NMS_BOMOR
ID NMS_BOMOR Reviewed; 145 AA.
AC Q1HA20; Q1HA15; Q1HA16; Q1HA17; Q1HA18; Q1HA19;
DT 16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT 27-JUN-2006, sequence version 1.
DT 25-MAY-2022, entry version 30.
DE RecName: Full=Neuromedin-S;
DE Contains:
DE RecName: Full=Neuromedin-S-17;
DE Short=NmS-17;
DE Flags: Precursor;
GN Name=nms;
OS Bombina orientalis (Oriental fire-bellied toad).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Bombinatoridae; Bombina.
OX NCBI_TaxID=8346;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2; 3; 4; 5 AND 6), PROTEIN SEQUENCE
RP OF 92-134 AND 120-136, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MASS
RP SPECTROMETRY, AND AMIDATION AT ASN-136.
RC TISSUE=Skin secretion;
RX PubMed=16682011; DOI=10.1016/j.bbrc.2006.04.103;
RA Chen T., Zhou M., Walker B., Harriot P., Mori K., Miyazato M., Kangawa K.,
RA Shaw C.;
RT "Structural and functional analogs of the novel mammalian neuropeptide,
RT neuromedin S (NmS), in the dermal venoms of Eurasian bombinid toads.";
RL Biochem. Biophys. Res. Commun. 345:377-384(2006).
CC -!- FUNCTION: [Neuromedin-S-17]: Stimulates uterine smooth muscle
CC contraction (By similarity). Synthetic peptide NmS-17 induces calcium
CC mobilization in CHO cells transfected with either human FM-3/GPR66
CC (EC(50)=0.085 nM) or FM-4/TGR-1 (EC(50)=0.231 nM) NmU/NmS receptors.
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16682011}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Name=1; Synonyms=BO1;
CC IsoId=Q1HA20-1; Sequence=Displayed;
CC Name=2; Synonyms=BO2;
CC IsoId=Q1HA20-2; Sequence=VSP_043616;
CC Name=3; Synonyms=BO3;
CC IsoId=Q1HA20-3; Sequence=VSP_043617;
CC Name=4; Synonyms=BO4;
CC IsoId=Q1HA20-4; Sequence=VSP_043618;
CC Name=5; Synonyms=BO5;
CC IsoId=Q1HA20-5; Sequence=VSP_043614, VSP_043615;
CC Name=6; Synonyms=BO6;
CC IsoId=Q1HA20-6; Sequence=VSP_043619;
CC -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC {ECO:0000269|PubMed:16682011}.
CC -!- MASS SPECTROMETRY: Mass=1964; Method=Electrospray; Note=Non-protonated
CC fragment.; Evidence={ECO:0000269|PubMed:16682011};
CC -!- MASS SPECTROMETRY: [Isoform 6]: Mass=3818; Method=Electrospray;
CC Note=The measured mass is of the active peptide NmS-33 generated only
CC from isoform 6. Non-protonated fragment. The measured range is 92-124.;
CC Evidence={ECO:0000269|PubMed:16682011};
CC -!- MISCELLANEOUS: Isoform 6 encodes peptide NmS-33, at positions 92 to
CC 124, as a result of splicing out of an exon that encodes a processing
CC site which generates NmS-17. Similar 33-mers and 36-mers have been
CC isolated from human and from mouse and rat, respectively, although they
CC do not arise by alternative splicing.
CC -!- MISCELLANEOUS: [Isoform 6]: Contains an active peptide, NmS-33, at
CC positions 92-124. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the NmU family. {ECO:0000305}.
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DR EMBL; AM115665; CAJ40976.1; -; mRNA.
DR EMBL; AM115666; CAJ40977.1; -; mRNA.
DR EMBL; AM115667; CAJ40978.1; -; mRNA.
DR EMBL; AM115668; CAJ40979.1; -; mRNA.
DR EMBL; AM115669; CAJ40980.1; -; mRNA.
DR EMBL; AM115670; CAJ40981.1; -; mRNA.
DR AlphaFoldDB; Q1HA20; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR018070; Neuromedin-U_amidation-site.
DR InterPro; IPR043253; NmS.
DR PANTHER; PTHR32414; PTHR32414; 2.
DR PROSITE; PS00967; NMU; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Amidation; Direct protein sequencing; Secreted;
KW Signal.
FT SIGNAL 1..27
FT /evidence="ECO:0000255"
FT PROPEP 28..89
FT /id="PRO_0000417373"
FT PROPEP 92..117
FT /id="PRO_0000417374"
FT PEPTIDE 120..136
FT /note="Neuromedin-S-17"
FT /id="PRO_5000076891"
FT PROPEP 140..145
FT /id="PRO_0000417375"
FT MOD_RES 136
FT /note="Asparagine amide"
FT /evidence="ECO:0000269|PubMed:16682011"
FT VAR_SEQ 46..62
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043614"
FT VAR_SEQ 83..119
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043615"
FT VAR_SEQ 83..91
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043616"
FT VAR_SEQ 84..108
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043617"
FT VAR_SEQ 92..119
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043618"
FT VAR_SEQ 108..119
FT /note="Missing (in isoform 6)"
FT /evidence="ECO:0000303|PubMed:16682011"
FT /id="VSP_043619"
SQ SEQUENCE 145 AA; 16614 MW; 5C5C4FE355CA853A CRC64;
MRSEKHLPPL PLLLAICCLG TLHPSSGFPQ SVPSYMEALD IPESEKLAFC FSQWTALPDQ
EQIPSFVMDL CSSIYNRMKV NEENNHEIYK RFLFQFSRTK DPSLKTGESQ IATAEYTKRD
SSGIVGRPFF LFRPRNGRKV SINEH
