DR Allergome; 8724; Tri a 7k-LTP.
DR PRIDE; P82900; -.
DR EnsemblPlants; TraesCAD_scaffold_031197_01G000500.1; TraesCAD_scaffold_031197_01G000500.1; TraesCAD_scaffold_031197_01G000500.
DR EnsemblPlants; TraesCLE_scaffold_005285_01G000700.1; TraesCLE_scaffold_005285_01G000700.1; TraesCLE_scaffold_005285_01G000700.
DR EnsemblPlants; TraesCS4B02G393300.1; TraesCS4B02G393300.1.cds1; TraesCS4B02G393300.
DR EnsemblPlants; TraesPAR_scaffold_002158_01G000300.1; TraesPAR_scaffold_002158_01G000300.1; TraesPAR_scaffold_002158_01G000300.
DR EnsemblPlants; TraesROB_scaffold_004343_01G000700.1; TraesROB_scaffold_004343_01G000700.1; TraesROB_scaffold_004343_01G000700.
DR EnsemblPlants; TraesWEE_scaffold_028928_01G000300.1; TraesWEE_scaffold_028928_01G000300.1; TraesWEE_scaffold_028928_01G000300.
DR Gramene; TraesCAD_scaffold_031197_01G000500.1; TraesCAD_scaffold_031197_01G000500.1; TraesCAD_scaffold_031197_01G000500.
DR Gramene; TraesCLE_scaffold_005285_01G000700.1; TraesCLE_scaffold_005285_01G000700.1; TraesCLE_scaffold_005285_01G000700.
DR Gramene; TraesCS4B02G393300.1; TraesCS4B02G393300.1.cds1; TraesCS4B02G393300.
DR Gramene; TraesPAR_scaffold_002158_01G000300.1; TraesPAR_scaffold_002158_01G000300.1; TraesPAR_scaffold_002158_01G000300.
DR Gramene; TraesROB_scaffold_004343_01G000700.1; TraesROB_scaffold_004343_01G000700.1; TraesROB_scaffold_004343_01G000700.
DR Gramene; TraesWEE_scaffold_028928_01G000300.1; TraesWEE_scaffold_028928_01G000300.1; TraesWEE_scaffold_028928_01G000300.
DR eggNOG; ENOG502S3N0; Eukaryota.
DR HOGENOM; CLU_158223_2_0_1; -.
DR OMA; TSCAMAI; -.
DR EvolutionaryTrace; P82900; -.
DR Proteomes; UP000019116; Unplaced.
DR Genevisible; P82900; TA.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:UniProtKB-KW.
DR CDD; cd01959; nsLTP2; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR033872; nsLTP2.
DR PANTHER; PTHR33214; PTHR33214; 1.
DR Pfam; PF14368; LTP_2; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell wall; Cell wall biogenesis/degradation;
KW Direct protein sequencing; Disulfide bond; Lipid transport; Lipid-binding;
KW Reference proteome; Secreted; Signal; Transport.
FT SIGNAL 1..29
FT /evidence="ECO:0000269|PubMed:11231292"
FT CHAIN 30..96
FT /note="Non-specific lipid-transfer protein 2G"
FT /id="PRO_0000153893"
FT DISULFID 31..63
FT /evidence="ECO:0000269|PubMed:11231292,
FT ECO:0000269|PubMed:15805594, ECO:0007744|PDB:1TUK"
FT DISULFID 39..53
FT /evidence="ECO:0000269|PubMed:11231292,
FT ECO:0000269|PubMed:15805594, ECO:0007744|PDB:1TUK"
FT DISULFID 54..89
FT /evidence="ECO:0000269|PubMed:11231292,
FT ECO:0000269|PubMed:15805594, ECO:0007744|PDB:1TUK"
FT DISULFID 65..96
FT /evidence="ECO:0000269|PubMed:11231292,
FT ECO:0000269|PubMed:15805594, ECO:0007744|PDB:1TUK"
FT HELIX 33..39
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 40..45
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 51..60
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 61..63
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 64..67
FT /evidence="ECO:0007829|PDB:1TUK"
FT TURN 71..73
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 74..77
FT /evidence="ECO:0007829|PDB:1TUK"
FT HELIX 80..88
FT /evidence="ECO:0007829|PDB:1TUK"
SQ SEQUENCE 96 AA; 9832 MW; 0016692D11293870 CRC64;
MAGMMKKQVV TALMLALVVL AAAPGGARAA CQASQLAVCA SAILSGAKPS GECCGNLRAQ
QGCFCQYAKD PTYGQYIRSP HARDTLTSCG LAVPHC
