ID   NM111_YEAS7             Reviewed;         997 AA.
AC   A6ZRW1; B0KZR3;
DT   26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Pro-apoptotic serine protease NMA111;
DE            EC=3.4.21.-;
DE   AltName: Full=111 kDa nuclear mediator of apoptosis;
GN   Name=NMA111; Synonyms=YNM3; ORFNames=SCY_4672;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=18780730; DOI=10.1534/genetics.108.092932;
RA   Sinha H., David L., Pascon R.C., Clauder-Muenster S., Krishnakumar S.,
RA   Nguyen M., Shi G., Dean J., Davis R.W., Oefner P.J., McCusker J.H.,
RA   Steinmetz L.M.;
RT   "Sequential elimination of major-effect contributors identifies additional
RT   quantitative trait loci conditioning high-temperature growth in yeast.";
RL   Genetics 180:1661-1670(2008).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Nuclear serine protease which mediates apoptosis through
CC       proteolysis of the apoptotic inhibitor BIR1. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with BIR1. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the peptidase S1C family. {ECO:0000305}.
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DR   EMBL; EF125227; ABN58634.1; -; Genomic_DNA.
DR   EMBL; AAFW02000067; EDN62693.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZRW1; -.
DR   SMR; A6ZRW1; -.
DR   PRIDE; A6ZRW1; -.
DR   EnsemblFungi; EDN62693; EDN62693; SCY_4672.
DR   HOGENOM; CLU_003212_0_0_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
DR   GO; GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 2.30.42.10; -; 2.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR025926; PDZ-like_dom.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR009003; Peptidase_S1_PA.
DR   InterPro; IPR001940; Peptidase_S1C.
DR   Pfam; PF00595; PDZ; 1.
DR   Pfam; PF12812; PDZ_1; 2.
DR   PRINTS; PR00834; PROTEASES2C.
DR   SMART; SM00228; PDZ; 2.
DR   SUPFAM; SSF50156; SSF50156; 3.
DR   SUPFAM; SSF50494; SSF50494; 2.
PE   3: Inferred from homology;
KW   Apoptosis; Hydrolase; Nucleus; Protease; Repeat; Serine protease.
FT   CHAIN           1..997
FT                   /note="Pro-apoptotic serine protease NMA111"
FT                   /id="PRO_0000320363"
FT   DOMAIN          300..378
FT                   /note="PDZ 1"
FT   DOMAIN          779..854
FT                   /note="PDZ 2"
FT   REGION          1..43
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          83..273
FT                   /note="Serine protease"
FT   ACT_SITE        121
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        152
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        235
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   997 AA;  110881 MW;  A26005C1DDDB932C CRC64;
     MTISLSNIKK RDHSKISDGT SGESSLVKRK QLESATGDQE EEYTDHEIII EPLHFANNNN
     TVLTDSENYL RWQNTISNVV KSVVSIHFSQ VAPFDCDSAL VSEATGFVVD AKLGIILTNR
     HVVGPGPFVG YVVFDNHEEC DVIPIYRDPV HDFGFLKFDP KNIKYSKIKA LTLKPSLAKV
     GSEIRVVGND AGEKLSILAG FISRIDRNAP EYGELTYNDF NTEYIQAAAS ASGGSSGSPV
     VNIDGYAVAL QAGGSTEAST DFFLPLDRIL RALICIQTNK PITRGTIQVQ WLLKPYDECR
     RLGLTSERES EARAKFPENI GLLVAETVLR EGPGYDKIKE GDTLISINGE TISSFMQVDK
     IQDENVGKEI QLVIQRGGVE CTVTCTVGDL HAITPHRYVE VCGATFHELS YQMARFYALP
     VRGVFLSSAS GSFNFDSKER VGWIVDSIDN KETPDLDTFI EIMKTIPDRK RVTVRYHHLT
     DQHSPLVTSI YIDRHWCNEF RVYTRNDTTG IWDYKNVADP LPADALKPRS AKIIPIPVNN
     EKVAKLSSSL CTVATMAAVP LDSLSADILK TSGLIIDAEK GYVLVSRRVV PHDCLDTFVT
     IADSLVVPAT VEFLHPTHNF AIVKYDPELV KAPLITPKLS TTRMKRGDKL QFIGFTQNDR
     IVTSETTVTD ISSVSIPSNL IPRYRATNLE AISIDCNVST RCNSGILTDN DGTVRGLWLP
     FLGERLENKE KVYLMGLDIM DCREVIDILK NGGKPRVSIV DAGFGSISVL QARIRGVPEE
     WIMRMEHESN NRLQFITVSR VSYTEDKIHL ETGDVILSVN GKLVTEMNDL NGVVSSADGI
     LPSAMLDFKV VRDGNIVDLK IKTVEVQETD RFVIFAGSIL QKPHHAVLQA MVDVPKGVYC
     TFRGESSPAL QYGISATNFI THVNEIETPD LDTFLKVVKT IPDNSYCKMR LMTFDNVPFA
     ISLKTNYHYF PTAELKRDNI THKWIEKEFT GNSQSEK