ID   NMD4_YEAST              Reviewed;         218 AA.
AC   Q12129; D6VZ01;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 129.
DE   RecName: Full=Nonsense-mediated decay protein 4;
GN   Name=NMD4; OrderedLocusNames=YLR363C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169871;
RA   Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA   Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA   Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA   Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA   Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA   Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA   Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA   Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA   Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA   Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA   Zollner A., Hani J., Hoheisel J.D.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL   Nature 387:87-90(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   FUNCTION.
RX   PubMed=7883168; DOI=10.1101/gad.9.4.437;
RA   He F., Jacobson A.;
RT   "Identification of a novel component of the nonsense-mediated mRNA decay
RT   pathway by use of an interacting protein screen.";
RL   Genes Dev. 9:437-454(1995).
RN   [5]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [6]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Involved in nonsense-mediated decay of mRNAs containing
CC       premature stop codons. {ECO:0000269|PubMed:7883168}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC   -!- MISCELLANEOUS: Present with 6090 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; U31374; AAA74489.1; -; Genomic_DNA.
DR   EMBL; U19103; AAB67575.1; -; Genomic_DNA.
DR   EMBL; AY558223; AAS56549.1; -; Genomic_DNA.
DR   EMBL; BK006945; DAA09667.1; -; Genomic_DNA.
DR   PIR; S51381; S51381.
DR   RefSeq; NP_013467.3; NM_001182252.3.
DR   AlphaFoldDB; Q12129; -.
DR   SMR; Q12129; -.
DR   BioGRID; 31624; 77.
DR   DIP; DIP-2439N; -.
DR   IntAct; Q12129; 17.
DR   STRING; 4932.YLR363C; -.
DR   iPTMnet; Q12129; -.
DR   MaxQB; Q12129; -.
DR   PaxDb; Q12129; -.
DR   PRIDE; Q12129; -.
DR   TopDownProteomics; Q12129; -.
DR   EnsemblFungi; YLR363C_mRNA; YLR363C; YLR363C.
DR   GeneID; 851077; -.
DR   KEGG; sce:YLR363C; -.
DR   SGD; S000004355; NMD4.
DR   VEuPathDB; FungiDB:YLR363C; -.
DR   eggNOG; ENOG502RYBU; Eukaryota.
DR   HOGENOM; CLU_1273151_0_0_1; -.
DR   InParanoid; Q12129; -.
DR   OMA; PTYTLKE; -.
DR   BioCyc; YEAST:G3O-32434-MON; -.
DR   PRO; PR:Q12129; -.
DR   Proteomes; UP000002311; Chromosome XII.
DR   RNAct; Q12129; protein.
DR   GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR   GO; GO:0005634; C:nucleus; HDA:SGD.
DR   GO; GO:0000184; P:nuclear-transcribed mRNA catabolic process, nonsense-mediated decay; IPI:SGD.
DR   InterPro; IPR002716; PIN_dom.
DR   SMART; SM00670; PINc; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Nonsense-mediated mRNA decay; Reference proteome.
FT   CHAIN           1..218
FT                   /note="Nonsense-mediated decay protein 4"
FT                   /id="PRO_0000096879"
SQ   SEQUENCE   218 AA;  25286 MW;  ABB44FD0C5CBB4C7 CRC64;
     MTQYNFIIDA SAFEKGLGNI KRWCSDCTEA VTLNFYIPTF TLNELDFLQQ RRKSFAARES
     LKFIDRLDDS KFANLKVFIE FPEVLDIILW SDVMEHNDSS GKINIAKLPK RLKNLLKSCI
     YKCYLEGNEG LHWFLISEDP QIREMAMQCN IPSCSIVDVD SILSKDMNDK SFRESEKFNN
     MMLKNGTKEE SENGREIIRT NFNKTVYASR GTGELWSP