NMB_MOUSE
ID NMB_MOUSE Reviewed; 121 AA.
AC Q9CR53;
DT 11-FEB-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Neuromedin-B;
DE Contains:
DE RecName: Full=Neuromedin-B-32;
DE Contains:
DE RecName: Full=Neuromedin-B;
DE Flags: Precursor;
GN Name=Nmb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Embryonic head;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=26855425; DOI=10.1038/nature16964;
RA Li P., Janczewski W.A., Yackle K., Kam K., Pagliardini S., Krasnow M.A.,
RA Feldman J.L.;
RT "The peptidergic control circuit for sighing.";
RL Nature 530:293-297(2016).
RN [4]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=28780306; DOI=10.1016/j.yexcr.2017.08.003;
RA Yeo C.E., Kang W.Y., Seong S.J., Cho S., Lee H.W., Yoon Y.R., Kim H.J.;
RT "Neuromedin B and its receptor silencing suppresses osteoclast generation
RT by modulating precursor proliferation via M-CSF/c-Fms/D-type cyclins.";
RL Exp. Cell Res. 359:112-119(2017).
RN [5]
RP FUNCTION.
RX PubMed=30734045; DOI=10.2340/00015555-3143;
RA Ehling S., Fukuyama T., Ko M.C., Olivry T., Baeumer W.;
RT "Neuromedin B Induces Acute Itch in Mice via the Activation of Peripheral
RT Sensory Neurons.";
RL Acta Derm. Venereol. 99:587-593(2019).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=31601264; DOI=10.1186/s13567-019-0695-2;
RA Yang G., Huang H., Tang M., Cai Z., Huang C., Qi B., Chen J.L.;
RT "Role of neuromedin B and its receptor in the innate immune responses
RT against influenza A virus infection in vitro and in vivo.";
RL Vet. Res. 50:80-80(2019).
RN [7]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=34133943; DOI=10.1016/j.cell.2021.05.017;
RA Li F., Jiang H., Shen X., Yang W., Guo C., Wang Z., Xiao M., Cui L.,
RA Luo W., Kim B.S., Chen Z., Huang A.J.W., Liu Q.;
RT "Sneezing reflex is mediated by a peptidergic pathway from nose to
RT brainstem.";
RL Cell 184:3762-3773(2021).
CC -!- FUNCTION: Stimulates smooth muscle contraction (By similarity). Induces
CC sighing by acting directly on the pre-Botzinger complex, a cluster of
CC several thousand neurons in the ventrolateral medulla responsible for
CC inspiration during respiratory activity (PubMed:26855425). Contributes
CC to the induction of sneezing following exposure to chemical irritants
CC or allergens which causes release of NMB by nasal sensory neurons and
CC activation of NMBR-expressing neurons in the sneeze-evoking region of
CC the brainstem (PubMed:34133943). These in turn activate neurons of the
CC caudal ventral respiratory group, giving rise to the sneeze reflex
CC (PubMed:34133943). Contributes to induction of acute itch, possibly
CC through activation of the NMBR receptor on dorsal root ganglion neurons
CC (PubMed:30734045). Increases expression of NMBR and steroidogenic
CC mediators STAR, CYP11A1 and HSD3B1 in Leydig cells, induces secretion
CC of testosterone by Leydig cells and also promotes Leydig cell
CC proliferation (By similarity). Plays a role in the innate immune
CC response to influenza A virus infection by enhancing interferon alpha
CC expression and reducing expression of IL6 (PubMed:31601264). Plays a
CC role in CSF1-induced proliferation of osteoclast precursors by
CC contributing to the positive regulation of the expression of the CSF1
CC receptor CSF1R (PubMed:28780306). {ECO:0000250|UniProtKB:P01297,
CC ECO:0000269|PubMed:26855425, ECO:0000269|PubMed:28780306,
CC ECO:0000269|PubMed:30734045, ECO:0000269|PubMed:31601264,
CC ECO:0000269|PubMed:34133943}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:34133943}. Cell
CC projection, neuron projection {ECO:0000269|PubMed:26855425}. Note=In
CC neurons of the retrotrapezoid nucleus//parafacial respiratory group,
CC expressed on neuron projections which project into the pre-Botzinger
CC complex. {ECO:0000269|PubMed:26855425}.
CC -!- TISSUE SPECIFICITY: In the hindbrain, expressed in the medulla
CC surrounding the lateral half of the facial nucleus (PubMed:26855425).
CC Also expressed in the olfactory bulb and hippocampus (PubMed:26855425).
CC Detected in a subset of neurons distributed throughout the
CC retrotrapezoid nucleus/parafacial respiratory group (RTN/pFRG)
CC (PubMed:26855425). Within the RTN/pFRG, expressed in neuronal
CC subpopulations distinct from those expressing Grp (PubMed:26855425).
CC Expressed in lung (PubMed:31601264). {ECO:0000269|PubMed:26855425,
CC ECO:0000269|PubMed:31601264}.
CC -!- DEVELOPMENTAL STAGE: During osteoclast development, expression
CC increases as the cells differentiate with high expression levels in
CC mature osteoclasts (at protein level). {ECO:0000269|PubMed:28780306}.
CC -!- INDUCTION: Up-regulated in lung tissue in response to infection with
CC influenza A virus. {ECO:0000269|PubMed:31601264}.
