NMUR2_HUMAN
ID NMUR2_HUMAN Reviewed; 415 AA.
AC Q9GZQ4; Q7LC54; Q96AM5; Q9NRA6;
DT 06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 2.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Neuromedin-U receptor 2;
DE Short=NMU-R2;
DE AltName: Full=G-protein coupled receptor FM-4;
DE AltName: Full=G-protein coupled receptor TGR-1;
GN Name=NMUR2; Synonyms=NMU2R, TGR1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANT THR-395.
RX PubMed=10887190; DOI=10.1074/jbc.m004261200;
RA Hosoya M., Moriya T., Kawamata Y., Ohkubo S., Fujii R., Matsui H.,
RA Shintani Y., Fukusumi S., Habata Y., Hinuma S., Onda H., Nishimura O.,
RA Fujino M.;
RT "Identification and functional characterization of a novel subtype of
RT neuromedin U receptor.";
RL J. Biol. Chem. 275:29528-29532(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND VARIANT
RP THR-395.
RX PubMed=10899166; DOI=10.1074/jbc.m004613200;
RA Raddatz R., Wilson A.E., Artymyshyn R., Bonini J.A., Borowsky B.,
RA Boteju L.W., Zhou S., Kouranova E.V., Nagorny R., Guevarra M.S., Dai M.,
RA Lerman G.S., Vaysse P.J., Branchek T.A., Gerald C., Forray C., Adham N.;
RT "Identification and characterization of two neuromedin U receptors
RT differentially expressed in peripheral tissues and the central nervous
RT system.";
RL J. Biol. Chem. 275:32452-32459(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND VARIANT THR-395.
RX PubMed=11010960; DOI=10.1074/jbc.c000522200;
RA Shan L., Qiao X., Crona J.H., Behan J., Wang S., Laz T., Bayne M.,
RA Gustafson E.L., Monsma F.J. Jr., Hedrick J.A.;
RT "Identification of a novel neuromedin U receptor subtype expressed in the
RT central nervous system.";
RL J. Biol. Chem. 275:39482-39486(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15372022; DOI=10.1038/nature02919;
RA Schmutz J., Martin J., Terry A., Couronne O., Grimwood J., Lowry S.,
RA Gordon L.A., Scott D., Xie G., Huang W., Hellsten U., Tran-Gyamfi M.,
RA She X., Prabhakar S., Aerts A., Altherr M., Bajorek E., Black S.,
RA Branscomb E., Caoile C., Challacombe J.F., Chan Y.M., Denys M.,
RA Detter J.C., Escobar J., Flowers D., Fotopulos D., Glavina T., Gomez M.,
RA Gonzales E., Goodstein D., Grigoriev I., Groza M., Hammon N., Hawkins T.,
RA Haydu L., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A.,
RA Lopez F., Lou Y., Martinez D., Medina C., Morgan J., Nandkeshwar R.,
RA Noonan J.P., Pitluck S., Pollard M., Predki P., Priest J., Ramirez L.,
RA Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., Thayer N.,
RA Tice H., Tsai M., Ustaszewska A., Vo N., Wheeler J., Wu K., Yang J.,
RA Dickson M., Cheng J.-F., Eichler E.E., Olsen A., Pennacchio L.A.,
RA Rokhsar D.S., Richardson P., Lucas S.M., Myers R.M., Rubin E.M.;
RT "The DNA sequence and comparative analysis of human chromosome 5.";
RL Nature 431:268-274(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT THR-395.
RC TISSUE=Colon, and Placenta;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 4-415, TISSUE SPECIFICITY, AND VARIANT
RP THR-395.
RX PubMed=10894543; DOI=10.1038/35017610;
RA Howard A.D., Wang R., Pong S.-S., Mellin T.N., Strack A., Guan X.-M.,
RA Zeng Z., Williams D.L., Feighner S.D., Nunes C.N., Murphy B., Stair J.N.,
RA Yu H., Jiang Q., Clements M.K., Tan C.P., Mckee K.K., Hreniuk D.L.,
RA Mcdonald T.P., Lynch K.R., Evans J.F., Austin C.P., Caskey T.,
RA van der Ploeg L.H.T., Liu Q.;
RT "Identification of receptors for neuromedin U and its role in feeding.";
RL Nature 406:70-74(2000).
CC -!- FUNCTION: Receptor for the neuromedin-U and neuromedin-S neuropeptides.
CC {ECO:0000250, ECO:0000269|PubMed:10899166}.
CC -!- INTERACTION:
CC Q9GZQ4; Q6UY14-3: ADAMTSL4; NbExp=3; IntAct=EBI-10303844, EBI-10173507;
CC Q9GZQ4; P15884: TCF4; NbExp=3; IntAct=EBI-10303844, EBI-533224;
CC Q9GZQ4; Q12933: TRAF2; NbExp=3; IntAct=EBI-10303844, EBI-355744;
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the CNS, particularly in
CC the medulla oblongata, pontine reticular formation, spinal cord, and
CC thalamus. High level in testis whereas lower levels are present in a
CC variety of peripheral tissues including the gastrointestinal tract,
CC genitourinary tract, liver, pancreas, adrenal gland, thyroid gland,
CC lung, trachea, spleen and thymus. {ECO:0000269|PubMed:10887190,
CC ECO:0000269|PubMed:10894543, ECO:0000269|PubMed:10899166,
CC ECO:0000269|PubMed:11010960}.
