ID   NMT2_ARATH              Reviewed;         430 AA.
AC   Q94L32; O80586;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Putative glycylpeptide N-tetradecanoyltransferase 2;
DE            EC=2.3.1.97;
DE   AltName: Full=Myristoyl-CoA:protein N-myristoyltransferase 2;
DE            Short=NMT 2;
DE            Short=Type I N-myristoyltransferase 2;
DE   AltName: Full=Peptide N-myristoyltransferase 2;
GN   Name=NMT2; OrderedLocusNames=At2g44170; ORFNames=F6E13.30;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=12912986; DOI=10.1074/jbc.m307321200;
RA   Boisson B., Giglione C., Meinnel T.;
RT   "Unexpected protein families including cell defense components feature in
RT   the N-myristoylome of a higher eukaryote.";
RL   J. Biol. Chem. 278:43418-43429(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10617197; DOI=10.1038/45471;
RA   Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA   Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA   Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA   Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA   Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA   Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA   Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT   "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL   Nature 402:761-768(1999).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
CC   -!- FUNCTION: May add a myristoyl group to the N-terminal glycine residue
CC       of certain cellular proteins. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H(+) +
CC         N-tetradecanoylglycyl-[protein]; Xref=Rhea:RHEA:15521, Rhea:RHEA-
CC         COMP:12666, Rhea:RHEA-COMP:12667, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:57287, ChEBI:CHEBI:57385, ChEBI:CHEBI:64723,
CC         ChEBI:CHEBI:133050; EC=2.3.1.97;
CC   -!- SIMILARITY: Belongs to the NMT family. {ECO:0000305}.
CC   -!- CAUTION: Could be the product of a pseudogene. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC23421.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AF250957; AAK49038.1; -; mRNA.
DR   EMBL; AC004005; AAC23421.2; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002685; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; T00697; T00697.
DR   AlphaFoldDB; Q94L32; -.
DR   SMR; Q94L32; -.
DR   PeptideAtlas; Q94L32; -.
DR   PRIDE; Q94L32; -.
DR   Araport; AT2G44170; -.
DR   Proteomes; UP000006548; Chromosome 2.
DR   ExpressionAtlas; Q94L32; baseline and differential.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0004379; F:glycylpeptide N-tetradecanoyltransferase activity; IBA:GO_Central.
DR   GO; GO:0018008; P:N-terminal peptidyl-glycine N-myristoylation; IBA:GO_Central.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   InterPro; IPR000903; NMT.
DR   InterPro; IPR022677; NMT_C.
DR   InterPro; IPR022678; NMT_CS.
DR   InterPro; IPR022676; NMT_N.
DR   PANTHER; PTHR11377; PTHR11377; 1.
DR   Pfam; PF01233; NMT; 1.
DR   Pfam; PF02799; NMT_C; 1.
DR   PIRSF; PIRSF015892; N-myristl_transf; 1.
DR   SUPFAM; SSF55729; SSF55729; 2.
DR   PROSITE; PS00975; NMT_1; 1.
PE   5: Uncertain;
KW   Acyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..430
FT                   /note="Putative glycylpeptide N-tetradecanoyltransferase 2"
FT                   /id="PRO_0000064232"
FT   ACT_SITE        430
FT                   /note="Proton acceptor; via carboxylate"
FT                   /evidence="ECO:0000250"
FT   BINDING         47..50
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P14743"
FT   BINDING         181..183
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P14743"
FT   BINDING         189..193
FT                   /ligand="tetradecanoyl-CoA"
FT                   /ligand_id="ChEBI:CHEBI:57385"
FT                   /evidence="ECO:0000250|UniProtKB:P14743"
SQ   SEQUENCE   430 AA;  49628 MW;  08F2333ED0E23035 CRC64;
     MSDPKLKPVE DALVTVAKSS QIQLAKDDTS GGTIVGRLLQ CQYSKTHKFW ETQPVEQFKD
     IQDTSLPEGP IEPATLVSEV KQEPYNLLGQ FEWTICDMNS DDMCLEMYNF LKENSPDDQQ
     IKYEYSKEYL RWALCPPGYY QSWHIGVRVK TSKKLIAFIC GRPTRIRVRD EVVKMAKVNS
     LCVHKKLRSK GLAPLMIKEL TRRVKLQNIW QAAYTSSHIL SRPVTTSRDW VRMLNPKKLI
     DVGLTRLRDR MTMSRTVKLY KLPDAPITPG FREMERRDVP AVTALLRNYL SQFAVATDFD
     DNDVKHWLLP RENIVYSYVV VSPETHDVTD FCSFCNSSIT IPGNRKYTTL ECAYACCNVA
     TLTSLSQLVN DALIVSKQKG FDVFYASDVM QNESFLKELR FYPLCRQSHY YLYNYRLRNA
     LKPSELGLIL