NMB_PIG
ID NMB_PIG Reviewed; 121 AA.
AC P01297; A0A4X1UK00;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 77.
DE RecName: Full=Neuromedin-B;
DE Contains:
DE RecName: Full=Neuromedin-B-32;
DE Contains:
DE RecName: Full=Neuromedin-B-30;
DE Contains:
DE RecName: Full=Neuromedin-B;
DE Flags: Precursor;
GN Name=NMB;
OS Sus scrofa (Pig).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Suina; Suidae; Sus.
OX NCBI_TaxID=9823;
RN [1] {ECO:0000312|Proteomes:UP000314985}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Hannum G.I., Koren S., Schroeder S.G., Chin S.C., Nonneman D.J.,
RA Becker S.A., Rosen B.D., Bickhart D.M., Putnam N.H., Green R.E.,
RA Tuggle C.K., Liu H., Rohrer G.A., Warr A., Hall R., Kim K., Hume D.A.,
RA Talbot R., Chow W., Howe K., Schwartz A.S., Watson M., Archibald A.L.,
RA Phillippy A.M., Smith T.P.L.;
RT "USMARCv1.0.";
RL Submitted (AUG-2017) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP PROTEIN SEQUENCE OF 25-56.
RX PubMed=4026853; DOI=10.1016/0006-291x(85)90471-1;
RA Minamino N., Sudoh T., Kangawa K., Matsuo H.;
RT "Neuromedin B-32 and B-30: two 'big' neuromedin B identified in porcine
RT brain and spinal cord.";
RL Biochem. Biophys. Res. Commun. 130:685-691(1985).
RN [3]
RP PROTEIN SEQUENCE OF 25-56.
RA Minamino N., Kangawa K., Matsuo H.;
RT "Neuromedin B and neuromedin C: two mammalian bombesin-like peptides
RT identified in pig spinal cord and brain.";
RL Regul. Pept. 19:127-127(1987).
RN [4]
RP PROTEIN SEQUENCE OF 47-56, FUNCTION, AND AMIDATION AT MET-56.
RX PubMed=6882442; DOI=10.1016/0006-291x(83)90814-8;
RA Minamino N., Kangawa K., Matsuo H.;
RT "Neuromedin B: a novel bombesin-like peptide identified in porcine spinal
RT cord.";
RL Biochem. Biophys. Res. Commun. 114:541-548(1983).
RN [5]
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=27010315; DOI=10.1371/journal.pone.0151871;
RA Ma Z., Su J., Guo T., Jin M., Li X., Lei Z., Hou Y., Li X., Jia C.,
RA Zhang Z., Ahmed E.;
RT "Neuromedin B and Its Receptor: Gene Cloning, Tissue Distribution and
RT Expression Levels of the Reproductive Axis in Pigs.";
RL PLoS ONE 11:e0151871-e0151871(2016).
RN [6]
RP FUNCTION.
RX PubMed=29632025; DOI=10.1530/jme-17-0242;
RA Ma Z., Zhang Y., Su J., Yang S., Qiao W., Li X., Lei Z., Cheng L., An N.,
RA Wang W., Feng Y., Zhang J.;
RT "Effects of neuromedin B on steroidogenesis, cell proliferation and
RT apoptosis in porcine Leydig cells.";
RL J. Mol. Endocrinol. 61:13-23(2018).
CC -!- FUNCTION: Stimulates smooth muscle contraction (PubMed:6882442).
CC Induces sighing by acting directly on the pre-Botzinger complex, a
CC cluster of several thousand neurons in the ventrolateral medulla
CC responsible for inspiration during respiratory activity (By
CC similarity). Contributes to the induction of sneezing following
CC exposure to chemical irritants or allergens which causes release of NMB
CC by nasal sensory neurons and activation of NMBR-expressing neurons in
CC the sneeze-evoking region of the brainstem (By similarity). These in
CC turn activate neurons of the caudal ventral respiratory group, giving
CC rise to the sneezing response (By similarity). Contributes to induction
CC of acute itch, possibly through activation of the NMBR receptor on
CC dorsal root ganglion neurons (By similarity). Increases expression of
CC NMBR and steroidogenic mediators STAR, CYP11A1 and HSD3B1 in Leydig
CC cells, induces secretion of testosterone by Leydig cells and also
CC promotes Leydig cell proliferation (PubMed:29632025). Plays a role in
CC the innate immune response to influenza A virus infection by enhancing
CC interferon alpha expression and reducing expression of IL6 (By
CC similarity). Plays a role in CSF1-induced proliferation of osteoclast
CC precursors by contributing to positive regulation of the expression of
CC the CSF1 receptor CSF1R (By similarity). {ECO:0000250|UniProtKB:Q9CR53,
CC ECO:0000269|PubMed:29632025, ECO:0000269|PubMed:6882442}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q9CR53}. Cell
CC projection, neuron projection {ECO:0000250|UniProtKB:Q9CR53}. Note=In
CC neurons of the retrotrapezoid nucleus//parafacial respiratory group,
CC expressed on neuron projections which project into the pre-Botzinger
CC complex. {ECO:0000250|UniProtKB:Q9CR53}.
