NMAT4_ARATH
ID NMAT4_ARATH Reviewed; 798 AA.
AC Q9CA78;
DT 07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT 07-JUN-2017, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Nuclear intron maturase 4, mitochondrial {ECO:0000303|PubMed:19946041};
DE Short=AtnMat4 {ECO:0000303|PubMed:19946041};
DE EC=3.1.-.-;
DE AltName: Full=Nuclear intron maturase 2 b {ECO:0000303|PubMed:12527773};
DE Short=AtnMat2b {ECO:0000303|PubMed:12527773};
DE Flags: Precursor;
GN Name=NMAT4 {ECO:0000303|PubMed:19946041};
GN Synonyms=NMAT2B {ECO:0000303|PubMed:12527773};
GN OrderedLocusNames=At1g74350 {ECO:0000312|Araport:AT1G74350};
GN ORFNames=F1M20.3 {ECO:0000312|EMBL:AAG52355.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY.
RX PubMed=12527773; DOI=10.1093/nar/gkg153;
RA Mohr G., Lambowitz A.M.;
RT "Putative proteins related to group II intron reverse
RT transcriptase/maturases are encoded by nuclear genes in higher plants.";
RL Nucleic Acids Res. 31:647-652(2003).
RN [4]
RP SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RX PubMed=19946041; DOI=10.1261/rna.1776409;
RA Keren I., Bezawork-Geleta A., Kolton M., Maayan I., Belausov E., Levy M.,
RA Mett A., Gidoni D., Shaya F., Ostersetzer-Biran O.;
RT "AtnMat2, a nuclear-encoded maturase required for splicing of group-II
RT introns in Arabidopsis mitochondria.";
RL RNA 15:2299-2311(2009).
RN [5]
RP REVIEW ON SPLICING FACTORS.
RC STRAIN=cv. Columbia;
RX PubMed=24600456; DOI=10.3389/fpls.2014.00035;
RA Brown G.G., Colas des Francs-Small C., Ostersetzer-Biran O.;
RT "Group II intron splicing factors in plant mitochondria.";
RL Front. Plant Sci. 5:35-35(2014).
RN [6]
RP FUNCTION, DISRUPTION PHENOTYPE, ALTERNATIVE SPLICING, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=24506473; DOI=10.1111/tpj.12466;
RA Cohen S., Zmudjak M., Colas des Francs-Small C., Malik S., Shaya F.,
RA Keren I., Belausov E., Many Y., Brown G.G., Small I., Ostersetzer-Biran O.;
RT "nMAT4, a maturase factor required for nad1 pre-mRNA processing and
RT maturation, is essential for holocomplex I biogenesis in Arabidopsis
RT mitochondria.";
RL Plant J. 78:253-268(2014).
CC -!- FUNCTION: Nuclear-encoded maturase required for splicing of group-II
CC introns in mitochondria. Involved in NAD1 pre-mRNA processing and
CC maturation of introns 1, 3 and 4. Necessary for mitochondrial
CC biogenesis during early developmental stages. Essential for respiratory
CC holocomplex I biogenesis in mitochondria.
CC {ECO:0000269|PubMed:24506473}.
CC -!- SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion
CC {ECO:0000269|PubMed:19946041, ECO:0000269|PubMed:24506473}.
CC -!- SUBCELLULAR LOCATION: [Isoform 2]: Plastid, chloroplast
CC {ECO:0000269|PubMed:19946041, ECO:0000269|PubMed:24506473}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative initiation; Named isoforms=2;
CC Name=1; Synonyms=long {ECO:0000303|PubMed:19946041}, nMAT4L
CC {ECO:0000303|PubMed:24506473};
CC IsoId=Q9CA78-1; Sequence=Displayed;
CC Name=2; Synonyms=short {ECO:0000303|PubMed:19946041}, nMAT4S
CC {ECO:0000303|PubMed:24506473};
CC IsoId=Q9CA78-2; Sequence=VSP_058948;
CC -!- DISRUPTION PHENOTYPE: Altered mitochondrial morphology. Impaired seed
CC germination (e.g. many dark and wrinkled seeds in siliques), seedling
CC establishment and development (e.g. abnormal primary root elongation
CC and vegetative growth). Defects in the processing and maturation of
CC various mitochondrial NAD1 introns. Modified respiration phenotypes
CC associated with complex I defects. Seed-specific deficiency in the
CC regulation of carbohydrate metabolism. Small plants requiring sucrose-
CC supplemented medium to survive. {ECO:0000269|PubMed:24506473}.
CC -!- SIMILARITY: Belongs to the plant nuclear intron maturase (nMat) family.
CC {ECO:0000305}.
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DR EMBL; AC011765; AAG52355.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35581.1; -; Genomic_DNA.
DR PIR; B96772; B96772.
