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NMT1_PONAB
ID   NMT1_PONAB              Reviewed;         496 AA.
AC   Q5RAF3;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   21-DEC-2004, sequence version 1.
DT   03-AUG-2022, entry version 72.
DE   RecName: Full=Glycylpeptide N-tetradecanoyltransferase 1;
DE            EC=2.3.1.97 {ECO:0000250|UniProtKB:P30419};
DE   AltName: Full=Myristoyl-CoA:protein N-myristoyltransferase 1;
DE            Short=NMT 1;
DE            Short=Type I N-myristoyltransferase;
DE   AltName: Full=Peptide N-myristoyltransferase 1;
GN   Name=NMT1;
OS   Pongo abelii (Sumatran orangutan) (Pongo pygmaeus abelii).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Pongo.
OX   NCBI_TaxID=9601;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RG   The German cDNA consortium;
RL   Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Adds a myristoyl group to the N-terminal glycine residue of
CC       certain cellular and viral proteins. Also able to mediate N-terminal
CC       lysine myristoylation of proteins: catalyzes myristoylation of ARF6 on
CC       both 'Gly-2' and 'Lys-3'. Lysine myristoylation is required to maintain
CC       ARF6 on membranes during the GTPase cycle.
CC       {ECO:0000250|UniProtKB:P30419}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H(+) +
CC         N-tetradecanoylglycyl-[protein]; Xref=Rhea:RHEA:15521, Rhea:RHEA-
CC         COMP:12666, Rhea:RHEA-COMP:12667, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57385, ChEBI:CHEBI:64723,
CC         ChEBI:CHEBI:133050; EC=2.3.1.97;
CC         Evidence={ECO:0000250|UniProtKB:P30419};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-terminal glycyl-L-lysyl-[protein] + tetradecanoyl-CoA = CoA
CC         + H(+) + N-terminal glycyl-(N(6)-tetradecanoyl)-L-lysyl-[protein];
CC         Xref=Rhea:RHEA:70671, Rhea:RHEA-COMP:17947, Rhea:RHEA-COMP:17948,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57385,
CC         ChEBI:CHEBI:189855, ChEBI:CHEBI:189856;
CC         Evidence={ECO:0000250|UniProtKB:P30419};
CC       PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70672;
CC         Evidence={ECO:0000250|UniProtKB:P30419};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:P30419}.
CC       Cytoplasm, cytosol {ECO:0000250|UniProtKB:P30419}. Membrane
CC       {ECO:0000250|UniProtKB:P30419}; Peripheral membrane protein
CC       {ECO:0000250|UniProtKB:P30419}. Note=Copurifies with ribosomes.
CC       {ECO:0000250|UniProtKB:P30419}.
CC   -!- SIMILARITY: Belongs to the NMT family. {ECO:0000305}.
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DR   EMBL; CR859064; CAH91257.1; -; mRNA.
DR   RefSeq; NP_001127395.1; NM_001133923.2.
DR   AlphaFoldDB; Q5RAF3; -.
DR   SMR; Q5RAF3; -.
DR   STRING; 9601.ENSPPYP00000009343; -.
DR   GeneID; 100174462; -.
DR   KEGG; pon:100174462; -.
DR   CTD; 4836; -.
DR   eggNOG; KOG2779; Eukaryota.
DR   InParanoid; Q5RAF3; -.
DR   OrthoDB; 1025421at2759; -.
DR   Proteomes; UP000001595; Unplaced.
DR   GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR   GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR   GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0004379; F:glycylpeptide N-tetradecanoyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0018030; F:peptidyl-lysine N6-myristoyltransferase activity; ISS:UniProtKB.
DR   GO; GO:0018008; P:N-terminal peptidyl-glycine N-myristoylation; ISS:UniProtKB.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000903; NMT.
DR   InterPro; IPR022677; NMT_C.
DR   InterPro; IPR022678; NMT_CS.
DR   InterPro; IPR022676; NMT_N.
DR   PANTHER; PTHR11377; PTHR11377; 1.
DR   Pfam; PF01233; NMT; 1.
DR   Pfam; PF02799; NMT_C; 1.
DR   PIRSF; PIRSF015892; N-myristl_transf; 1.
DR   SUPFAM; SSF55729; SSF55729; 2.
DR   PROSITE; PS00975; NMT_1; 1.
DR   PROSITE; PS00976; NMT_2; 1.
PE   2: Evidence at transcript level;
KW   Acyltransferase; Cytoplasm; Membrane; Phosphoprotein; Reference proteome;
KW   Transferase.
FT   CHAIN           1..496
FT                   /note="Glycylpeptide N-tetradecanoyltransferase 1"
FT                   /id="PRO_0000064223"
FT   REGION          1..82
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         118
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         119
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         120
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         247
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         248
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         249
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         250
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         256
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         258
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         259
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   BINDING         260
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   MOD_RES         31
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:O70310"
FT   MOD_RES         47
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
FT   MOD_RES         83
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0000250|UniProtKB:P30419"
SQ   SEQUENCE   496 AA;  56746 MW;  A8D8AA6834B81680 CRC64;
     MADESETAVK PPAPPLPQMM EGNGNGHEHC SDCENEEDNS YNRGGLSPAN DTGAKKKKKK
     QKKKKEKGSE TDSAQDQPVK MNSLPAERIQ EIQKAIELFS VGQGPAKTME EASKRSYQFW
     DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY TLPQGSTWDA LDLGDRGVLK ELYTLLNENY
     VEDDDNMFRF DYSPEFLLWA LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK
     KMVEINFLCV HKKLRSKRVA PVLIREITRR IHLEGVFQAV YTAGVVLPKP VGTCRYWHRS
     LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP METKDIPVVH QLLTRYLKQF
     HLTPVMSQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF YTLPSTIMNH PTHKSLKAAY
     SFYNVHTQTP LLDLMSDALV LAKMKGFDVF NALDLMENKT FLEKLKFGIG DGNLQYYLYN
     WKCPSMGAEK VGLVLQ
 
 
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