NMD5_YEAST
ID NMD5_YEAST Reviewed; 1048 AA.
AC P46970; D6VWV0;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 2.
DT 03-AUG-2022, entry version 170.
DE RecName: Full=Nonsense-mediated mRNA decay protein 5;
DE AltName: Full=Karyopherin-119;
GN Name=NMD5; Synonyms=KAP119; OrderedLocusNames=YJR132W; ORFNames=J2112;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA He F., Jacobson A.;
RL Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL EMBO J. 15:2031-2049(1996).
RN [3]
RP GENOME REANNOTATION, AND SEQUENCE REVISION TO 258.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, AND SUBUNIT.
RX PubMed=9852143; DOI=10.1083/jcb.143.6.1447;
RA Albertini M., Pemberton L.F., Rosenblum J.S., Blobel G.;
RT "A novel nuclear import pathway for the transcription factor TFIIS.";
RL J. Cell Biol. 143:1447-1455(1998).
RN [5]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-977, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Active in protein import into the nucleus. Its major import
CC substrate is transcription elongation factor TFIIS.
CC {ECO:0000269|PubMed:9852143}.
CC -!- SUBUNIT: GTP-bound Ran dissociates the isolated NMD5/TFIIS complex.
CC {ECO:0000269|PubMed:9852143}.
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
CC -!- MISCELLANEOUS: Present with 13500 molecules/cell in log phase SD
CC medium. {ECO:0000269|PubMed:14562106}.
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DR EMBL; U31375; AAA74490.1; -; Genomic_DNA.
DR EMBL; Z49632; CAA89663.1; -; Genomic_DNA.
DR EMBL; BK006943; DAA08916.2; -; Genomic_DNA.
DR PIR; S57155; S57155.
DR RefSeq; NP_012666.2; NM_001181790.2.
DR AlphaFoldDB; P46970; -.
DR BioGRID; 33887; 83.
DR DIP; DIP-2326N; -.
DR IntAct; P46970; 41.
DR MINT; P46970; -.
DR STRING; 4932.YJR132W; -.
DR iPTMnet; P46970; -.
DR MaxQB; P46970; -.
DR PaxDb; P46970; -.
DR PRIDE; P46970; -.
DR EnsemblFungi; YJR132W_mRNA; YJR132W; YJR132W.
DR GeneID; 853596; -.
DR KEGG; sce:YJR132W; -.
DR SGD; S000003893; NMD5.
DR VEuPathDB; FungiDB:YJR132W; -.
DR eggNOG; KOG1991; Eukaryota.
DR HOGENOM; CLU_004196_0_0_1; -.
DR InParanoid; P46970; -.
DR OMA; NHWWKCK; -.
DR BioCyc; YEAST:G3O-31750-MON; -.
DR PRO; PR:P46970; -.
DR Proteomes; UP000002311; Chromosome X.
DR RNAct; P46970; protein.
DR GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005635; C:nuclear envelope; IBA:GO_Central.
DR GO; GO:0034399; C:nuclear periphery; HDA:SGD.
DR GO; GO:0005634; C:nucleus; IDA:SGD.
DR GO; GO:0061608; F:nuclear import signal receptor activity; IDA:SGD.
DR GO; GO:0031267; F:small GTPase binding; IEA:InterPro.
DR GO; GO:0006606; P:protein import into nucleus; IMP:SGD.
DR Gene3D; 1.25.10.10; -; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR001494; Importin-beta_N.
DR Pfam; PF03810; IBN_N; 1.
DR SMART; SM00913; IBN_N; 1.
DR SUPFAM; SSF48371; SSF48371; 1.
DR PROSITE; PS50166; IMPORTIN_B_NT; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..1048
FT /note="Nonsense-mediated mRNA decay protein 5"
FT /id="PRO_0000096880"
FT DOMAIN 24..104
FT /note="Importin N-terminal"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00115"
FT MOD_RES 977
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956,
FT ECO:0007744|PubMed:19779198"
FT CONFLICT 258
FT /note="A -> S (in Ref. 2; CAA89663)"
FT /evidence="ECO:0000305"
FT CONFLICT 517
FT /note="V -> I (in Ref. 1; AAA74490)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1048 AA; 119967 MW; 90312C1AAE515949 CRC64;
MDITELLQCF ACTLDHNAAV RTNAETHLKN ASKVPGFLGA CLDIIAADEV PENIKLSASL
YFKNKITYGW SAGARQGSNE LLDSHVDPDE KPVVKDMLIK TMVSVSKTSP RCIRVLKSAL
TVIISEDYPS KKWGNLLPNS LELLANEDIT VTYVGLLCLA EIFRTYRWKN NDERQDLEEL
ILNYFPALLN YGANVLFQDG KYMNNEQIGE LVKLIIKIYK FVSYHDLPFT LQRSESFTPW
ACFFVSIIQQ PLPQEVLAIS DIEVRSKNPW VKCKKWALAN LYRLFQRYAS TSLTRKFQYD
EFKQMYCEEF LTQFLQVVFD QIEKWGTGQL WLSDECLYYI LNFVEQCVVQ KTTWKLVGPH
YNVILQHVIF PLLKPTAETL EAFDNDPQEY INRNMDFWDV GYSPDLAALA LLTTCVTKRG
KTTLQPTLEF MVSTLQSAVG DYNNIMLDNA LQIESCLRIF SSIIDRLITK DSPFASEMEK
FILTYVLPFF KSQYGFLQSR VCDICSKLGS MDFKDPVITS TIYEGVMNCL NNSSNSLPVE
LTAALALQTF ISDDQFNMKL SEHVVPTMQK LLSLSNDFES DVISGVMQDF VEQFAEQLQP
FGVELMNTLV QQFLKLAIDL HETSNLDPDS FTNVDSIPDE SDKQMAALGI LSTTISILLS
FENSPEILKN LEQSFYPAAE FILKNDIEDF YRECCEFVEN STFLLRDITP ISWKILELIG
ECNRKPDSMV SYYLSDFMLA LNNILIYGRN ELKKNEFYTK IIFEIYQKAV TAEDNSLDDL
RVVFDLSQEL VLALDDSLPQ QYRERLLADV VGSILTQKNE LKTNVVFSVT AFNVVISNMI
TEPLITLQYL KQQGCLEIFF QTWITDYIPN YKRCYDIKLS VLALLKIILK LESNDYSVLN
LENLVPQLGS IVTQLASRLP TALRQLANQR KEFSSSGFEE DTKWDENFLD VGDDDENDDE
GDLTEKYLEL IKNRADSLDF VDGYDAKETF DDLEEDPLTG SILDTVDVYK VFKESIANLQ
HVDSNRYQGI LRHLTPADQE LFMGIMNA
