NLTP_CORAV
ID NLTP_CORAV Reviewed; 115 AA.
AC Q9ATH2;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 87.
DE RecName: Full=Non-specific lipid-transfer protein Cor a 8.0101 {ECO:0000305};
DE Short=nsLTP Cor a 8 {ECO:0000303|PubMed:26417906};
DE AltName: Full=Allergen Cor a 8 {ECO:0000303|PubMed:14713920, ECO:0000303|PubMed:18036652, ECO:0000303|PubMed:19006093, ECO:0000303|PubMed:26417906, ECO:0000303|Ref.9};
DE AltName: Full=Lipid transfer protein Cor a 8 {ECO:0000303|PubMed:14713920, ECO:0000303|PubMed:18036652, ECO:0000303|PubMed:19006093};
DE Short=LTP Cor a 8 {ECO:0000303|PubMed:14713920, ECO:0000303|PubMed:18036652, ECO:0000303|PubMed:19006093, ECO:0000303|Ref.9};
DE AltName: Allergen=Cor a 8.0101 {ECO:0000305};
DE Flags: Precursor;
OS Corylus avellana (European hazel) (Corylus maxima).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Betulaceae; Corylus.
OX NCBI_TaxID=13451 {ECO:0000312|EMBL:AAK28533.1};
RN [1] {ECO:0000312|EMBL:AAK28533.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-46, TISSUE SPECIFICITY,
RP AND ALLERGEN.
RC STRAIN=cv. Piemonte {ECO:0000312|EMBL:AAK28533.1};
RC TISSUE=Seed {ECO:0000303|PubMed:14713920};
RX PubMed=14713920; DOI=10.1016/j.jaci.2003.09.013;
RA Schocker F., Luttkopf D., Scheurer S., Petersen A., Cistero-Bahima A.,
RA Enrique E., San Miguel-Moncin M., Akkerdaas J., van Ree R., Vieths S.,
RA Becker W.M.;
RT "Recombinant lipid transfer protein Cor a 8 from hazelnut: a new tool for
RT in vitro diagnosis of potentially severe hazelnut allergy.";
RL J. Allergy Clin. Immunol. 113:141-147(2004).
RN [2]
RP PROTEIN SEQUENCE OF 24-35, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE
RP SPECIFICITY, AND ALLERGEN.
RX PubMed=11898007; DOI=10.1067/mai.2002.121946;
RA Pastorello E.A., Vieths S., Pravettoni V., Farioli L., Trambaioli C.,
RA Fortunato D., Luettkopf D., Calamari M., Ansaloni R., Scibilia J.,
RA Ballmer-Weber B.K., Poulsen L.K., Wuetrich B., Hansen K.S., Robino A.M.,
RA Ortolani C., Conti A.;
RT "Identification of hazelnut major allergens in sensitive patients with
RT positive double-blind, placebo-controlled food challenge results.";
RL J. Allergy Clin. Immunol. 109:563-570(2002).
RN [3] {ECO:0007744|PDB:4XUW}
RP PROTEIN SEQUENCE OF 24-29, X-RAY CRYSTALLOGRAPHY (1.10 ANGSTROMS) OF
RP 24-115, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, MASS
RP SPECTROMETRY, DISULFIDE BONDS, AND CIRCULAR DICHROISM ANALYSIS.
RX PubMed=26417906; DOI=10.1021/acs.jafc.5b03534;
RA Offermann L.R., Bublin M., Perdue M.L., Pfeifer S., Dubiela P.,
RA Borowski T., Chruszcz M., Hoffmann-Sommergruber K.;
RT "Structural and Functional Characterization of the Hazelnut Allergen Cor a
RT 8.";
RL J. Agric. Food Chem. 63:9150-9158(2015).
RN [4]
RP PROTEIN SEQUENCE OF 43-53 AND 81-115, IDENTIFICATION BY MASS SPECTROMETRY,
RP SUBUNIT, TISSUE SPECIFICITY, ALLERGEN, AND CIRCULAR DICHROISM ANALYSIS.
RX PubMed=19006093; DOI=10.1002/mnfr.200800083;
RA Rigby N.M., Marsh J., Sancho A.I., Wellner K., Akkerdaas J., van Ree R.,
RA Knulst A., Fernandez-Rivas M., Brettlova V., Schilte P.P., Summer C.,
RA Pumphrey R., Shewry P.R., Mills E.N.;
RT "The purification and characterisation of allergenic hazelnut seed
RT proteins.";
RL Mol. Nutr. Food Res. 52:S251-S261(2008).
RN [5]
RP PROTEIN SEQUENCE OF 81-96, TISSUE SPECIFICITY, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND BIOTECHNOLOGY.
RX PubMed=21735061; DOI=10.1007/s00216-011-5218-6;
RA Ansari P., Stoppacher N., Baumgartner S.;
RT "Marker peptide selection for the determination of hazelnut by LC-MS/MS and
RT occurrence in other nuts.";
RL Anal. Bioanal. Chem. 402:2607-2615(2012).