DR RefSeq; NP_177575.1; NM_106095.2.
DR AlphaFoldDB; Q9CA78; -.
DR STRING; 3702.AT1G74350.1; -.
DR PaxDb; Q9CA78; -.
DR PeptideAtlas; Q9CA78; -.
DR PRIDE; Q9CA78; -.
DR ProteomicsDB; 251066; -. [Q9CA78-1]
DR EnsemblPlants; AT1G74350.1; AT1G74350.1; AT1G74350.
DR GeneID; 843776; -.
DR Gramene; AT1G74350.1; AT1G74350.1; AT1G74350.
DR KEGG; ath:AT1G74350; -.
DR Araport; AT1G74350; -.
DR TAIR; locus:2019140; AT1G74350.
DR eggNOG; KOG1075; Eukaryota.
DR HOGENOM; CLU_013547_0_0_1; -.
DR InParanoid; Q9CA78; -.
DR OrthoDB; 274919at2759; -.
DR PhylomeDB; Q9CA78; -.
DR PRO; PR:Q9CA78; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CA78; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0003964; F:RNA-directed DNA polymerase activity; IBA:GO_Central.
DR GO; GO:0000373; P:Group II intron splicing; IMP:UniProtKB.
DR GO; GO:0006315; P:homing of group II introns; IBA:GO_Central.
DR GO; GO:0090615; P:mitochondrial mRNA processing; IMP:UniProtKB.
DR GO; GO:1900864; P:mitochondrial RNA modification; IMP:TAIR.
DR GO; GO:0007005; P:mitochondrion organization; IMP:UniProtKB.
DR GO; GO:0032885; P:regulation of polysaccharide biosynthetic process; IMP:UniProtKB.
DR GO; GO:0009845; P:seed germination; IMP:TAIR.
DR GO; GO:0090351; P:seedling development; IMP:TAIR.
DR InterPro; IPR024937; Domain_X.
DR InterPro; IPR000477; RT_dom.
DR Pfam; PF01348; Intron_maturas2; 1.
DR Pfam; PF00078; RVT_1; 1.
PE 3: Inferred from homology;
KW Alternative initiation; Chloroplast; DNA-binding; Endonuclease; Hydrolase;
KW Intron homing; Metal-binding; Mitochondrion; Nuclease; Plastid;
KW Reference proteome; Transit peptide; Zinc; Zinc-finger.
FT TRANSIT 1..16
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 17..798
FT /note="Nuclear intron maturase 4, mitochondrial"
FT /id="PRO_0000440122"
FT ZN_FING 729..778
FT /note="THAP-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00309"
FT REGION 578..665
FT /note="Intron maturase type-2"
FT /evidence="ECO:0000255"
FT VAR_SEQ 46..90
FT /note="Missing (in isoform 2)"
FT /id="VSP_058948"
SQ SEQUENCE 798 AA; 90268 MW; EE646E421744A0D5 CRC64;
MFRKRNLVLD LLRRCYIETL IPRGLWSRTI STFPKSALLA TDNAEMASKE TGMFSLAGEL
ASLVEESSSH VDDDSKPRSR MELKRSLELR LKKRVKEQCI NGKFSDLLKK VIARPETLRD
AYDCIRLNSN VSITERNGSV AFDSIAEELS SGVFDVASNT FSIVARDKTK EVLVLPSVAL
KVVQEAIRIV LEVVFSPHFS KISHSCRSGR GRASALKYIN NNISRSDWCF TLSLNKKLDV
SVFENLLSVM EEKVEDSSLS ILLRSMFEAR VLNLEFGGFP KGHGLPQEGV LSRVLMNIYL
DRFDHEFYRI SMRHEALGLD SKTDEDSPGS KLRSWFRRQA GEQGLKSTTE QDVALRVYCC
RFMDEIYFSV SGPKKVASDI RSEAIGFLRN SLHLDITDET DPSPCEATSG LRVLGTLVRK
NVRESPTVKA VHKLKEKVRL FALQKEEAWT LGTVRIGKKW LGHGLKKVKE SEIKGLADSN
STLSQISCHR KAGMETDHWY KILLRIWMED VLRTSADRSE EFVLSKHVVE PTVPQELRDA
FYKFQNAAAA YVSSETANLE ALLPCPQSHD RPVFFGDVVA PTNAIGRRLY RYGLITAKGY
ARSNSMLILL DTAQIIDWYS GLVRRWVIWY EGCSNFDEIK ALIDNQIRMS CIRTLAAKYR
IHENEIEKRL DLELSTIPSA EDIEQEIQHE KLDSPAFDRD EHLTYGLSNS GLCLLSLARL
VSESRPCNCF VIGCSMAAPA VYTLHAMERQ KFPGWKTGFS VCIPSSLNGR RIGLCKQHLK
DLYIGQISLQ AVDFGAWR
