NMD3_YEAST
ID NMD3_YEAST Reviewed; 518 AA.
AC P38861; D3DLB9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 05-OCT-2010, sequence version 3.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=60S ribosomal export protein NMD3;
DE AltName: Full=Nonsense-mediated mRNA decay protein 3;
GN Name=NMD3; OrderedLocusNames=YHR170W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA He F., Jacobson A.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8091229; DOI=10.1126/science.8091229;
RA Johnston M., Andrews S., Brinkman R., Cooper J., Ding H., Dover J., Du Z.,
RA Favello A., Fulton L., Gattung S., Geisel C., Kirsten J., Kucaba T.,
RA Hillier L.W., Jier M., Johnston L., Langston Y., Latreille P., Louis E.J.,
RA Macri C., Mardis E., Menezes S., Mouser L., Nhan M., Rifkin L., Riles L.,
RA St Peter H., Trevaskis E., Vaughan K., Vignati D., Wilcox L., Wohldman P.,
RA Waterston R., Wilson R., Vaudin M.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome
RT VIII.";
RL Science 265:2077-2082(1994).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, ASSOCIATION WITH THE 60S RIBOSOMAL SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=11086007; DOI=10.1083/jcb.151.5.1057;
RA Ho J.H., Kallstrom G., Johnson A.W.;
RT "Nmd3p is a Crm1p-dependent adapter protein for nuclear export of the large
RT ribosomal subunit.";
RL J. Cell Biol. 151:1057-1066(2000).
CC -!- FUNCTION: Acts as an adapter for the XPO1/CRM1-mediated export of the
CC 60S ribosomal subunit. Unlikely to play a significant role in nonsense-
CC mediated mRNA decay (NMD). {ECO:0000269|PubMed:11086007}.
CC -!- SUBUNIT: Associates with the 60S ribosomal subunit.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:11086007}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:11086007}. Note=Shuttles between the
CC nucleus and the cytoplasm in a XPO1/CRM1-dependent manner.
CC -!- MISCELLANEOUS: Present with 11900 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the NMD3 family. {ECO:0000305}.
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DR EMBL; U31376; AAA74491.1; -; Genomic_DNA.
DR EMBL; U00027; AAB68015.1; -; Genomic_DNA.
DR EMBL; BK006934; DAA06863.1; -; Genomic_DNA.
DR PIR; S48909; S48909.
DR RefSeq; NP_012040.1; NM_001179301.1.
DR PDB; 5H4P; EM; 3.07 A; w=155-402.
DR PDB; 5T62; EM; 3.30 A; V=1-518.
DR PDB; 5T6R; EM; 4.50 A; V=2-518.
DR PDB; 6N8K; EM; 3.60 A; v=1-518.
DR PDB; 6N8L; EM; 3.60 A; v=1-518.
DR PDB; 6N8M; EM; 3.50 A; V=1-518.
DR PDB; 6N8N; EM; 3.80 A; V=1-518.
DR PDB; 6N8O; EM; 3.50 A; V=1-518.
DR PDB; 6QIK; EM; 3.10 A; w=1-518.
DR PDB; 6QTZ; EM; 3.50 A; w=1-518.
DR PDB; 6RI5; EM; 3.30 A; w=1-518.
DR PDB; 6RZZ; EM; 3.20 A; w=1-518.
DR PDB; 6S05; EM; 3.90 A; w=1-518.
DR PDBsum; 5H4P; -.
DR PDBsum; 5T62; -.
DR PDBsum; 5T6R; -.
DR PDBsum; 6N8K; -.
DR PDBsum; 6N8L; -.
DR PDBsum; 6N8M; -.
DR PDBsum; 6N8N; -.
DR PDBsum; 6N8O; -.
DR PDBsum; 6QIK; -.
DR PDBsum; 6QTZ; -.
DR PDBsum; 6RI5; -.
DR PDBsum; 6RZZ; -.
DR PDBsum; 6S05; -.
DR AlphaFoldDB; P38861; -.
DR SMR; P38861; -.
DR BioGRID; 36604; 183.
DR DIP; DIP-1827N; -.
DR IntAct; P38861; 33.
DR MINT; P38861; -.
DR STRING; 4932.YHR170W; -.
DR iPTMnet; P38861; -.
DR MaxQB; P38861; -.
DR PaxDb; P38861; -.
DR PRIDE; P38861; -.
DR EnsemblFungi; YHR170W_mRNA; YHR170W; YHR170W.
DR GeneID; 856575; -.
DR KEGG; sce:YHR170W; -.
DR SGD; S000001213; NMD3.
DR VEuPathDB; FungiDB:YHR170W; -.
DR eggNOG; KOG2613; Eukaryota.
DR GeneTree; ENSGT00390000005104; -.
DR HOGENOM; CLU_027444_2_0_1; -.
DR InParanoid; P38861; -.
DR OMA; ILVKKHY; -.
DR BioCyc; YEAST:G3O-31204-MON; -.
DR PRO; PR:P38861; -.
DR Proteomes; UP000002311; Chromosome VIII.
DR RNAct; P38861; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0070180; F:large ribosomal subunit rRNA binding; IDA:SGD.
DR GO; GO:0043023; F:ribosomal large subunit binding; IDA:SGD.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0000055; P:ribosomal large subunit export from nucleus; IMP:SGD.
DR InterPro; IPR039768; Nmd3.
DR InterPro; IPR007064; Nmd3_N.
DR PANTHER; PTHR12746; PTHR12746; 1.
DR Pfam; PF04981; NMD3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; Nucleus; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..518
FT /note="60S ribosomal export protein NMD3"
FT /id="PRO_0000096874"
FT REGION 466..491
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 399..415
FT /note="Nuclear localization signal"
FT MOTIF 493..502
FT /note="Nuclear export signal"
FT COMPBIAS 477..491
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 94
FT /note="K -> F (in Ref. 1; AAA74491)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 518 AA; 59095 MW; 86405DDE49B3DCB4 CRC64;
MEFTPIDPHQ HQNAATLLCC NCGTPIDGST GLVMCYDCIK LTVDITQGIP REANISFCRN
CERFLQPPGQ WIRAELESRE LLAICLRRLK GLTKVRLVDA SFIWTEPHSR RIRIKLTVQG
EAMTNTIIQQ TFEVEYIVIA MQCPDCARSY TTNTWRATVQ IRQKVPHKRT FLFLEQLILK
HNAHVDTISI SEAKDGLDFF YAQKNHAVKM IDFLNAVVPI KHKKSEELIS QDTHTGASTY
KFSYSVEIVP ICKDDLVVLP KKLAKSMGNI SQFVLCSKIS NTVQFMDPTT LQTADLSPSV
YWRAPFNALA DVTQLVEFIV LDVDSTGISR GNRVLADITV ARTSDLGVND QVYYVRSHLG
GICHAGDSVM GYFIANSNYN SDLFDGLNID YVPDVVLVKK LYQRKSKKSR HWKLKRMAKE
HKDIDASLDY NSRAQKQEME RAEKDYELFL QELEEDAELR QSVNLYKNRE ANVPPEEHEM
DEDEDEDAPQ INIDELLDEL DEMTLEDGVE NTPVESQQ
