ID   NMS_BOMVA               Reviewed;         128 AA.
AC   Q1HA14;
DT   16-MAY-2012, integrated into UniProtKB/Swiss-Prot.
DT   27-JUN-2006, sequence version 1.
DT   25-MAY-2022, entry version 28.
DE   RecName: Full=Neuromedin-S;
DE   Contains:
DE     RecName: Full=Neuromedin-S-17;
DE              Short=NmS-17;
DE   Flags: Precursor;
GN   Name=nms;
OS   Bombina variegata (Yellow-bellied toad).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Bombinatoridae; Bombina.
OX   NCBI_TaxID=8348;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 103-119, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, MASS SPECTROMETRY, AND AMIDATION AT ASN-119.
RC   TISSUE=Skin secretion;
RX   PubMed=16682011; DOI=10.1016/j.bbrc.2006.04.103;
RA   Chen T., Zhou M., Walker B., Harriot P., Mori K., Miyazato M., Kangawa K.,
RA   Shaw C.;
RT   "Structural and functional analogs of the novel mammalian neuropeptide,
RT   neuromedin S (NmS), in the dermal venoms of Eurasian bombinid toads.";
RL   Biochem. Biophys. Res. Commun. 345:377-384(2006).
CC   -!- FUNCTION: Neuromedin-S-17: stimulates uterine smooth muscle contraction
CC       (B similarity). Synthetic peptide NmS-17 induces calcium mobilization
CC       in CHO cells transfected with either human FM-3/GPR66 (EC(50)=0.085 nM)
CC       or FM-4/TGR-1 (EC(50)=0.231 nM) NmU/NmS receptors.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16682011}.
CC   -!- TISSUE SPECIFICITY: Expressed by the skin glands.
CC       {ECO:0000269|PubMed:16682011}.
CC   -!- MASS SPECTROMETRY: Mass=1964; Method=Electrospray; Note=Non-protonated
CC       fragment.; Evidence={ECO:0000269|PubMed:16682011};
CC   -!- SIMILARITY: Belongs to the NmU family. {ECO:0000305}.
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DR   EMBL; AM115671; CAJ40982.1; -; mRNA.
DR   AlphaFoldDB; Q1HA14; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR018070; Neuromedin-U_amidation-site.
DR   InterPro; IPR043253; NmS.
DR   PANTHER; PTHR32414; PTHR32414; 2.
DR   PROSITE; PS00967; NMU; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Secreted; Signal.
FT   SIGNAL          1..27
FT                   /evidence="ECO:0000255"
FT   PROPEP          28..72
FT                   /id="PRO_0000417376"
FT   PROPEP          75..100
FT                   /id="PRO_0000417377"
FT   PEPTIDE         103..119
FT                   /note="Neuromedin-S-17"
FT                   /id="PRO_5000076903"
FT   PROPEP          123..128
FT                   /id="PRO_0000417378"
FT   MOD_RES         119
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:16682011"
SQ   SEQUENCE   128 AA;  14586 MW;  C6616FCBB18877B6 CRC64;
     MRSEKHLLPL PPLLAICCLG TLHLSSGFPQ SVPSYLEGLD IPESEIPSFV MDLCSSIYNR
     MKVNEENNHE IYKRFLFQFS RAKDPSLKIG ESQIATAEYT KRDSSGIVGR PFFLFRPRNG
     RKVSINEH