NMAT2_ARATH
ID NMAT2_ARATH Reviewed; 735 AA.
AC Q9FJR9; Q94JV3;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 123.
DE RecName: Full=Nuclear intron maturase 2, mitochondrial {ECO:0000303|PubMed:19946041};
DE Short=AtnMat2 {ECO:0000303|PubMed:19946041};
DE EC=3.1.-.-;
DE AltName: Full=Nuclear intron maturase 1 b {ECO:0000303|PubMed:12527773};
DE Short=AtnMat1b {ECO:0000303|PubMed:12527773};
DE Flags: Precursor;
GN Name=NMAT2 {ECO:0000303|PubMed:19946041};
GN Synonyms=NMAT1B {ECO:0000303|PubMed:12527773};
GN OrderedLocusNames=At5g46920 {ECO:0000312|Araport:AT5G46920};
GN ORFNames=MQD22.6 {ECO:0000312|EMBL:BAB10231.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 441-735.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY.
RX PubMed=12527773; DOI=10.1093/nar/gkg153;
RA Mohr G., Lambowitz A.M.;
RT "Putative proteins related to group II intron reverse
RT transcriptase/maturases are encoded by nuclear genes in higher plants.";
RL Nucleic Acids Res. 31:647-652(2003).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, SUBUNIT, GENE FAMILY,
RP AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=19946041; DOI=10.1261/rna.1776409;
RA Keren I., Bezawork-Geleta A., Kolton M., Maayan I., Belausov E., Levy M.,
RA Mett A., Gidoni D., Shaya F., Ostersetzer-Biran O.;
RT "AtnMat2, a nuclear-encoded maturase required for splicing of group-II
RT introns in Arabidopsis mitochondria.";
RL RNA 15:2299-2311(2009).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=22429648; DOI=10.1111/j.1365-313x.2012.04998.x;
RA Keren I., Tal L., des Francs-Small C.C., Araujo W.L., Shevtsov S.,
RA Shaya F., Fernie A.R., Small I., Ostersetzer-Biran O.;
RT "nMAT1, a nuclear-encoded maturase involved in the trans-splicing of nad1
RT intron 1, is essential for mitochondrial complex I assembly and function.";
RL Plant J. 71:413-426(2012).
RN [7]
RP REVIEW ON SPLICING FACTORS.
RC STRAIN=cv. Columbia;
RX PubMed=24600456; DOI=10.3389/fpls.2014.00035;
RA Brown G.G., Colas des Francs-Small C., Ostersetzer-Biran O.;
RT "Group II intron splicing factors in plant mitochondria.";
RL Front. Plant Sci. 5:35-35(2014).
CC -!- FUNCTION: Nuclear-encoded maturase required for splicing of group-II
CC introns in mitochondria. Involved in the splicing of mitochondrial
CC COX2, NAD1 and NAD7 transcripts (PubMed:19946041). Necessary for
CC mitochondrial biogenesis during early developmental stages
CC (PubMed:22429648). {ECO:0000269|PubMed:19946041,
CC ECO:0000269|PubMed:22429648}.
CC -!- SUBUNIT: Associated to a large ribonucleoprotein complex in
CC mitochondria containing group-II intron RNAs.
CC {ECO:0000269|PubMed:19946041}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:19946041}.
CC -!- DISRUPTION PHENOTYPE: Low germination, slow growth, and late flowering,
CC as well as partial sterile of both male and female gametes. Small, pale
CC and round-shaped leaves. Impaired splicing of mitochondrial group-II
CC introns-containing COX2, NAD1 and NAD7 transcripts (PubMed:19946041).
CC Abnormal mitochondrial mor-phology, including low-density cristae
CC (PubMed:22429648). {ECO:0000269|PubMed:19946041,
CC ECO:0000269|PubMed:22429648}.
CC -!- SIMILARITY: Belongs to the plant nuclear intron maturase (nMat) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK49635.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB013394; BAB10231.1; -; Genomic_DNA.
DR EMBL; CP002688; AED95448.1; -; Genomic_DNA.
