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NMRA_EMENI
ID   NMRA_EMENI              Reviewed;         352 AA.
AC   Q5AU62; C8V6Y1; O59919;
DT   20-APR-2010, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Nitrogen metabolite repression protein nmrA;
DE   AltName: Full=Negative-acting nitrogen regulatory protein nmrA;
DE   AltName: Full=Nitrogen metabolite regulation protein;
GN   Name=nmrA; ORFNames=AN8168;
OS   Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS   M139) (Aspergillus nidulans).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Nidulantes.
OX   NCBI_TaxID=227321;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DISRUPTION PHENOTYPE.
RX   PubMed=9537404; DOI=10.1128/jb.180.7.1973-1977.1998;
RA   Andrianopoulos A., Kourambas S., Sharp J.A., Davis M.A., Hynes M.J.;
RT   "Characterization of the Aspergillus nidulans nmrA gene involved in
RT   nitrogen metabolite repression.";
RL   J. Bacteriol. 180:1973-1977(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=16372000; DOI=10.1038/nature04341;
RA   Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA   Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA   Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA   Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA   Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA   Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA   Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA   Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA   Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT   "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT   fumigatus and A. oryzae.";
RL   Nature 438:1105-1115(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX   PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA   Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA   Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA   Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA   Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA   Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA   Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA   Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA   van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA   Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA   Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA   Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA   Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA   Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA   van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA   Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA   Oliver S.G., Turner G.;
RT   "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT   effort.";
RL   Fungal Genet. Biol. 46:S2-13(2009).
RN   [4]
RP   SUBUNIT.
RX   PubMed=11679757; DOI=10.1107/s090744490101410x;
RA   Nichols C.E., Cocklin S., Dodds A., Ren J., Lamb H., Hawkins A.R.,
RA   Stammers D.K.;
RT   "Expression, purification and crystallization of Aspergillus nidulans NmrA,
RT   a negative regulatory protein involved in nitrogen-metabolite repression.";
RL   Acta Crystallogr. D 57:1722-1725(2001).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY NITROGEN.
RX   PubMed=17854403; DOI=10.1111/j.1365-2958.2007.05940.x;
RA   Wong K.H., Hynes M.J., Todd R.B., Davis M.A.;
RT   "Transcriptional control of nmrA by the bZIP transcription factor MeaB
RT   reveals a new level of nitrogen regulation in Aspergillus nidulans.";
RL   Mol. Microbiol. 66:534-551(2007).
RN   [6] {ECO:0007744|PDB:1K6I, ECO:0007744|PDB:1K6J, ECO:0007744|PDB:1K6X}
RP   X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS) IN COMPLEX WITH NAD.
RX   PubMed=11726498; DOI=10.1093/emboj/20.23.6619;
RA   Stammers D.K., Ren J., Leslie K., Nichols C.E., Lamb H.K., Cocklin S.,
RA   Dodds A., Hawkins A.R.;
RT   "The structure of the negative transcriptional regulator NmrA reveals a
RT   structural superfamily which includes the short-chain
RT   dehydrogenase/reductases.";
RL   EMBO J. 20:6619-6626(2001).
RN   [7] {ECO:0007744|PDB:1TI7}
RP   X-RAY CRYSTALLOGRAPHY (1.7 ANGSTROMS) IN COMPLEXES WITH NADP, FUNCTION, AND
RP   INTERACTION WITH AREA.
RX   PubMed=12764138; DOI=10.1074/jbc.m304104200;
RA   Lamb H.K., Leslie K., Dodds A.L., Nutley M., Cooper A., Johnson C.,
RA   Thompson P., Stammers D.K., Hawkins A.R.;
RT   "The negative transcriptional regulator NmrA discriminates between oxidized
RT   and reduced dinucleotides.";
RL   J. Biol. Chem. 278:32107-32114(2003).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (1.4 ANGSTROMS) OF MUTANT GLY-12/GLY-18 APOPROTEIN,
RP   INTERACTION WITH AREA, FUNCTION, AND MUTAGENESIS OF ASN-12; THR-14; ALA-18;
RP   HIS-37; GLU-193; ASP-195; GLN-202; PHE-204; GLU-263 AND GLU-266.
RX   PubMed=15537757; DOI=10.1110/ps.04958904;
RA   Lamb H.K., Ren J., Park A., Johnson C., Leslie K., Cocklin S., Thompson P.,
RA   Mee C., Cooper A., Stammers D.K., Hawkins A.R.;
RT   "Modulation of the ligand binding properties of the transcription repressor
RT   NmrA by GATA-containing DNA and site-directed mutagenesis.";
RL   Protein Sci. 13:3127-3138(2004).
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) IN COMPLEXES WITH NAD; NADP AND AREA,
RP   AND FUNCTION.
RX   PubMed=18602114; DOI=10.1016/j.jmb.2008.05.077;
RA   Kotaka M., Johnson C., Lamb H.K., Hawkins A.R., Ren J., Stammers D.K.;
RT   "Structural analysis of the recognition of the negative regulator NmrA and
RT   DNA by the zinc finger from the GATA-type transcription factor AreA.";
RL   J. Mol. Biol. 381:373-382(2008).
