ID   NMU_RANCA               Reviewed;         175 AA.
AC   P81872; Q800H8; Q8JFY6; Q8JFY7;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2006, sequence version 2.
DT   25-MAY-2022, entry version 66.
DE   RecName: Full=Neuromedin-U;
DE   Contains:
DE     RecName: Full=Neuromedin-U-23;
DE              Short=NmU-23;
DE   Flags: Precursor;
GN   Name=nmu;
OS   Ranoidea caerulea (Green tree frog) (Litoria caerulea).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Neobatrachia; Hyloidea; Hylidae; Pelodryadinae; Ranoidea.
OX   NCBI_TaxID=30344;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Skin;
RX   PubMed=12413397; DOI=10.1042/bj20021343;
RA   Chen T., Farragher S.M., Bjourson A.J., Orr D.F., Rao P., Shaw C.;
RT   "Granular gland transcriptomes in stimulated amphibian skin secretions.";
RL   Biochem. J. 371:125-130(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 54-173 (ISOFORM 2).
RA   Farragher S.M., Bjourson A.J., Tracey J., Shaw C.;
RT   "Neuromedin U mRNA isoforms from vertebrates.";
RL   Submitted (OCT-2002) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   PROTEIN SEQUENCE OF 145-167, AMIDATION AT ASN-167, AND SYNTHESIS.
RC   TISSUE=Skin secretion;
RX   PubMed=10671478; DOI=10.1074/jbc.275.7.4549;
RA   Salmon A.L., Johnsen A.H., Bienert M., McMurray G., Nandha K.A.,
RA   Bloom S.R., Shaw C.;
RT   "Isolation, structural characterization and bioactivity of a novel
RT   neuromedin U analog from the defensive skin secretion of the Australasian
RT   tree frog Litoria caerulea.";
RL   J. Biol. Chem. 275:4549-4554(2000).
CC   -!- FUNCTION: Stimulates uterine smooth muscle contraction and causes
CC       selective vasoconstriction.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=3;
CC       Name=1;
CC         IsoId=P81872-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P81872-2; Sequence=VSP_021779;
CC       Name=3;
CC         IsoId=P81872-3; Sequence=VSP_021780;
CC   -!- TISSUE SPECIFICITY: Skin.
CC   -!- SIMILARITY: Belongs to the NmU family. {ECO:0000305}.
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DR   EMBL; AJ457825; CAD29882.1; -; mRNA.
DR   EMBL; AJ457826; CAD29883.1; -; mRNA.
DR   EMBL; AJ510131; CAD52850.1; -; mRNA.
DR   AlphaFoldDB; P81872; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0005179; F:hormone activity; IEA:UniProtKB-KW.
DR   GO; GO:0042922; F:neuromedin U receptor binding; IEA:InterPro.
DR   GO; GO:0006940; P:regulation of smooth muscle contraction; IEA:InterPro.
DR   InterPro; IPR018070; Neuromedin-U_amidation-site.
DR   InterPro; IPR042384; NMU.
DR   InterPro; IPR008200; NMU_C.
DR   PANTHER; PTHR15390; PTHR15390; 1.
DR   Pfam; PF02070; NMU; 1.
DR   SMART; SM00084; NMU; 1.
DR   PROSITE; PS00967; NMU; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Amidation; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Hormone; Secreted; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..104
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000262502"
FT   PROPEP          105..139
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000262503"
FT   PROPEP          140..142
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_0000262504"
FT   PROPEP          143..144
FT                   /evidence="ECO:0000269|PubMed:10671478"
FT                   /id="PRO_0000262505"
FT   PEPTIDE         145..167
FT                   /note="Neuromedin-U-23"
FT                   /id="PRO_0000044074"
FT   PROPEP          171..175
FT                   /id="PRO_0000262506"
FT   MOD_RES         167
FT                   /note="Asparagine amide"
FT                   /evidence="ECO:0000269|PubMed:10671478"
FT   VAR_SEQ         105..146
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:12413397, ECO:0000303|Ref.2"
FT                   /id="VSP_021779"
FT   VAR_SEQ         122..146
FT                   /note="Missing (in isoform 3)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_021780"
SQ   SEQUENCE   175 AA;  19558 MW;  D9D90C193A8E5C1C CRC64;
     MQKGSEDTTQ NRCHQHSIGG HSTCGLLLLI ILVSWTSICE GAPFSSPVLG AEDELPLWNG
     IDDACSAVLP DPQLAVSSTL RELCFMVMRM QQKSQGEEKD DFKRFLFHYS KSHDSGNSDI
     TSSVLHPLLQ LLPQLHDRRM KRLTSDEEVQ VPGGVISNGY FLFRPRNGRR SAGFR