CC -!- TISSUE SPECIFICITY: Higher expression in the central nervous system
CC (CNS) than in peripheral tissues. Highest levels are found in the
CC olfactory bulb. Relatively high levels in the CNS (including the
CC cerebral cortex, cerebellum, spinal cord, medulla oblongata, midbrain,
CC hypothalamus, hippocampus, and hypophysis) and in peripheral tissues
CC such as the pancreas, adrenal gland, testis, ovary and cecum. Moderate
CC levels are found in the rectum, heart and pons with low expression
CC levels detected in the bone marrow and duodenum. Other tissues show no
CC or low levels of expression. {ECO:0000269|PubMed:27010315}.
CC -!- DEVELOPMENTAL STAGE: During the sow estrus cycle, highest levels are
CC found in the hypothalamus during proestrus, decrease during estrus and
CC metestrus and increase again during diestrus. In the pituitary gland,
CC levels decrease from proestrus to estrus, increase at metestrus and
CC decrease again at diestrus. In the ovary, expression peaks at
CC proestrus, decreases slightly at estrus, decreases significantly at
CC metestrus and increases at diestrus. During boar postnatal development,
CC expression peaks in the hypothalamus at day 60 and decreases
CC thereafter. In the pituitary gland, expression peaks at day 30 and
CC decreases thereafter. In the testis, expression peaks at day 3 with
CC lowest levels at day 60 and expression then gradually increases from
CC day 60 to day 120. {ECO:0000269|PubMed:27010315}.
CC -!- SIMILARITY: Belongs to the bombesin/neuromedin-B/ranatensin family.
CC {ECO:0000305}.
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DR PIR; B60301; BSPGNB.
DR RefSeq; NP_001116617.1; NM_001123145.1.
DR AlphaFoldDB; P01297; -.
DR SMR; P01297; -.
DR STRING; 9823.ENSSSCP00000027260; -.
DR PaxDb; P01297; -.
DR Ensembl; ENSSSCT00025088850; ENSSSCP00025038837; ENSSSCG00025064766.
DR Ensembl; ENSSSCT00065015100; ENSSSCP00065006159; ENSSSCG00065011355.
DR Ensembl; ENSSSCT00070034629; ENSSSCP00070028926; ENSSSCG00070017548.
DR GeneID; 100141313; -.
DR KEGG; ssc:100141313; -.
DR CTD; 4828; -.
DR eggNOG; ENOG502S66V; Eukaryota.
DR HOGENOM; CLU_208788_0_0_1; -.
DR InParanoid; P01297; -.
DR OMA; NTAEMIP; -.
DR Proteomes; UP000008227; Unplaced.
DR Proteomes; UP000314985; Chromosome 7.
DR GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR GO; GO:0043005; C:neuron projection; ISS:UniProtKB.
DR GO; GO:0031710; F:neuromedin B receptor binding; IBA:GO_Central.
DR GO; GO:0005184; F:neuropeptide hormone activity; IBA:GO_Central.
DR GO; GO:0140374; P:antiviral innate immune response; ISS:UniProtKB.
DR GO; GO:0160024; P:Leydig cell proliferation; IDA:UniProtKB.
DR GO; GO:0032715; P:negative regulation of interleukin-6 production; ISS:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IEA:InterPro.
DR GO; GO:0046887; P:positive regulation of hormone secretion; IBA:GO_Central.
DR GO; GO:0032727; P:positive regulation of interferon-alpha production; ISS:UniProtKB.
DR GO; GO:0090290; P:positive regulation of osteoclast proliferation; ISS:UniProtKB.
DR GO; GO:1903942; P:positive regulation of respiratory gaseous exchange; ISS:UniProtKB.
DR GO; GO:2000845; P:positive regulation of testosterone secretion; IDA:UniProtKB.
DR GO; GO:0160025; P:sensory perception of itch; ISS:UniProtKB.
DR GO; GO:0160023; P:sneeze reflex; ISS:UniProtKB.
DR InterPro; IPR000874; Bombesin.
DR PANTHER; PTHR16866; PTHR16866; 1.
DR Pfam; PF02044; Bombesin; 1.
DR PROSITE; PS00257; BOMBESIN; 1.
PE 1: Evidence at protein level;
KW Amidation; Cell projection; Cleavage on pair of basic residues;
KW Direct protein sequencing; Immunity; Innate immunity; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000269|PubMed:4026853, ECO:0000269|Ref.3"
FT PEPTIDE 25..56
FT /note="Neuromedin-B-32"
FT /evidence="ECO:0000269|PubMed:4026853, ECO:0000269|Ref.3"
FT /id="PRO_0000045912"
FT PEPTIDE 27..56
FT /note="Neuromedin-B-30"
FT /evidence="ECO:0000269|PubMed:4026853"
FT /id="PRO_0000262471"
FT PEPTIDE 47..56
FT /note="Neuromedin-B"
FT /evidence="ECO:0000269|PubMed:6882442"
FT /id="PRO_0000003023"
FT PROPEP 60..121
FT /evidence="ECO:0000269|PubMed:4026853,
FT ECO:0000269|PubMed:6882442, ECO:0000269|Ref.3"
FT /id="PRO_0000455630"
FT MOD_RES 56
FT /note="Methionine amide"
FT /evidence="ECO:0000269|PubMed:6882442"
SQ SEQUENCE 121 AA; 13381 MW; 002616317CE14EC4 CRC64;
MTLRARGARL LGGLLFFTLL AAGAAPLSWD LPEPRSRAGK IRVHPRGNLW ATGHFMGKKS
LEPPNPSLLG TTHHISLRDQ RLQLSHDLLR ILLQKKALGL SLSGPASHTP YRRLLVQTLE
K
