NMT_CRYNJ
ID NMT_CRYNJ Reviewed; 493 AA.
AC P0CP20; Q55HW5; Q5K7E9;
DT 28-JUN-2011, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 46.
DE RecName: Full=Glycylpeptide N-tetradecanoyltransferase;
DE EC=2.3.1.97;
DE AltName: Full=Myristoyl-CoA:protein N-myristoyltransferase;
DE Short=NMT;
DE AltName: Full=Peptide N-myristoyltransferase;
GN OrderedLocusNames=CNN00080;
OS Cryptococcus neoformans var. neoformans serotype D (strain JEC21 / ATCC
OS MYA-565) (Filobasidiella neoformans).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Tremellomycetes;
OC Tremellales; Cryptococcaceae; Cryptococcus;
OC Cryptococcus neoformans species complex.
OX NCBI_TaxID=214684;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEC21 / ATCC MYA-565;
RX PubMed=15653466; DOI=10.1126/science.1103773;
RA Loftus B.J., Fung E., Roncaglia P., Rowley D., Amedeo P., Bruno D.,
RA Vamathevan J., Miranda M., Anderson I.J., Fraser J.A., Allen J.E.,
RA Bosdet I.E., Brent M.R., Chiu R., Doering T.L., Donlin M.J., D'Souza C.A.,
RA Fox D.S., Grinberg V., Fu J., Fukushima M., Haas B.J., Huang J.C.,
RA Janbon G., Jones S.J.M., Koo H.L., Krzywinski M.I., Kwon-Chung K.J.,
RA Lengeler K.B., Maiti R., Marra M.A., Marra R.E., Mathewson C.A.,
RA Mitchell T.G., Pertea M., Riggs F.R., Salzberg S.L., Schein J.E.,
RA Shvartsbeyn A., Shin H., Shumway M., Specht C.A., Suh B.B., Tenney A.,
RA Utterback T.R., Wickes B.L., Wortman J.R., Wye N.H., Kronstad J.W.,
RA Lodge J.K., Heitman J., Davis R.W., Fraser C.M., Hyman R.W.;
RT "The genome of the basidiomycetous yeast and human pathogen Cryptococcus
RT neoformans.";
RL Science 307:1321-1324(2005).
CC -!- FUNCTION: Adds a myristoyl group to the N-terminal glycine residue of
CC certain cellular proteins. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-terminal glycyl-[protein] + tetradecanoyl-CoA = CoA + H(+) +
CC N-tetradecanoylglycyl-[protein]; Xref=Rhea:RHEA:15521, Rhea:RHEA-
CC COMP:12666, Rhea:RHEA-COMP:12667, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57385, ChEBI:CHEBI:64723,
CC ChEBI:CHEBI:133050; EC=2.3.1.97;
CC -!- SUBUNIT: Monomer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NMT family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAW47000.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AE017356; AAW47000.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_568517.1; XM_568517.1.
DR AlphaFoldDB; P0CP20; -.
DR SMR; P0CP20; -.
DR STRING; 5207.AAW47000; -.
DR PaxDb; P0CP20; -.
DR EnsemblFungi; AAW47000; AAW47000; CNN00080.
DR GeneID; 3255415; -.
DR KEGG; cne:CNN00080; -.
DR eggNOG; KOG2779; Eukaryota.
DR InParanoid; P0CP20; -.
DR OrthoDB; 1025421at2759; -.
DR Proteomes; UP000002149; Chromosome 14.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IEA:EnsemblFungi.
DR GO; GO:0004379; F:glycylpeptide N-tetradecanoyltransferase activity; IBA:GO_Central.
DR GO; GO:0018008; P:N-terminal peptidyl-glycine N-myristoylation; IBA:GO_Central.
DR InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR InterPro; IPR000903; NMT.
DR InterPro; IPR022677; NMT_C.
DR InterPro; IPR022678; NMT_CS.
DR InterPro; IPR022676; NMT_N.
DR PANTHER; PTHR11377; PTHR11377; 1.
DR Pfam; PF01233; NMT; 1.
DR Pfam; PF02799; NMT_C; 1.
DR PIRSF; PIRSF015892; N-myristl_transf; 1.
DR SUPFAM; SSF55729; SSF55729; 2.
DR PROSITE; PS00975; NMT_1; 1.
DR PROSITE; PS00976; NMT_2; 1.
PE 3: Inferred from homology;
KW Acyltransferase; Cytoplasm; Reference proteome; Transferase.
FT CHAIN 1..493
FT /note="Glycylpeptide N-tetradecanoyltransferase"
FT /id="PRO_0000064238"
FT REGION 53..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 493
FT /note="Proton acceptor; via carboxylate"
FT /evidence="ECO:0000250"
FT BINDING 45..48
FT /ligand="tetradecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:57385"
FT /evidence="ECO:0000250|UniProtKB:P14743"
FT BINDING 182..184
FT /ligand="tetradecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:57385"
FT /evidence="ECO:0000250|UniProtKB:P14743"
FT BINDING 190..194
FT /ligand="tetradecanoyl-CoA"
FT /ligand_id="ChEBI:CHEBI:57385"
FT /evidence="ECO:0000250|UniProtKB:P14743"
SQ SEQUENCE 493 AA; 54964 MW; CC82641C5B7CFB02 CRC64;
MDSSDSKAAT DEEIRRALKA ADLMKILDGK IALGNKSGTK NLGEHKFWKT QPVPQITGSG
APAPIEEGPI DDPKTPADVR QEPGVLPAGF EWSTIDINDE EQSKEVYVLL CENYVEDDDA
MFRFNYSREF LLWALTAPGY LPDWHIGVRV QKTKKLVAFI SGIKIDIRVR AKTFPAAEIN
FLCVHKKLRS KRLAPVLIKE VTRRVNLTNI WQAIYTAGVI LPTPIGTCRY FHRNLNPPKL
VDIGFSPLPR GSTIARLVQQ YSVPSHPRIP GFREMKKEDV PQVGALLRRY LDRFDVAQAF
RDDDEVEHWL LSGQGKEVGG RRVEQVVWAY VVEDPTTHRI TDLISFYALP STIMKHPKHN
LLNAAYMFYY ATDVVFPPSS SSANSDVDVD ANAGESSVAA VGTGGEDAKT KKKLETRLNA
LTADILIIAK QAGFDVFNAL TLLDNNMFLQ EQKFGPGDGY LNYYLYNWNC APIDGGHHST
TAKQGSKIGV VML