ID   NLTP_PEGHA              Reviewed;          20 AA.
AC   B3EWH4;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   16-MAY-2012, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Non-specific lipid-transfer protein PHP {ECO:0000303|PubMed:23165744};
DE            Short=LTP {ECO:0000250|UniProtKB:P82007};
DE            Short=nsLTP {ECO:0000250|UniProtKB:P82007};
DE   Flags: Fragment;
OS   Peganum harmala (Syrian rue) (Harmal peganum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Sapindales; Nitrariaceae; Peganum.
OX   NCBI_TaxID=43879;
RN   [1] {ECO:0000305}
RP   PROTEIN SEQUENCE, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
RC   TISSUE=Seed {ECO:0000303|PubMed:23165744};
RX   PubMed=23165744; DOI=10.1093/abbs/gms094;
RA   Ma X., Liu D., Tang H., Wang Y., Wu T., Li Y., Yang J., Yang J., Sun S.,
RA   Zhang F.;
RT   "Purification and characterization of a novel antifungal protein with
RT   antiproliferation and anti-HIV-1 reverse transcriptase activities from
RT   Peganum harmala seeds.";
RL   Acta Biochim. Biophys. Sin. 45:87-94(2013).
CC   -!- FUNCTION: Plant non-specific lipid-transfer proteins transfer
CC       phospholipids as well as galactolipids across membranes (By
CC       similarity). May play a role in wax or cutin deposition in the cell
CC       walls of expanding epidermal cells and certain secretory tissues (By
CC       similarity). Binds 1-pyrenedodecanoic fatty acid (PubMed:23165744). Has
CC       antifungal activity against P.digitatum (IC(50)=7.5 uM), A.alternata
CC       (IC(50)=1.5 uM), R.stolonifer (IC(50)=8.44 uM) and M.grisea
CC       (IC(50)=2.19 uM) (PubMed:23165744). Has no antibacterial activity
CC       (PubMed:23165744). {ECO:0000250|UniProtKB:P82007,
CC       ECO:0000269|PubMed:23165744}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       pH dependence:
CC         Stable between pH 4 and 10. {ECO:0000269|PubMed:23165744};
CC       Temperature dependence:
CC         Thermostable. Retains antifungal activity between 4 degrees Celsius
CC         and 60 degrees Celsius. Activity reduced after 30 min at 80 degrees
CC         Celsius. {ECO:0000269|PubMed:23165744};
CC   -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:23165744}.
CC   -!- SIMILARITY: Belongs to the plant LTP family. {ECO:0000255}.
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DR   AlphaFoldDB; B3EWH4; -.
DR   GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR   GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR   GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Antimicrobial; Direct protein sequencing; Fungicide; Lipid-binding;
KW   Transport.
FT   CHAIN           1..>20
FT                   /note="Non-specific lipid-transfer protein PHP"
FT                   /evidence="ECO:0000269|PubMed:23165744"
FT                   /id="PRO_0000417400"
FT   NON_TER         20
FT                   /evidence="ECO:0000303|PubMed:23165744"
SQ   SEQUENCE   20 AA;  2090 MW;  994A3682BCA23404 CRC64;
     ITCPQVTQSL APCVPYLISG