NMAT3_ARATH
ID NMAT3_ARATH Reviewed; 723 AA.
AC Q9LZA5; F4KI46;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2017, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Nuclear intron maturase 3, mitochondrial {ECO:0000303|PubMed:19946041};
DE Short=AtnMat3 {ECO:0000303|PubMed:19946041};
DE EC=3.1.-.-;
DE AltName: Full=Nuclear intron maturase 2 a {ECO:0000303|PubMed:12527773};
DE Short=AtnMat2a {ECO:0000303|PubMed:12527773};
DE Flags: Precursor;
GN Name=NMAT3 {ECO:0000303|PubMed:19946041};
GN Synonyms=NMAT2A {ECO:0000303|PubMed:12527773};
GN OrderedLocusNames=At5g04050 {ECO:0000312|Araport:AT5G04050};
GN ORFNames=F8F6.260 {ECO:0000312|EMBL:CAB85525.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12527773; DOI=10.1093/nar/gkg153;
RA Mohr G., Lambowitz A.M.;
RT "Putative proteins related to group II intron reverse
RT transcriptase/maturases are encoded by nuclear genes in higher plants.";
RL Nucleic Acids Res. 31:647-652(2003).
RN [4]
RP SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=19946041; DOI=10.1261/rna.1776409;
RA Keren I., Bezawork-Geleta A., Kolton M., Maayan I., Belausov E., Levy M.,
RA Mett A., Gidoni D., Shaya F., Ostersetzer-Biran O.;
RT "AtnMat2, a nuclear-encoded maturase required for splicing of group-II
RT introns in Arabidopsis mitochondria.";
RL RNA 15:2299-2311(2009).
RN [5]
RP REVIEW ON SPLICING FACTORS.
RC STRAIN=cv. Columbia;
RX PubMed=24600456; DOI=10.3389/fpls.2014.00035;
RA Brown G.G., Colas des Francs-Small C., Ostersetzer-Biran O.;
RT "Group II intron splicing factors in plant mitochondria.";
RL Front. Plant Sci. 5:35-35(2014).
CC -!- FUNCTION: Nuclear-encoded maturase required for splicing of group-II
CC introns in mitochondria. Necessary for mitochondrial biogenesis during
CC early developmental stages. {ECO:0000250|UniProtKB:Q9CA78}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:19946041}.
CC -!- SIMILARITY: Belongs to the plant nuclear intron maturase (nMat) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AED90691.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB85525.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL162873; CAB85525.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED90691.1; ALT_SEQ; Genomic_DNA.
DR PIR; T48432; T48432.
DR RefSeq; NP_001154695.1; NM_001161223.1.
DR AlphaFoldDB; Q9LZA5; -.
DR SMR; Q9LZA5; -.
DR STRING; 3702.AT5G04050.2; -.
DR PRIDE; Q9LZA5; -.
DR ProteomicsDB; 251183; -.
DR GeneID; 830284; -.
DR KEGG; ath:AT5G04050; -.
DR Araport; AT5G04050; -.
DR TAIR; locus:2150660; AT5G04050.
DR eggNOG; KOG1075; Eukaryota.
DR OrthoDB; 274919at2759; -.
DR PhylomeDB; Q9LZA5; -.
DR PRO; PR:Q9LZA5; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LZA5; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0000373; P:Group II intron splicing; ISS:UniProtKB.
DR GO; GO:0006315; P:homing of group II introns; IBA:GO_Central.
DR GO; GO:0090615; P:mitochondrial mRNA processing; ISS:UniProtKB.
DR GO; GO:0008380; P:RNA splicing; IMP:TAIR.
PE 3: Inferred from homology;
KW DNA-binding; Endonuclease; Hydrolase; Intron homing; Metal-binding;
KW Mitochondrion; Nuclease; Nucleotidyltransferase; Reference proteome;
KW RNA-directed DNA polymerase; Transferase; Transit peptide; Zinc;
KW Zinc-finger.
FT TRANSIT 1..26
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 27..723
FT /note="Nuclear intron maturase 3, mitochondrial"
FT /id="PRO_0000440121"
FT ZN_FING 646..700
FT /note="THAP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00309"
FT REGION 532..597
FT /note="Intron maturase type-2; degenerate"
FT /evidence="ECO:0000255"
SQ SEQUENCE 723 AA; 83496 MW; 9B2E5E5F37BD9F9C CRC64;
MVLRLRVHSF YNRGISFLVS SSLRNLSTAS SLFLNSDQTI TEPLVKSELE ALVLKQYSHG
KFYSLVKNAV SLPCVLLAAC QNLSLSANSS GDLADRVSRR FSIEEMGREI REGRFDIRSC
CVEFISSSLV LPNLKLKVLI EAIRMVLEIV YDDRFATFSY GGRVGMGRHT AIRYLKNSVE
NPRWWFRVSF AREMFEERNV DILCGFVGEK INDVMLIEMI KKLFEFGILK IELGGCNSGR
GFPQECGLCS ILINVYFDGL DKEIQDLRLK MKVKNPRVGT GDEESTGNVF FKPVNIYAVR
YLDEILVITS GSKMLTMDLK KRIVDILEQR LELRVDRLNT SIHSAVSEKI NFLGMYLQAV
PPSVLRPPKS EKAVRAMKKY QRQKDVRKLE LRNARERNRK TLGLKIFRHV LKKIKQSNGF
KFEGEIENEV RDIFQSWGEE VMQDFMGSLE ERWKWHWLLT RGDFLSLRHI REKLPQDLID
AYDEFQEQVD KHLAPTQAKK VLEDEERRVE EEEEQRYAER TVEDLTKLCM KVSAPEELVR
KAIKLVGFTN SMGRPRPIIH LVTLEDSDII KWYARHEKHG STKKLIRHYT KDLRVSDLDG
REEAHFPSER EVKMMGDKNL SDPKPVDGTL SLLLIRLASD EPLHHCAASF CERSDTIMHR
VHLLQNRLHI NPLDEEKWVP GMGTIHSALN RKCLPLCSTH ISDVYLGKIT LQDVDSSSFI
DLR
