NMD3_MOUSE
ID NMD3_MOUSE Reviewed; 503 AA.
AC Q99L48; Q3UPR1; Q8BM61;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=60S ribosomal export protein NMD3;
GN Name=Nmd3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J, and NOD;
RC TISSUE=Embryo, Mammary gland, Oviduct, and Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-468 AND THR-470, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney, Liver, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Acts as an adapter for the XPO1/CRM1-mediated export of the
CC 60S ribosomal subunit. {ECO:0000250|UniProtKB:Q96D46}.
CC -!- SUBUNIT: Found in a 60S ribosomal subunit export complex with RAN and
CC XPO1. Interacts with XPO1. Associates with pre-60S ribosomal particles.
CC {ECO:0000250|UniProtKB:Q96D46}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q96D46}. Nucleus
CC {ECO:0000250|UniProtKB:Q96D46}. Note=Shuttles between the
CC nucleus/nucleolus and the cytoplasm in a XPO1/CRM1-dependent manner.
CC {ECO:0000250|UniProtKB:Q96D46}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q99L48-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q99L48-2; Sequence=VSP_032020, VSP_032021;
CC -!- SIMILARITY: Belongs to the NMD3 family. {ECO:0000305}.
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DR EMBL; AK034772; BAC28829.1; -; mRNA.
DR EMBL; AK088667; BAC40491.1; -; mRNA.
DR EMBL; AK143289; BAE25334.1; -; mRNA.
DR EMBL; AK145248; BAE26326.1; -; mRNA.
DR EMBL; AK166283; BAE38680.1; -; mRNA.
DR EMBL; BC003842; AAH03842.1; -; mRNA.
DR CCDS; CCDS17407.1; -. [Q99L48-1]
DR RefSeq; NP_598548.1; NM_133787.2. [Q99L48-1]
DR AlphaFoldDB; Q99L48; -.
DR SMR; Q99L48; -.
DR BioGRID; 220607; 1.
DR STRING; 10090.ENSMUSP00000029358; -.
DR iPTMnet; Q99L48; -.
DR PhosphoSitePlus; Q99L48; -.
DR SwissPalm; Q99L48; -.
DR EPD; Q99L48; -.
DR jPOST; Q99L48; -.
DR MaxQB; Q99L48; -.
DR PaxDb; Q99L48; -.
DR PeptideAtlas; Q99L48; -.
DR PRIDE; Q99L48; -.
DR ProteomicsDB; 293862; -. [Q99L48-1]
DR ProteomicsDB; 293863; -. [Q99L48-2]
DR Antibodypedia; 33677; 131 antibodies from 27 providers.
DR DNASU; 97112; -.
DR Ensembl; ENSMUST00000029358; ENSMUSP00000029358; ENSMUSG00000027787. [Q99L48-1]
DR GeneID; 97112; -.
DR KEGG; mmu:97112; -.
DR UCSC; uc008pmk.1; mouse. [Q99L48-2]
DR UCSC; uc008pml.1; mouse. [Q99L48-1]
DR CTD; 51068; -.
DR MGI; MGI:2140103; Nmd3.
DR VEuPathDB; HostDB:ENSMUSG00000027787; -.
DR eggNOG; KOG2613; Eukaryota.
DR GeneTree; ENSGT00390000005104; -.
DR HOGENOM; CLU_027444_2_0_1; -.
DR InParanoid; Q99L48; -.
DR OMA; ILVKKHY; -.
DR OrthoDB; 734666at2759; -.
DR PhylomeDB; Q99L48; -.
DR TreeFam; TF105744; -.
DR BioGRID-ORCS; 97112; 25 hits in 72 CRISPR screens.
DR PRO; PR:Q99L48; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q99L48; protein.
DR Bgee; ENSMUSG00000027787; Expressed in ectoplacental cone and 270 other tissues.
DR ExpressionAtlas; Q99L48; baseline and differential.
DR Genevisible; Q99L48; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISO:MGI.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; ISO:MGI.
DR GO; GO:0043023; F:ribosomal large subunit binding; ISO:MGI.
DR GO; GO:0032092; P:positive regulation of protein binding; ISO:MGI.
DR GO; GO:1904751; P:positive regulation of protein localization to nucleolus; ISO:MGI.
DR GO; GO:1902680; P:positive regulation of RNA biosynthetic process; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; ISO:MGI.
DR InterPro; IPR039768; Nmd3.
DR InterPro; IPR007064; Nmd3_N.
DR PANTHER; PTHR12746; PTHR12746; 1.
DR Pfam; PF04981; NMD3; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; Cytoplasm; Nucleus; Phosphoprotein;
KW Protein transport; Reference proteome; Transport.
FT CHAIN 1..503
FT /note="60S ribosomal export protein NMD3"
FT /id="PRO_0000323562"
FT REGION 425..503
FT /note="Necessary for the nuclear export of the 60S
FT ribosomal subunit"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOTIF 405..422
FT /note="Nuclear and nucleolar localization signal"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOTIF 480..489
FT /note="Nuclear export signal"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOD_RES 433
FT /note="Phosphothreonine"
FT /evidence="ECO:0000250|UniProtKB:Q96D46"
FT MOD_RES 468
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 470
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 193..200
FT /note="DGLDFYYS -> GEFKFKRI (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032020"
FT VAR_SEQ 201..503
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032021"
SQ SEQUENCE 503 AA; 57611 MW; 44C73CC2E5D473EE CRC64;
MEYMAESIEH SPGHILCCEC GVPISPNPAN ICVACLRSKV DISQGVPKQV SISFCKQCQR
YFQPPASWVQ CALESRELLA LCLKKIKAPL SKVRLVDAGF VWTEPHSKRL KVKLTIQKEV
MNGAILQQVF VVDYVVQSQM CGDCHRVEAK DFWKAVIQVR QKTLHKKTFY YLEQLILKYG
MHQNTLRIKE IHDGLDFYYS SKQHAQKMVE FLQGIVPCRY KASQRLISQD IHSNTYNYKS
TFSVEIVPIC KDNVVCLSPK LAQSLGNMSQ ICICIRVTSA IHLIDPNTLQ VADIDGNTFW
SHPFNSLCHP KQLEEFIVIE CSTVRDLKRS AGAGVISKKH TLGEVWIQKT SEMNTDKQYF
CRTHLGHLLN PGDLVLGFDL ANCNLNDEYV NKMNSDRVPD VVLIKKSYDR TKRQRRRNWK
LKELARDREN MDTDDERQYQ DFLEDLEEDE AIRKHVNIYR DATIPVESDT DDEGAPRISL
AEMLEDLHIS QDATGEEGAS MMS