CC -!- SIMILARITY: Belongs to the G-protein coupled receptor 1 family.
CC {ECO:0000255|PROSITE-ProRule:PRU00521}.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-4 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB13721.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB041228; BAB13721.1; ALT_INIT; mRNA.
DR EMBL; AF272363; AAG24794.1; -; mRNA.
DR EMBL; AF292402; AAG03064.1; -; mRNA.
DR EMBL; AC008571; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC016938; AAH16938.1; -; mRNA.
DR EMBL; BC067776; AAH67776.1; -; mRNA.
DR EMBL; AF242874; AAF82755.1; -; mRNA.
DR CCDS; CCDS4321.1; -.
DR RefSeq; NP_064552.3; NM_020167.4.
DR AlphaFoldDB; Q9GZQ4; -.
DR SMR; Q9GZQ4; -.
DR BioGRID; 121250; 30.
DR IntAct; Q9GZQ4; 22.
DR STRING; 9606.ENSP00000255262; -.
DR BindingDB; Q9GZQ4; -.
DR ChEMBL; CHEMBL1075144; -.
DR DrugCentral; Q9GZQ4; -.
DR GuidetoPHARMACOLOGY; 299; -.
DR GlyGen; Q9GZQ4; 3 sites.
DR iPTMnet; Q9GZQ4; -.
DR PhosphoSitePlus; Q9GZQ4; -.
DR BioMuta; NMUR2; -.
DR DMDM; 311033403; -.
DR MassIVE; Q9GZQ4; -.
DR PaxDb; Q9GZQ4; -.
DR PeptideAtlas; Q9GZQ4; -.
DR PRIDE; Q9GZQ4; -.
DR Antibodypedia; 16394; 195 antibodies from 29 providers.
DR DNASU; 56923; -.
DR Ensembl; ENST00000255262.4; ENSP00000255262.4; ENSG00000132911.5.
DR GeneID; 56923; -.
DR KEGG; hsa:56923; -.
DR MANE-Select; ENST00000255262.4; ENSP00000255262.4; NM_020167.5; NP_064552.3.
DR UCSC; uc003luv.3; human.
DR CTD; 56923; -.
DR DisGeNET; 56923; -.
DR GeneCards; NMUR2; -.
DR HGNC; HGNC:16454; NMUR2.
DR HPA; ENSG00000132911; Tissue enriched (brain).
DR MIM; 605108; gene.
DR neXtProt; NX_Q9GZQ4; -.
DR OpenTargets; ENSG00000132911; -.
DR PharmGKB; PA31664; -.
DR VEuPathDB; HostDB:ENSG00000132911; -.
DR eggNOG; KOG3656; Eukaryota.
DR GeneTree; ENSGT01050000244813; -.
DR HOGENOM; CLU_009579_6_5_1; -.
DR InParanoid; Q9GZQ4; -.
DR OMA; YTVMTYV; -.
DR OrthoDB; 890529at2759; -.
DR PhylomeDB; Q9GZQ4; -.
DR TreeFam; TF318522; -.
DR PathwayCommons; Q9GZQ4; -.
DR Reactome; R-HSA-375276; Peptide ligand-binding receptors.
DR Reactome; R-HSA-416476; G alpha (q) signalling events.
DR Reactome; R-HSA-418594; G alpha (i) signalling events.
DR SignaLink; Q9GZQ4; -.
DR SIGNOR; Q9GZQ4; -.
DR BioGRID-ORCS; 56923; 9 hits in 1058 CRISPR screens.
DR GeneWiki; Neuromedin_U_receptor_2; -.
DR GenomeRNAi; 56923; -.
DR Pharos; Q9GZQ4; Tchem.
DR PRO; PR:Q9GZQ4; -.
DR Proteomes; UP000005640; Chromosome 5.
DR RNAct; Q9GZQ4; protein.
DR Bgee; ENSG00000132911; Expressed in tibialis anterior and 56 other tissues.
DR Genevisible; Q9GZQ4; HS.
DR GO; GO:0016021; C:integral component of membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0004930; F:G protein-coupled receptor activity; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0005229; F:intracellular calcium activated chloride channel activity; IDA:UniProtKB.
DR GO; GO:0042924; F:neuromedin U binding; IDA:UniProtKB.
DR GO; GO:0001607; F:neuromedin U receptor activity; IDA:UniProtKB.
DR GO; GO:0008188; F:neuropeptide receptor activity; IBA:GO_Central.
DR GO; GO:0050482; P:arachidonic acid secretion; IDA:UniProtKB.
DR GO; GO:0006816; P:calcium ion transport; IDA:UniProtKB.
DR GO; GO:0019722; P:calcium-mediated signaling; NAS:UniProtKB.