CC   -!- FUNCTION: May be a redox sensor protein. Has much higher affinity for
CC       NAD(P) than for NAD(P)H. Has similar affinity for NAD and NADP.
CC       Negative transcriptional regulator involved in the post-transcriptional
CC       modulation of the GATA-type transcription factor areA, forming part of
CC       a system controlling nitrogen metabolite repression (By similarity).
CC       Interferes with the interaction between areA and target DNA.
CC       Overexpression leads to areA inhibition. {ECO:0000250,
CC       ECO:0000269|PubMed:12764138, ECO:0000269|PubMed:15537757,
CC       ECO:0000269|PubMed:17854403, ECO:0000269|PubMed:18602114}.
CC   -!- SUBUNIT: Monomer. Interacts with areA. {ECO:0000269|PubMed:11679757,
CC       ECO:0000269|PubMed:11726498, ECO:0000269|PubMed:12764138,
CC       ECO:0000269|PubMed:15537757}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}. Cytoplasm
CC       {ECO:0000269|PubMed:17854403}.
CC   -!- INDUCTION: Up-regulated in nitrogen-sufficient conditions and down-
CC       regulated in nitrogen-limiting conditions.
CC       {ECO:0000269|PubMed:17854403}.
CC   -!- DISRUPTION PHENOTYPE: Displays partial derepression of activities
CC       subject to nitrogen metabolic repression. {ECO:0000269|PubMed:9537404}.
CC   -!- SIMILARITY: Belongs to the NmrA-type oxidoreductase family.
CC       {ECO:0000305}.
CC   -!- CAUTION: Lacks the conserved Tyr residue in position 127 in the active
CC       site triad of Ser-Tyr-Lys that is necessary for dehydrogenase activity,
CC       suggesting that it has no oxidoreductase activity. {ECO:0000305}.
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DR   EMBL; AF041976; AAC39442.1; -; Genomic_DNA.
DR   EMBL; AACD01000141; EAA59190.1; -; Genomic_DNA.
DR   EMBL; BN001302; CBF74025.1; -; Genomic_DNA.
DR   RefSeq; XP_681437.1; XM_676345.1.
DR   PDB; 1K6I; X-ray; 1.80 A; A=1-352.
DR   PDB; 1K6J; X-ray; 1.80 A; A/B=1-352.
DR   PDB; 1K6X; X-ray; 1.50 A; A=1-352.
DR   PDB; 1TI7; X-ray; 1.70 A; A=1-352.
DR   PDB; 1XGK; X-ray; 1.40 A; A=1-352.
DR   PDB; 2VUS; X-ray; 2.60 A; A/B/C/D/E/F/G/H=1-352.
DR   PDB; 2VUT; X-ray; 2.30 A; A/B/C/D/E/F/G/H=1-352.
DR   PDB; 2VUU; X-ray; 2.80 A; A/B/C/D/E/F/G/H=1-352.
DR   PDBsum; 1K6I; -.
DR   PDBsum; 1K6J; -.
DR   PDBsum; 1K6X; -.
DR   PDBsum; 1TI7; -.
DR   PDBsum; 1XGK; -.
DR   PDBsum; 2VUS; -.
DR   PDBsum; 2VUT; -.
DR   PDBsum; 2VUU; -.
DR   AlphaFoldDB; Q5AU62; -.
DR   SMR; Q5AU62; -.
DR   STRING; 162425.CADANIAP00004208; -.
DR   EnsemblFungi; CBF74025; CBF74025; ANIA_08168.
DR   EnsemblFungi; EAA59190; EAA59190; AN8168.2.
DR   GeneID; 2869210; -.
DR   KEGG; ani:AN8168.2; -.
DR   VEuPathDB; FungiDB:AN8168; -.
DR   eggNOG; ENOG502QTW1; Eukaryota.
DR   HOGENOM; CLU_027360_0_0_1; -.
DR   InParanoid; Q5AU62; -.
DR   OMA; PYPLFCM; -.
DR   OrthoDB; 885281at2759; -.
DR   EvolutionaryTrace; Q5AU62; -.
DR   Proteomes; UP000000560; Chromosome II.
DR   Proteomes; UP000005890; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IDA:AspGD.
DR   GO; GO:0051287; F:NAD binding; IDA:UniProtKB.
DR   GO; GO:0070403; F:NAD+ binding; IDA:UniProtKB.
DR   GO; GO:0070401; F:NADP+ binding; IDA:UniProtKB.
DR   GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR   GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR   GO; GO:0090295; P:nitrogen catabolite repression of transcription; IMP:AspGD.
DR   GO; GO:0001081; P:nitrogen catabolite repression of transcription from RNA polymerase II promoter; IMP:AspGD.
DR   GO; GO:0006808; P:regulation of nitrogen utilization; IMP:UniProtKB.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR008030; NmrA-like.