CC -!- DISRUPTION PHENOTYPE: Significant reduction in the sneezing response to
CC capsaicin at both low and high doses but does not affect pain-related
CC nose wiping behavior or apnea induced by capsaicin (PubMed:34133943).
CC Abolishes the sneezing response to histamine, seratonin and the
CC neuropeptide Npff (PubMed:34133943). Abolishes sneezing response to
CC mast cell-dependent allergy (PubMed:34133943).
CC {ECO:0000269|PubMed:34133943}.
CC -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC {ECO:0000305}.
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DR EMBL; AK014077; BAB29144.1; -; mRNA.
DR EMBL; AK011929; BAB27922.1; -; mRNA.
DR EMBL; BC028490; AAH28490.1; -; mRNA.
DR CCDS; CCDS21400.1; -.
DR RefSeq; NP_080799.1; NM_026523.4.
DR AlphaFoldDB; Q9CR53; -.
DR STRING; 10090.ENSMUSP00000026817; -.
DR PhosphoSitePlus; Q9CR53; -.
DR PaxDb; Q9CR53; -.
DR PRIDE; Q9CR53; -.
DR ProteomicsDB; 293688; -.
DR Antibodypedia; 43613; 159 antibodies from 27 providers.
DR DNASU; 68039; -.
DR Ensembl; ENSMUST00000026817; ENSMUSP00000026817; ENSMUSG00000025723.
DR GeneID; 68039; -.
DR KEGG; mmu:68039; -.
DR UCSC; uc009ibm.3; mouse.
DR CTD; 4828; -.
DR MGI; MGI:1915289; Nmb.
DR VEuPathDB; HostDB:ENSMUSG00000025723; -.
DR eggNOG; ENOG502S66V; Eukaryota.
DR GeneTree; ENSGT00940000154470; -.
DR HOGENOM; CLU_136527_0_0_1; -.
DR InParanoid; Q9CR53; -.
DR PhylomeDB; Q9CR53; -.
DR TreeFam; TF336860; -.
DR Reactome; R-MMU-375276; Peptide ligand-binding receptors.
DR Reactome; R-MMU-416476; G alpha (q) signalling events.
DR BioGRID-ORCS; 68039; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q9CR53; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9CR53; protein.
DR Bgee; ENSMUSG00000025723; Expressed in lumbar dorsal root ganglion and 134 other tissues.
DR ExpressionAtlas; Q9CR53; baseline and differential.
DR Genevisible; Q9CR53; MM.
DR GO; GO:0005615; C:extracellular space; IDA:UniProtKB.
DR GO; GO:0043005; C:neuron projection; IDA:UniProtKB.
DR GO; GO:0031710; F:neuromedin B receptor binding; ISO:MGI.
DR GO; GO:0005184; F:neuropeptide hormone activity; IBA:GO_Central.
DR GO; GO:0140374; P:antiviral innate immune response; IDA:UniProtKB.
DR GO; GO:0050482; P:arachidonic acid secretion; ISO:MGI.
DR GO; GO:0042593; P:glucose homeostasis; ISO:MGI.
DR GO; GO:0160024; P:Leydig cell proliferation; ISS:UniProtKB.
DR GO; GO:0046888; P:negative regulation of hormone secretion; ISO:MGI.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; IMP:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; ISO:MGI.
DR GO; GO:0046887; P:positive regulation of hormone secretion; ISO:MGI.
DR GO; GO:0032727; P:positive regulation of interferon-alpha production; IDA:UniProtKB.
DR GO; GO:0090290; P:positive regulation of osteoclast proliferation; IMP:UniProtKB.
DR GO; GO:1903942; P:positive regulation of respiratory gaseous exchange; IDA:UniProtKB.
DR GO; GO:2000845; P:positive regulation of testosterone secretion; ISS:UniProtKB.
DR GO; GO:0160025; P:sensory perception of itch; IDA:UniProtKB.
DR GO; GO:0160023; P:sneeze reflex; IMP:UniProtKB.
DR InterPro; IPR000874; Bombesin.
DR PANTHER; PTHR16866; PTHR16866; 1.
DR Pfam; PF02044; Bombesin; 1.
DR PROSITE; PS00257; BOMBESIN; 1.
PE 1: Evidence at protein level;
KW Amidation; Cell projection; Cleavage on pair of basic residues; Immunity;
KW Innate immunity; Reference proteome; Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000250|UniProtKB:P01297"
FT PEPTIDE 25..56
FT /note="Neuromedin-B-32"
FT /evidence="ECO:0000250|UniProtKB:P01297"
FT /id="PRO_0000003020"
FT PEPTIDE 47..56
FT /note="Neuromedin-B"
FT /evidence="ECO:0000250|UniProtKB:P01297"
FT /id="PRO_0000003021"
FT PROPEP 60..121
FT /evidence="ECO:0000250|UniProtKB:P01297"
FT /id="PRO_0000003022"
FT MOD_RES 56
FT /note="Methionine amide"
FT /evidence="ECO:0000250|UniProtKB:P01297"
SQ SEQUENCE 121 AA; 13571 MW; 1EE94C963D3D9D31 CRC64;
MTRQAGSSWL LRGLLLFALF ASGVAPFNWD LPEPRSRASK IRVHPRGNLW ATGHFMGKKS
LEPPSLSLVG TAPPNTPRDQ RLQLSHDLLR ILLRKKALGM NFSGPAPPIQ YRRLLEPLLQ
K