DR GO; GO:0007267; P:cell-cell signaling; TAS:ProtInc.
DR GO; GO:0007417; P:central nervous system development; IEP:UniProtKB.
DR GO; GO:0007631; P:feeding behavior; TAS:ProtInc.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR GO; GO:0007625; P:grooming behavior; IEA:Ensembl.
DR GO; GO:0048016; P:inositol phosphate-mediated signaling; IDA:UniProtKB.
DR GO; GO:0007218; P:neuropeptide signaling pathway; IDA:UniProtKB.
DR GO; GO:0007200; P:phospholipase C-activating G protein-coupled receptor signaling pathway; IDA:UniProtKB.
DR GO; GO:0007204; P:positive regulation of cytosolic calcium ion concentration; IDA:UniProtKB.
DR GO; GO:0002023; P:reduction of food intake in response to dietary excess; IEA:Ensembl.
DR GO; GO:0051930; P:regulation of sensory perception of pain; IEA:Ensembl.
DR GO; GO:0006940; P:regulation of smooth muscle contraction; NAS:UniProtKB.
DR GO; GO:0048265; P:response to pain; IEA:Ensembl.
DR InterPro; IPR000276; GPCR_Rhodpsn.
DR InterPro; IPR017452; GPCR_Rhodpsn_7TM.
DR InterPro; IPR005390; NeuromedU_rcpt.
DR InterPro; IPR005392; NeuromedU_rcpt_2.
DR InterPro; IPR045561; NMU-R2_C.
DR Pfam; PF00001; 7tm_1; 1.
DR Pfam; PF19285; NmU-R2_C_term; 1.
DR PRINTS; PR00237; GPCRRHODOPSN.
DR PRINTS; PR01565; NEUROMEDINUR.
DR PRINTS; PR01567; NEUROMEDNU2R.
DR SMART; SM01381; 7TM_GPCR_Srsx; 1.
DR PROSITE; PS00237; G_PROTEIN_RECEP_F1_1; 1.
DR PROSITE; PS50262; G_PROTEIN_RECEP_F1_2; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Disulfide bond; G-protein coupled receptor; Glycoprotein;
KW Membrane; Receptor; Reference proteome; Transducer; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..415
FT /note="Neuromedin-U receptor 2"
FT /id="PRO_0000069910"
FT TOPO_DOM 1..49
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical; Name=1"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical; Name=2"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..123
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 124..146
FT /note="Helical; Name=3"
FT /evidence="ECO:0000255"
FT TOPO_DOM 147..165
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 166..186
FT /note="Helical; Name=4"
FT /evidence="ECO:0000255"
FT TOPO_DOM 187..214
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical; Name=5"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 266..286
FT /note="Helical; Name=6"
FT /evidence="ECO:0000255"
FT TOPO_DOM 287..301
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 302..322
FT /note="Helical; Name=7"
FT /evidence="ECO:0000255"
FT TOPO_DOM 323..415
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 9
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 27
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 194
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 119..204
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00521"
FT VARIANT 298
FT /note="S -> T (in dbSNP:rs4958535)"
FT /id="VAR_023941"
FT VARIANT 315
FT /note="F -> L (in dbSNP:rs1895245)"
FT /id="VAR_023942"
FT VARIANT 383
FT /note="P -> L (in dbSNP:rs4958532)"
FT /id="VAR_032770"
FT VARIANT 388
FT /note="M -> V (in dbSNP:rs4958531)"
FT /id="VAR_023943"
FT VARIANT 395
FT /note="A -> T (in dbSNP:rs1363422)"
FT /evidence="ECO:0000269|PubMed:10887190,
FT ECO:0000269|PubMed:10894543, ECO:0000269|PubMed:10899166,
FT ECO:0000269|PubMed:11010960, ECO:0000269|PubMed:15489334"
FT /id="VAR_023944"
FT CONFLICT 204
FT /note="C -> F (in Ref. 5; AAH16938)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 415 AA; 47696 MW; C2BACD84A812890F CRC64;
MSGMEKLQNA SWIYQQKLED PFQKHLNSTE EYLAFLCGPR RSHFFLPVSV VYVPIFVVGV
IGNVLVCLVI LQHQAMKTPT NYYLFSLAVS DLLVLLLGMP LEVYEMWRNY PFLFGPVGCY
FKTALFETVC FASILSITTV SVERYVAILH PFRAKLQSTR RRALRILGIV WGFSVLFSLP
NTSIHGIKFH YFPNGSLVPG SATCTVIKPM WIYNFIIQVT SFLFYLLPMT VISVLYYLMA
LRLKKDKSLE ADEGNANIQR PCRKSVNKML FVLVLVFAIC WAPFHIDRLF FSFVEEWSES
LAAVFNLVHV VSGVFFYLSS AVNPIIYNLL SRRFQAAFQN VISSFHKQWH SQHDPQLPPA
QRNIFLTECH FVELTEDIGP QFPCQSSMHN SHLPAALSSE QMSRTNYQSF HFNKT
