ID   NM111_KLULA             Reviewed;         985 AA.
AC   Q6CIT6;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   25-MAY-2022, entry version 94.
DE   RecName: Full=Pro-apoptotic serine protease NMA111;
DE            EC=3.4.21.-;
GN   Name=NMA111; OrderedLocusNames=KLLA0F24090g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Nuclear serine protease which mediates apoptosis.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR   EMBL; CR382126; CAG98861.1; -; Genomic_DNA.
DR   RefSeq; XP_456153.1; XM_456153.1.
DR   AlphaFoldDB; Q6CIT6; -.
DR   SMR; Q6CIT6; -.
DR   STRING; 28985.XP_456153.1; -.
DR   EnsemblFungi; CAG98861; CAG98861; KLLA0_F24090g.
DR   GeneID; 2895233; -.
DR   KEGG; kla:KLLA0_F24090g; -.
DR   eggNOG; KOG1421; Eukaryota.
DR   HOGENOM; CLU_003212_0_0_1; -.
DR   InParanoid; Q6CIT6; -.
DR   OMA; CVFDNHE; -.
DR   Proteomes; UP000000598; Chromosome F.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:EnsemblFungi.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0044255; P:cellular lipid metabolic process; IEA:EnsemblFungi.
DR   GO; GO:0034605; P:cellular response to heat; IEA:EnsemblFungi.
DR   GO; GO:0051438; P:regulation of ubiquitin-protein transferase activity; IEA:EnsemblFungi.
DR   GO; GO:0120174; P:stress-induced homeostatically regulated protein degradation pathway; IEA:EnsemblFungi.
DR   Gene3D; 2.30.42.10; -; 2.
DR   Gene3D; 2.40.10.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR025926; PDZ-like_dom.
DR   InterPro; IPR041489; PDZ_6.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR043504; Peptidase_S1_PA_chymotrypsin.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF12812; PDZ_1; 2.
DR   Pfam; PF17820; PDZ_6; 1.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SMART; SM00228; PDZ; 2.
DR   SUPFAM; SSF50156; SSF50156; 2.
DR   SUPFAM; SSF50494; SSF50494; 2.
PE   3: Inferred from homology;
KW   Apoptosis; Hydrolase; Nucleus; Protease; Reference proteome; Repeat;
KW   Serine protease.
FT   CHAIN           1..985
FT                   /note="Pro-apoptotic serine protease NMA111"
FT                   /id="PRO_0000320353"
FT   DOMAIN          287..385
FT                   /note="PDZ 1"
FT   DOMAIN          771..847
FT                   /note="PDZ 2"
FT   REGION          79..269
FT                   /note="Serine protease"
FT   ACT_SITE        117
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        148
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        231
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   985 AA;  109647 MW;  51B139AF5D4615FE CRC64;
     MTVELKRNLL ESSVEDIDLE SKKQRVDNDV DQNTSSVDED IDYEEDYDEV NDVPIDSGNS
     LFNNNAKWQE TISKVVKSVV SIHFSQVAPF DSEAALVSEA TGFIVDAKLG IILTNRHVVG
     PGPFIGYAIF DNHEECDVIP IYRDPVHDFG FLKFDPSKLK YIEVTALELK PSLAKVGSEI
     RVVGNDAGEK LSILSGFISR LDRNAPDYGE LTYNDFNTEY IQAAAAASGG SSGSPVITVE
     GYAVALQAGG SSEASTDFFL PLDRVVRALQ CVQTDKPITR GTIQTQWVLK PFDECRRLGL
     SEDREKEARK RFPGKTGLLV AEAILREGPA YNKVQEGDTL ISINGTPICS FIQVDNILDS
     SVGDEIIIVV QRGSKDISFR CTVGDLHIIT PSRYVEVCGA NFHELSYQMA RCYALPVKGV
     FVASATGSFD LDPKEKTGWV IDFVDNKETP TLDDFIEVMK TIPDEKRVIV KFHNLTDVQT
     QRITSVYIDR HWCSEFRIYT RNDKTGIWDY EKITEKLPPL PIKPQKAKIL DLPINDNKKL
     AKMSHSLCVV HSVIAITMDS MEPEPTNGCG LVLDAEKGYV IVSRSFVPHD CLNTFVSFAE
     SILVPAKVVF LHPTQNYAIV QYDPSLVDAP VCTPVLANER LERGDETNFI GYMYSNTLIS
     SKTKVNGISS LSVPNDIIPR YRATNLEVIS IDSNISTKCK SGILAADDGT VRAIWLSCMG
     NKEKLYLMGL DVVDIKEVVD ILRAGKNPKV SIVDAGFDSI SLLQARIRGV PEEWIKMMEE
     ESENRLQFIS VTRNSYTTIK ERLEPGDIIL SVNGKLVKRM RDIGGVVGTD DDTDIESELL
     FKVVRDAKVI DLKIKTVQIE ETTRIVVFAG CVLQAPHHAV RQVKTSIPSE VYAVSRGTSS
     LALQYGIEVT NFITHVNDQS TPDLDTFLKV VKEIPDNTYC KLRLMTFDDV PFAISLKTNY
     HYFPTVELRK NPKTGSWIEN DISET