RN [6]
RP PROTEIN SEQUENCE OF 81-96, TISSUE SPECIFICITY, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND BIOTECHNOLOGY.
RX PubMed=24577577; DOI=10.1007/s00216-014-7679-x;
RA Costa J., Ansari P., Mafra I., Oliveira M.B., Baumgartner S.;
RT "Assessing hazelnut allergens by protein- and DNA-based approaches: LC-
RT MS/MS, ELISA and real-time PCR.";
RL Anal. Bioanal. Chem. 406:2581-2590(2014).
RN [7]
RP TISSUE SPECIFICITY, AND ALLERGEN.
RX PubMed=18036652; DOI=10.1016/j.jaci.2007.10.009;
RA Flinterman A.E., Akkerdaas J.H., den Hartog Jager C.F., Rigby N.M.,
RA Fernandez-Rivas M., Hoekstra M.O., Bruijnzeel-Koomen C.A., Knulst A.C.,
RA van Ree R., Pasmans S.G.;
RT "Lipid transfer protein-linked hazelnut allergy in children from a non-
RT Mediterranean birch-endemic area.";
RL J. Allergy Clin. Immunol. 121:423-428(2008).
RN [8]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALLERGEN, AND 3D-STRUCTURE
RP MODELING.
RX PubMed=22616776; DOI=10.1186/2043-9113-2-12;
RA Lopez E., Cuadrado C., Burbano C., Jimenez M.A., Rodriguez J., Crespo J.F.;
RT "Effects of autoclaving and high pressure on allergenicity of hazelnut
RT proteins.";
RL J. Clin. Bioinforma. 2:12-12(2012).
RN [9]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX DOI=10.1111/ijfs.12079;
RA Garino C., Locatelli M., Coisson J.D., D'Andrea M., Cereti E.,
RA Travaglia F., Arlorio M.;
RT "Gene transcription analysis of hazelnut (C orylus avellana L.) allergens
RT Cor a 1, Cor a 8 and Cor a 11: a comparative study.";
RL Int. J. Food Sci. Technol. 48:1208-1217(2013).
CC -!- FUNCTION: Plant non-specific lipid-transfer proteins transfer
CC phospholipids as well as galactolipids across membranes. May play a
CC role in wax or cutin deposition in the cell walls of expanding
CC epidermal cells and certain secretory tissues.
CC {ECO:0000255|RuleBase:RU000628}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC pH dependence:
CC Stable at low pH. Refolds at pH 2.5 after thermal denaturation.
CC Thermal denaturation is irreversible at pH 7.5.
CC {ECO:0000269|PubMed:26417906};
CC Temperature dependence:
CC Resistant to heat (PubMed:26417906, PubMed:11898007). At room
CC temperature no effect in protein secondary structure by varying pH
CC (PubMed:26417906). {ECO:0000269|PubMed:11898007,
CC ECO:0000269|PubMed:26417906};
CC -!- SUBUNIT: Monomer. {ECO:0000269|PubMed:19006093}.
CC -!- TISSUE SPECIFICITY: Expressed in seed (at protein level)
CC (PubMed:11898007, PubMed:14713920, PubMed:18036652, PubMed:19006093,
CC PubMed:26417906, PubMed:21735061, PubMed:22616776, PubMed:24577577).
CC Expressed in seed (Ref.9). {ECO:0000269|PubMed:11898007,
CC ECO:0000269|PubMed:14713920, ECO:0000269|PubMed:18036652,
CC ECO:0000269|PubMed:19006093, ECO:0000269|PubMed:21735061,
CC ECO:0000269|PubMed:22616776, ECO:0000269|PubMed:24577577,
CC ECO:0000269|PubMed:26417906, ECO:0000269|Ref.9}.
CC -!- DEVELOPMENTAL STAGE: Expressed during seed maturation. Expressed at
CC three fruit developmental stages, at early stage (approximately 45 days
CC before harvest), at middle stage (approximately 30 days before harvest)
CC and at the final harvest stage. Higher level of expression before the
CC harvest, then decreasing as seeds get close to their ripeness point
CC (Ref.9). Expressed in raw seeds (PubMed:22616776).
CC {ECO:0000269|PubMed:22616776, ECO:0000269|Ref.9}.
CC -!- MASS SPECTROMETRY: Mass=9473.1; Method=MALDI; Note=Nonreduced protein.;
CC Evidence={ECO:0000269|PubMed:26417906};
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE of
CC patients allergic to hazelnuts (PubMed:14713920, PubMed:11898007,
CC PubMed:19006093, PubMed:18036652, PubMed:22616776). IgE-binding of the
CC natural protein is strongly reduced by autoclaving at 121 degrees
CC Celsius for 15 or 30 minutes or at 138 degrees Celsius for 15 or 30
CC minutes, but not by high pressure treatment alone (300 Mba, 400 Mba,
CC 500 Mba and 600 Mba) (PubMed:22616776). {ECO:0000269|PubMed:11898007,
CC ECO:0000269|PubMed:14713920, ECO:0000269|PubMed:18036652,
CC ECO:0000269|PubMed:19006093, ECO:0000269|PubMed:22616776}.