DR   Pfam; PF05368; NmrA; 1.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; NAD; NADP; Nucleus; Oxidoreductase;
KW   Reference proteome; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..352
FT                   /note="Nitrogen metabolite repression protein nmrA"
FT                   /id="PRO_0000393568"
FT   REGION          287..312
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         12..17
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000269|PubMed:12764138,
FT                   ECO:0007744|PDB:1TI7"
FT   BINDING         16..17
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000269|PubMed:11726498,
FT                   ECO:0007744|PDB:1K6X"
FT   BINDING         37
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000269|PubMed:12764138,
FT                   ECO:0007744|PDB:1TI7"
FT   BINDING         80..82
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000269|PubMed:11726498,
FT                   ECO:0007744|PDB:1K6X"
FT   BINDING         80
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000269|PubMed:12764138,
FT                   ECO:0007744|PDB:1TI7"
FT   BINDING         131
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000269|PubMed:11726498,
FT                   ECO:0007744|PDB:1K6X"
FT   BINDING         131
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000269|PubMed:12764138,
FT                   ECO:0007744|PDB:1TI7"
FT   BINDING         153..156
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000269|PubMed:11726498,
FT                   ECO:0007744|PDB:1K6X"
FT   BINDING         153..156
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000269|PubMed:12764138,
FT                   ECO:0007744|PDB:1TI7"
FT   MUTAGEN         12
FT                   /note="N->G: 13-fold increase in the Kd for NAD; 2-fold
FT                   increase in the Kd for NADP and 2-fold increase in the Kd
FT                   for areA; when associated with G-18."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         14
FT                   /note="T->V: 4-fold increase in the Kd for NAD; 7-fold
FT                   increase in the Kd for NADP and no change in the Kd for
FT                   areA."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         18
FT                   /note="A->G: 13-fold increase in the Kd for NAD; 2-fold
FT                   increase in the Kd for NADP and 2-fold increase in the Kd
FT                   for areA; when associated with G-12."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         37
FT                   /note="H->W: 4-fold decrease in the Kd for NAD; 24%
FT                   increase in the Kd for NADP and no change in the Kd for
FT                   areA."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         193
FT                   /note="E->Q: No changes in the Kd for NAD and NADP and
FT                   almost complete loss of affinity for areA; when associated
FT                   with N-195."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         195
FT                   /note="D->N: No changes in the Kd for NAD and NADP and
FT                   almost complete loss of affinity for areA; when associated
FT                   with Q-193."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         202
FT                   /note="Q->E: No changes in the Kd for NAD and NADP and 2-
FT                   fold increase in the Kd for areA; when associated with Y-
FT                   204."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         204
FT                   /note="F->Y: No changes in the Kd for NAD and NADP and 2-
FT                   fold increase in the Kd for areA; when associated with E-
FT                   202."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         263
FT                   /note="E->Q: No changes in the Kd for NAD; NADP and areA;
FT                   when associated with Q-266."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   MUTAGEN         266
FT                   /note="E->Q: No changes in the Kd for NAD; NADP and areA;
FT                   when associated with Q-263."
FT                   /evidence="ECO:0000269|PubMed:15537757"
FT   CONFLICT        238
FT                   /note="R -> L (in Ref. 1; AAC39442)"
FT                   /evidence="ECO:0000305"
FT   STRAND          8..12
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           16..27
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          32..37
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           42..48
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          53..58
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           64..71
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          75..79
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           83..85
FT                   /evidence="ECO:0007829|PDB:1TI7"
FT   HELIX           88..102
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          106..112
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           116..118
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          119..121
FT                   /evidence="ECO:0007829|PDB:1K6I"
FT   TURN            126..128
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           129..139
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          141..143
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          145..150
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           154..156
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          158..161
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          166..169
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          175..181
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          183..185
FT                   /evidence="ECO:0007829|PDB:2VUT"
FT   STRAND          187..190
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           192..206
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           208..211
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          215..218
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          220..223
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           225..236
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          240..244
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           256..269
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           280..282
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   STRAND          283..285
FT                   /evidence="ECO:0007829|PDB:1K6J"
FT   HELIX           319..324
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           331..337
FT                   /evidence="ECO:0007829|PDB:1XGK"
FT   HELIX           339..345
FT                   /evidence="ECO:0007829|PDB:1XGK"
SQ   SEQUENCE   352 AA;  38796 MW;  4E9982FFAE6F1F89 CRC64;
     MAQQKKTIAV VNATGRQAAS LIRVAAAVGH HVRAQVHSLK GLIAEELQAI PNVTLFQGPL
     LNNVPLMDTL FEGAHLAFIN TTSQAGDEIA IGKDLADAAK RAGTIQHYIY SSMPDHSLYG
     PWPAVPMWAP KFTVENYVRQ LGLPSTFVYA GIYNNNFTSL PYPLFQMELM PDGTFEWHAP
     FDPDIPLPWL DAEHDVGPAL LQIFKDGPQK WNGHRIALTF ETLSPVQVCA AFSRALNRRV
     TYVQVPKVEI KVNIPVGYRE QLEAIEVVFG EHKAPYFPLP EFSRPAAGSP KGLGPANGKG
     AGAGMMQGPG GVISQRVTDE ARKLWSGWRD MEEYAREVFP IEEEANGLDW ML
 
 
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