DR EMBL; AF372919; AAK49635.1; ALT_INIT; mRNA.
DR EMBL; BT003034; AAO23599.1; -; mRNA.
DR RefSeq; NP_199503.1; NM_124063.3.
DR AlphaFoldDB; Q9FJR9; -.
DR STRING; 3702.AT5G46920.1; -.
DR PaxDb; Q9FJR9; -.
DR PRIDE; Q9FJR9; -.
DR ProteomicsDB; 249444; -.
DR EnsemblPlants; AT5G46920.1; AT5G46920.1; AT5G46920.
DR GeneID; 834738; -.
DR Gramene; AT5G46920.1; AT5G46920.1; AT5G46920.
DR KEGG; ath:AT5G46920; -.
DR Araport; AT5G46920; -.
DR TAIR; locus:2170992; AT5G46920.
DR eggNOG; KOG1075; Eukaryota.
DR HOGENOM; CLU_009993_1_0_1; -.
DR InParanoid; Q9FJR9; -.
DR OMA; LPSWGHC; -.
DR OrthoDB; 356246at2759; -.
DR PhylomeDB; Q9FJR9; -.
DR PRO; PR:Q9FJR9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJR9; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0000373; P:Group II intron splicing; IMP:UniProtKB.
DR GO; GO:0006314; P:intron homing; IEA:UniProtKB-KW.
DR GO; GO:0090615; P:mitochondrial mRNA processing; IMP:UniProtKB.
DR GO; GO:0007005; P:mitochondrion organization; IMP:UniProtKB.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR024937; Domain_X.
DR Pfam; PF01348; Intron_maturas2; 1.
DR SUPFAM; SSF56672; SSF56672; 1.
PE 1: Evidence at protein level;
KW Endonuclease; Hydrolase; Intron homing; Mitochondrion; Nuclease;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..12
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 13..735
FT /note="Nuclear intron maturase 2, mitochondrial"
FT /id="PRO_0000440120"
FT DOMAIN 161..460
FT /note="Reverse transcriptase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00405"
FT REGION 485..653
FT /note="Intron maturase type-2"
FT /evidence="ECO:0000255"
FT REGION 707..735
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 713..735
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 735 AA; 85180 MW; 3C4A940CDA6E1590 CRC64;
MRRSFSVLGP YKWLKPSSTY TNVYCFVTNP PLISNPNNGS AIFRYLSYFA PQQRRQQQQA
PDPDDPANLL KEDGVSLCSQ MWLENFKEPD KTATNLTSYL RRFELWVLAY QKVCCDELGA
YVPRSSIQRS ALENLLALRN SVLDDRFKWG SRLDFYIKSP RDKTDYESLS KRKIKAILTT
TQPTPFQDRI VQEVLLMILE PIYESRFSQK SFAFRPGRTA HTVLRVIRRN FAGYLWYVKG
DLSVVLDGMK VGFVISSLMR DVRDKKVIDL IKSALVTPVV TSKVEDGEKK KTKKRKYQKK
RVLAEDEPKP DPYWLETFFG FAPEEAGKSP QWGHCGILSP LLVNVCLDEL DRWMETKVKD
FYRPSKSDVI WNNPEGEADQ GNTSWPEFVP TSGPDKTRKM DYVRYGGHIL IGVRGPRADA
ATLRKELIEF VDQKYMLRLD NENLPIEHIT KGIMFLDHVL CRRVVYPTLR YTATGGKIIS
EKGVGTLLSV TASLKQCIKQ FRKLLFIKGD RDPDPQPCFR MFHATQAHTN NQMNKFLTTI
AEWYRFADNR KKIVNFCSYI IRGSLAKLYA AKYKLRSRAK VYKFANRNLS LPLLQKKGQS
PEYQNLLRMG LAESVDGLVY TRMSLVPETD YSPFPGNWRP EHEKFLIEYL TLDEPKTLEE
QKRFIREKGL VSPQDYTSML VWNYKRNAIP MDQVSILKDQ PFLLGSSSTY NRDNDDQKNK
EEDEDSEDGL RIARM
