NMS_BOMMX
ID NMS_BOMMX Reviewed; 145 AA.
AC Q4QXT8; Q1HA21; Q1HA22; Q1HA23; Q1HA24; Q1HA25;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 1.
DT 25-MAY-2022, entry version 32.
DE RecName: Full=Neuromedin-S;
DE AltName: Full=Neuromedin-U;
DE Contains:
DE RecName: Full=Neuromedin-S-17;
DE Short=NmS-17;
DE AltName: Full=Neuromedin-U-17;
DE Short=NmU-17;
DE Flags: Precursor;
GN Name=nms;
OS Bombina maxima (Giant fire-bellied toad) (Chinese red belly toad).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Bombinatoridae; Bombina.
OX NCBI_TaxID=161274;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), PROTEIN SEQUENCE OF 120-136,
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND
RP AMIDATION AT ASN-136.
RC TISSUE=Skin secretion;
RX PubMed=15927697; DOI=10.1016/j.regpep.2005.01.007;
RA Lee W.H., Liu S.B., Shen J.H., Jin Y., Lai R., Zhang Y.;
RT "Identification and molecular cloning of a novel neuromedin U analog from
RT the skin secretions of toad Bombina maxima.";
RL Regul. Pept. 129:43-47(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6), PROTEIN SEQUENCE
RP OF 92-134 AND 120-136, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP SPECTROMETRY, AND AMIDATION AT ASN-136.
RC TISSUE=Skin secretion;
RX PubMed=16682011; DOI=10.1016/j.bbrc.2006.04.103;
RA Chen T., Zhou M., Walker B., Harriot P., Mori K., Miyazato M., Kangawa K.,
RA Shaw C.;
RT "Structural and functional analogs of the novel mammalian neuropeptide,
RT neuromedin S (NmS), in the dermal venoms of Eurasian bombinid toads.";
RL Biochem. Biophys. Res. Commun. 345:377-384(2006).
CC -!- FUNCTION: [Neuromedin-S-17]: Stimulates uterine smooth muscle
CC contraction (EC(50)=1.6 nM). Synthetic peptide NmS-17 induces calcium
CC mobilization in CHO cells transfected with either human FM-3/GPR66
CC (EC(50)=0.085 nM) or FM-4/TGR-1 (EC(50)=0.231 nM) NmU/NmS receptors.
CC {ECO:0000269|PubMed:15927697}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:15927697,
CC ECO:0000269|PubMed:16682011}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1; Synonyms=BM1;
CC IsoId=Q4QXT8-1; Sequence=Displayed;
CC Name=2; Synonyms=BM2;
CC IsoId=Q4QXT8-2; Sequence=VSP_043620;
CC Name=3; Synonyms=BM3;
CC IsoId=Q4QXT8-3; Sequence=VSP_043624;
CC Name=4; Synonyms=BM4;
CC IsoId=Q4QXT8-4; Sequence=VSP_043623;
CC Name=5; Synonyms=BM5;
CC IsoId=Q4QXT8-5; Sequence=VSP_043622;
CC Name=6; Synonyms=BM6;
CC IsoId=Q4QXT8-6; Sequence=VSP_043621;
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:15927697, ECO:0000269|PubMed:16682011}.
CC -!- MASS SPECTROMETRY: Mass=1965; Method=FAB;
CC Evidence={ECO:0000269|PubMed:15927697};
CC -!- MASS SPECTROMETRY: Mass=1964; Method=Electrospray; Note=Non-protonated
CC fragment.; Evidence={ECO:0000269|PubMed:16682011};
CC -!- MASS SPECTROMETRY: [Isoform 3]: Mass=3800; Method=Electrospray;
CC Note=The measured mass is of the active peptide NmS-33 generated only
CC from isoform 3. Non-protonated fragment. The measured range is 92-124.;
CC Evidence={ECO:0000269|PubMed:16682011};
CC -!- MISCELLANEOUS: Isoform 3 encodes peptide NmS-33, at positions 92 to
CC 124, as a result of splicing out of an exon that encodes a processing
CC site which generates NmS-17. Similar 33-mers and 36-mers have been
CC isolated from human and from mouse and rat, respectively, although they
CC do not arise by alternative splicing.
CC -!- MISCELLANEOUS: [Isoform 3]: Contains an active peptide, NmS-33, at
CC positions 92-124. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NmU family. {ECO:0000305}.
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DR EMBL; AY652767; AAV74390.1; -; mRNA.
DR EMBL; AM115659; CAJ40970.1; -; mRNA.
DR EMBL; AM115660; CAJ40971.1; -; mRNA.
DR EMBL; AM115661; CAJ40972.1; -; mRNA.
DR EMBL; AM115662; CAJ40973.1; -; mRNA.
DR EMBL; AM115663; CAJ40974.1; -; mRNA.
DR EMBL; AM115664; CAJ40975.1; -; mRNA.
DR AlphaFoldDB; Q4QXT8; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR018070; Neuromedin-U_amidation-site.
DR InterPro; IPR043253; NmS.
DR PANTHER; PTHR32414; PTHR32414; 2.
DR PROSITE; PS00967; NMU; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW Direct protein sequencing; Secreted; Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..89
FT /id="PRO_0000417379"
FT PROPEP 92..117
FT /id="PRO_0000417380"
FT PEPTIDE 120..136
FT /note="Neuromedin-S-17"
FT /id="PRO_0000417381"
FT PROPEP 140..145
FT /id="PRO_0000417382"
FT MOD_RES 136
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:15927697,
FT ECO:0000269|PubMed:16682011"
FT VAR_SEQ 46..62
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043620"
FT VAR_SEQ 83..95
FT /note="Missing (in isoform 6)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043621"
FT VAR_SEQ 84..108
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043622"
FT VAR_SEQ 92..107
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043623"
FT VAR_SEQ 108..119
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043624"
SQ SEQUENCE 145 AA; 16679 MW; CEC0B49956590B04 CRC64;
MRSEKHLLPL PLLLAICCLG TLHLSSGFPQ SVPSYLEGLD IPESERHAFC FSQWTALQDQ
EQIPSFVMDL CSSIYNRMKV NEENNHEIYK RFLFQFSRAK DPSLKIGESQ IATAEYTKRD
SSGIVGRPFF LFRPRNGRKV SINEH
