NLTPX_ORYSJ
ID NLTPX_ORYSJ Reviewed; 96 AA.
AC Q10ST8; A3ADD5; B7E7J9; P83210; Q40631;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 22-AUG-2006, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=Non-specific lipid-transfer protein 2;
DE Short=nsLTP2;
DE AltName: Full=7 kDa lipid transfer protein;
DE Flags: Precursor;
GN Name=LTP-2; OrderedLocusNames=Os03g0111300, LOC_Os03g02050;
GN ORFNames=OsJ_008807;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16109971; DOI=10.1101/gr.3869505;
RG The rice chromosome 3 sequencing consortium;
RA Buell C.R., Yuan Q., Ouyang S., Liu J., Zhu W., Wang A., Maiti R., Haas B.,
RA Wortman J., Pertea M., Jones K.M., Kim M., Overton L., Tsitrin T.,
RA Fadrosh D., Bera J., Weaver B., Jin S., Johri S., Reardon M., Webb K.,
RA Hill J., Moffat K., Tallon L., Van Aken S., Lewis M., Utterback T.,
RA Feldblyum T., Zismann V., Iobst S., Hsiao J., de Vazeille A.R.,
RA Salzberg S.L., White O., Fraser C.M., Yu Y., Kim H., Rambo T., Currie J.,
RA Collura K., Kernodle-Thompson S., Wei F., Kudrna K., Ammiraju J.S.S.,
RA Luo M., Goicoechea J.L., Wing R.A., Henry D., Oates R., Palmer M.,
RA Pries G., Saski C., Simmons J., Soderlund C., Nelson W., de la Bastide M.,
RA Spiegel L., Nascimento L., Huang E., Preston R., Zutavern T., Palmer L.,
RA O'Shaughnessy A., Dike S., McCombie W.R., Minx P., Cordum H., Wilson R.,
RA Jin W., Lee H.R., Jiang J., Jackson S.;
RT "Sequence, annotation, and analysis of synteny between rice chromosome 3
RT and diverged grass species.";
RL Genome Res. 15:1284-1291(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP PROTEIN SEQUENCE OF 28-96, MASS SPECTROMETRY, AND DISULFIDE BONDS.
RC TISSUE=Seed;
RX PubMed=12056799; DOI=10.1016/s0006-291x(02)00509-0;
RA Liu Y.-J., Samuel D., Lin C.H., Lyu P.-C.;
RT "Purification and characterization of a novel 7-kDa non-specific lipid
RT transfer protein-2 from rice (Oryza sativa).";
RL Biochem. Biophys. Res. Commun. 294:535-540(2002).
RN [8]
RP STRUCTURE BY NMR OF 28-96, AND DISULFIDE BONDS.
RX PubMed=12011089; DOI=10.1074/jbc.m203113200;
RA Samuel D., Liu Y.-J., Cheng C.S., Lyu P.-C.;
RT "Solution structure of plant nonspecific lipid transfer protein-2 from rice
RT (Oryza sativa).";
RL J. Biol. Chem. 277:35267-35273(2002).
CC -!- FUNCTION: Transfer lipids across membranes. May play a role in plant
CC defense or in the biosynthesis of cuticle layers.
CC -!- MASS SPECTROMETRY: Mass=7001.8; Method=Electrospray;
CC Evidence={ECO:0000269|PubMed:12056799};
CC -!- SIMILARITY: Belongs to the plant LTP family. B11E subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAZ25324.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; DP000009; ABF93598.1; -; Genomic_DNA.
DR EMBL; AP008209; BAF10637.1; -; Genomic_DNA.
DR EMBL; AP014959; BAS81929.1; -; Genomic_DNA.
DR EMBL; CM000140; EAZ25324.1; ALT_INIT; Genomic_DNA.
DR EMBL; AK062506; BAG88346.1; -; mRNA.
DR RefSeq; XP_015629253.1; XM_015773767.1.
DR PDB; 1L6H; NMR; -; A=28-96.
DR PDBsum; 1L6H; -.
DR AlphaFoldDB; Q10ST8; -.
DR BMRB; Q10ST8; -.
DR SMR; Q10ST8; -.
DR STRING; 4530.OS03T0111300-01; -.
DR PaxDb; Q10ST8; -.
DR PRIDE; Q10ST8; -.
DR EnsemblPlants; Os03t0111300-01; Os03t0111300-01; Os03g0111300.
DR GeneID; 4331362; -.
DR Gramene; Os03t0111300-01; Os03t0111300-01; Os03g0111300.
DR KEGG; osa:4331362; -.
DR eggNOG; ENOG502S3N0; Eukaryota.
DR HOGENOM; CLU_158223_2_0_1; -.
DR InParanoid; Q10ST8; -.
DR OMA; VAGASCN; -.
DR OrthoDB; 1544606at2759; -.
DR EvolutionaryTrace; Q10ST8; -.
DR Proteomes; UP000000763; Chromosome 3.
DR Proteomes; UP000007752; Chromosome 3.
DR Proteomes; UP000059680; Chromosome 3.
DR Genevisible; Q10ST8; OS.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0006869; P:lipid transport; IEA:InterPro.
DR CDD; cd01959; nsLTP2; 1.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR033872; nsLTP2.
DR PANTHER; PTHR33214; PTHR33214; 1.
DR Pfam; PF14368; LTP_2; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Disulfide bond; Lipid-binding;
KW Reference proteome; Signal; Transport.
FT SIGNAL 1..27
FT /evidence="ECO:0000269|PubMed:12056799"
FT CHAIN 28..96
FT /note="Non-specific lipid-transfer protein 2"
FT /id="PRO_0000153892"
FT DISULFID 30..62
FT /evidence="ECO:0000269|PubMed:12011089,
FT ECO:0000269|PubMed:12056799, ECO:0007744|PDB:1L6H"
FT DISULFID 38..52
FT /evidence="ECO:0000269|PubMed:12011089,
FT ECO:0000269|PubMed:12056799, ECO:0007744|PDB:1L6H"
FT DISULFID 53..88
FT /evidence="ECO:0000269|PubMed:12011089,
FT ECO:0000269|PubMed:12056799, ECO:0007744|PDB:1L6H"
FT DISULFID 64..95
FT /evidence="ECO:0000269|PubMed:12011089,
FT ECO:0000269|PubMed:12056799, ECO:0007744|PDB:1L6H"
FT TURN 31..34
FT /evidence="ECO:0007829|PDB:1L6H"
FT HELIX 35..42
FT /evidence="ECO:0007829|PDB:1L6H"
FT HELIX 50..66
FT /evidence="ECO:0007829|PDB:1L6H"
FT HELIX 72..75
FT /evidence="ECO:0007829|PDB:1L6H"
FT TURN 77..80
FT /evidence="ECO:0007829|PDB:1L6H"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:1L6H"
SQ SEQUENCE 96 AA; 9535 MW; ADEDFC8C03372A6C CRC64;
MMRKLAVLVL AVAMVAACGG GVVGVAGAGC NAGQLTVCTG AIAGGARPTA ACCSSLRAQQ
GCFCQFAKDP RYGRYVNSPN ARKAVSSCGI ALPTCH