CC -!- BIOTECHNOLOGY: In order to protect patients that are allergic to
CC hazelnuts, it is extremely important to find ways to detect traces
CC amounts of hazelnut in foods that should not contain it for the food
CC labeling and safety purposes. This protein is used in the development
CC of these methods (PubMed:21735061, PubMed:24577577). For the detection
CC of hazelnut, a liquid chromatography tandem mass spectrometry (LC-
CC MS/MS) method in selected reaction monitoring (SRM) mode is developed
CC by using a selected marker peptide from this protein as standard
CC (PubMed:21735061). It is found that LC-MS/MS performs well in detecting
CC this peptide in prepared model chocolates spiked with hazelnut. The
CC sensitivity level is approximately 1 mg/kg (PubMed:24577577). The
CC transcription level of the gene encoding this protein is investigated
CC by a relative quantitative Real-Time PCR (RT-PCR) technique in order to
CC compare the transcript amounts between different cultivars, and in one
CC of the cultivars also in relation to different years of harvest and
CC ripening stages. Each hazelnut sample is classified by the Principal
CC Components Analysis (PCA) to better interpret the results. The method
CC may help in choosing hypoallergenic genotypes of hazelnut for both
CC growers and consumers (Ref.9). {ECO:0000269|PubMed:21735061,
CC ECO:0000269|PubMed:24577577, ECO:0000269|Ref.9}.
CC -!- SIMILARITY: Belongs to the plant LTP family. {ECO:0000255,
CC ECO:0000255|RuleBase:RU000628, ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF329829; AAK28533.1; -; mRNA.
DR PDB; 4XUW; X-ray; 1.10 A; A/B=24-115.
DR PDBsum; 4XUW; -.
DR AlphaFoldDB; Q9ATH2; -.
DR SMR; Q9ATH2; -.
DR Allergome; 245; Cor a 8.
DR Allergome; 3219; Cor a 8.0101.
DR GO; GO:0043245; C:extraorganismal space; IDA:UniProtKB.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0045735; F:nutrient reservoir activity; IDA:UniProtKB.
DR GO; GO:0006869; P:lipid transport; IEA:InterPro.
DR GO; GO:0048316; P:seed development; IEP:UniProtKB.
DR Gene3D; 1.10.110.10; -; 1.
DR InterPro; IPR036312; Bifun_inhib/LTP/seed_sf.
DR InterPro; IPR016140; Bifunc_inhib/LTP/seed_store.
DR InterPro; IPR000528; Plant_nsLTP.
DR PANTHER; PTHR33076; PTHR33076; 1.
DR Pfam; PF00234; Tryp_alpha_amyl; 1.
DR PRINTS; PR00382; LIPIDTRNSFER.
DR SMART; SM00499; AAI; 1.
DR SUPFAM; SSF47699; SSF47699; 1.
DR PROSITE; PS00597; PLANT_LTP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Direct protein sequencing; Disulfide bond;
KW Lipid-binding; Signal; Transport.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:11898007,
FT ECO:0000269|PubMed:14713920, ECO:0000269|PubMed:26417906"
FT CHAIN 24..115
FT /note="Non-specific lipid-transfer protein Cor a 8.0101"
FT /evidence="ECO:0000255, ECO:0000305|PubMed:11898007,
FT ECO:0000305|PubMed:14713920, ECO:0000305|PubMed:26417906"
FT /id="PRO_5004323179"
FT DISULFID 27..74
FT /evidence="ECO:0000269|PubMed:26417906,
FT ECO:0007744|PDB:4XUW"
FT DISULFID 37..51
FT /evidence="ECO:0000269|PubMed:26417906,
FT ECO:0007744|PDB:4XUW"
FT DISULFID 52..97
FT /evidence="ECO:0000269|PubMed:26417906,
FT ECO:0007744|PDB:4XUW"
FT DISULFID 72..111
FT /evidence="ECO:0000269|PubMed:26417906,
FT ECO:0007744|PDB:4XUW"
FT CONFLICT 24..25
FT /note="SL -> AI (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 27..34
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 35..37
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 38..43
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 49..61
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 65..80
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 87..96
FT /evidence="ECO:0007829|PDB:4XUW"
FT HELIX 111..113
FT /evidence="ECO:0007829|PDB:4XUW"
SQ SEQUENCE 115 AA; 11806 MW; 58BEA755360DEC2A CRC64;
MGSLKLVCAV LLCMMVAAPV ARASLTCPQI KGNLTPCVLY LKNGGVLPPS CCKGVRAVND
ASRTTSDRQS ACNCLKDTAK GIAGLNPNLA AGLPGKCGVN IPYKISPSTN CNNVK
